Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65154310.31857/S0869813923070129XIJRPZEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИGenome Instability of Hippocampal and Bone Marrow Cells in Male Mice after the Action of Immobilization and Pheromonal StressorДестабилизация генома клеток гиппокампа и костного мозга самцов мышей после действия иммобилизации и феромонального стрессораShcherbininaV. D.ЩербининаВ. Д.mouse_gene@mail.ruBakulevskiyB. V.БакулевскийБ. В.mouse_gene@mail.ruGlininT. S.ГлининТ. С.mouse_gene@mail.ruDaevE. V.ДаевЕ. В.mouse_gene@mail.ruSaint Petersburg State UniversityСанкт-Петербургский государственный университетPavlov Institute of Physiology Russian Academy of SciencesИнститут физиологии им. И.П. Павлова РАН01072023109784486101022025Copyright ©; 2023, В.Д. Щербинина, Б.В. Бакулевский, Т.С. Глинин, Е.В. ДаевCopyright ©; 2023, В.Д. Щербинина, Б.В. Бакулевский, Т.С. Глинин, Е.В. Даев2023В.Д. Щербинина, Б.В. Бакулевский, Т.С. Глинин, Е.В. ДаевВ.Д. Щербинина, Б.В. Бакулевский, Т.С. Глинин, Е.В. Даевhttps://journals.eco-vector.com/0869-8139/article/view/651543

Different stressors, affecting the cells of target organs, can lead to genomic instability and even disintegration, which can play a role in the formation of post-stress pathologies. We studied the effect of psycho-emotional stressors (immobilization and mouse stress pheromone – 2,5-dimethylpyrazine) on the DNA integrity of hippocampal and bone marrow cell in male mice of CD1, CBA and C3H strains. Cytogenetic and immunocytochemical methods (alkaline comet assay, ana-telophase analysis of mitotic disturbances and analysis of γH2AX foci) were used. It is shown that the classic mouse stressor (immobilization or restraint), similar as 2,5-dimethylpyrazine, damages the genome of the cells of both organs studied. The destabilization of the cell genome of various organs is considered as an essential stage in the development of a stress response, which is an attempt of the organism to adapt to extreme environmental influences.

Стрессоры различной природы, воздействуя на клетки органов-мишеней, способны приводить к геномной нестабильности и даже дезинтеграции, что может играть роль в формировании постстрессорных патологий. На самцах лабораторных мышей различных линий (CD-1, CBA, C3H) цитогенетическими методами (щелочным кометным гель-электрофорезом, ана-телофазным анализом нарушений митоза и иммуноцитохимическим анализом фокусов γH2AX) изучали влияние психоэмоциональных стрессоров (иммобилизации и феромона стресса – 2,5-диметилпиразина) на целостность ДНК клеток гиппокампа и костного мозга. Показано, что как классический стрессор мышей – иммобилизация, так и 2,5-диметилпиразин повреждают геном клеток обоих исследованных органов. Дестабилизация генома клеток различных органов рассматривается как необходимый этап развития стресс-реакции в попытке организма приспособиться к экстремальным воздействиям среды.

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