Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65155010.31857/S0869813923070051XMAGUMEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИReaction of Kidney Medullary Laminin to Prolonged Dehydration of RatsРеакция ламинина мозгового вещества почки на продолжительную дегидратацию крысKhegayI. I.ХегайИ. И.khegay@bionet.nsc.ruFederal Research Center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of SciencesФедеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук01072023109794695301022025Copyright ©; 2023, И.И. ХегайCopyright ©; 2023, И.И. Хегай2023И.И. ХегайИ.И. Хегайhttps://journals.eco-vector.com/0869-8139/article/view/651550

Laminin is the main water-soluble protein in the basement membrane of epithelial tissue. The content of laminin in the renal parenchyma was studied in rats that were on an alternative water diet for 60 h with excessive water intake or a complete lack of drinking water in the food. It has been shown that the osmotic concentration of urine, which develops as a result of water deprivation, is accompanied by a quantitative change in the composition of laminin. It has been established that the amount of laminin beta chain increases in the kidney medulla of dehydrated rats. This effect is observed only in WAG rats with normally expressed vasopressin gene and is absent in mutant Brattleboro rats unable to synthesize the hormone. The increase in the level of beta-laminin also does not extend to the cortical substance. Based on the original and new data, it is assumed that laminin, as a key regulatory element in the basement membrane of the tubular epithelium, participates in the adaptive reaction of the concentrating system of the kidney to conditions of prolonged dehydration. Hyperhydration does not affect the level of laminin in the renal parenchyma.

Ламинин является основным водорастворимым белком базальной мембраны эпителиальной ткани. Исследовано содержание ламинина в почечной паренхиме у крыс, находившихся в течение 60 ч на альтернативной водной диете с избыточным потреблением воды либо полным отсутствием питьевой воды в пище. Показано, что осмотическое концентрирование мочи, развивающееся вследствие водной депривации, сопровождается количественным изменением состава ламинина. Установлено, что в мозговом веществе почки дегидратированных крыс возрастает количество бета-цепи ламинина. Данный эффект наблюдается только у крыс линии WAG с нормально экспрессирующимся геном вазопрессина и отсутствует у мутантных крыс линии Brattleboro, неспособных синтезировать гормон. Повышение уровня бета-ламинина также не распространяется на корковое вещество. На основании исходных и новых данных предполагается, что ламинин как ключевой регуляторный элемент в составе базальной мембраны канальцевого эпителия участвует в адаптивной реакции концентрирующей системы почки к условиям продолжительной дегидратации. Гипергидратация не влияет на уровень ламинина в почечной паренхиме.

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