Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65154210.31857/S0869813923070038XHKZHOREVIEW AND PROBLEM ARTICLESОБЗОРНЫЕ И ПРОБЛЕМНЫЕ СТАТЬИGCaMP – a Family of Single-Fluorophore Genetically Encoded Calcium IndicatorsGCaMP – семейство однофлуорофорных генетически-кодируемых кальциевых индикаторовErofeevA. I.ЕрофеевА. И.alexandr.erofeew@gmail.comVinokurovE. K.ВинокуровЕ. К.alexandr.erofeew@gmail.comVlasovaO. L.ВласоваО. Л.alexandr.erofeew@gmail.comBezprozvannyI. B.БезпрозванныйИ. Б.alexandr.erofeew@gmail.comLaboratory of molecular neurodegeneration, Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnic UniversityЛаборатория молекулярной нейродегенерации, Институт биомедицинских систем и биотехнологий, Санкт-Петербургский Политехнический университет Петра ВеликогоDepartment of Physiology, University of Texas Southwestern Medical Center at DallasОтделение физиологии, Юго-Западный медицинский центр Техасского университета, Даллас01072023109781984301022025Copyright ©; 2023, А.И. Ерофеев, Е.К. Винокуров, О.Л. Власова, И.Б. БезпрозванныйCopyright ©; 2023, А.И. Ерофеев, Е.К. Винокуров, О.Л. Власова, И.Б. Безпрозванный2023А.И. Ерофеев, Е.К. Винокуров, О.Л. Власова, И.Б. БезпрозванныйА.И. Ерофеев, Е.К. Винокуров, О.Л. Власова, И.Б. Безпрозванныйhttps://journals.eco-vector.com/0869-8139/article/view/651542

Single fluorophore genetically encoded calcium indicators (GECIs) such as GCaMP are widely utilized tools for investigating neuronal activity. Their primary advantage lies in their capacity to provide real-time and highly sensitive responses to fluctuations in intracellular calcium ion concentrations. This characteristic is of significant importance when studying neuronal processes and ensembles, wherein calcium signals play a crucial role in information transmission. This comprehensive review focuses on the GCaMP family, encompassing an analysis of their various types, distinctive features, and potential applications for visualizing neuronal activity. Special attention is dedicated to the ongoing advancements in GCaMP technology, particularly the endeavors to expand their spectral properties and enhance their capability to detect high-frequency spike activity.

Однофлуорофорные генетически кодируемые кальциевые индикаторы, такие как GCaMP, являются широко используемыми инструментами для изучения нейрональной активности. Их главным преимуществом является способность в режиме реального времени и с высокой чувствительностью реагировать на изменения концентрации ионов кальция в клетке. Это особенно важно для исследования нейрональных процессов и нейронных ансамблей, где кальциевые сигналы играют ключевую роль в передаче информации. Данный обзор рассматривает семейство кальциевых индикаторов GCaMP, описывает их разновидности, особенности и перспективы использования для визуализации нейронной активности. Особое внимание уделяется тенденции совершенствования GCaMP, в частности направленной на расширение их спектральных свойств и способности регистрировать высокочастотную спайковую активность.

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