Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65155910.31857/S0869813923060080WHITZFEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИImpakt of Ozone on the Oxyge Affinity Blood Properties and Prooxidant–Antioxidant Balance under Effect of H2S-Generating SystemВлияние озона на кислородсвязывающие свойства крови и ее прооксидантно-антиоксидантный баланс в условиях воздействия на H2S-генерирующую системуZinchukV. V.ЗинчукВ. В.zinchuk@grsmu.byBiletskayaE. S.БилецкаяЕ. С.zinchuk@grsmu.byGrodna State Medical UniversityГродненский государственный медицинский университет01062023109676077001022025Copyright ©; 2023, В.В. Зинчук, Е.С. БилецкаяCopyright ©; 2023, В.В. Зинчук, Е.С. Билецкая2023В.В. Зинчук, Е.С. БилецкаяВ.В. Зинчук, Е.С. Билецкаяhttps://journals.eco-vector.com/0869-8139/article/view/651559

Hydrogen sulfide belongs to the group of signaling agents called gaseous transmitters and plays an important role in many physiological processes, in particular, in the realization of oxygen-dependent mechanisms. The aim of this study was to evaluate the significance of hydrogen sulfide in the effect of ozone on the oxygen affinity blood properties and the prooxidant-antioxidant balance in vitro experiment. Ozone (6 mg/L concentration) and drugs that affect the synthesis of hydrogen sulfide (propargylglycine, sodium hydrosulfide and its combination with nitroglycerin) were used. The use of propargylglycine, an inhibitor of the synthesis of hydrogen sulfide, leads to a decrease in the effect of ozone on the blood oxygen transport function (decrease in PO2, SO2, P50real). When sodium hydrosulfide is added, the effect of this gas on these parameters does not increase, but in its combination with nitroglycerin, the effect of ozone on the blood oxygen transport function increases. Propargylglycine does not affect the prooxidant-antioxidant balance under the conditions of the experiment, and donors of hydrogen sulfide and nitrogen monoxide increase the activity of catalase. Propargylglycine under the action of ozone leads to a decrease in the level of nitrate/nitrite, and sodium hydrosulfide increases their concentration. The combination of sodium hydrosulfide and nitroglycerin leads to the accumulation of hydrogen sulfide in the blood plasma.

Сероводород относится к группе сигнальных агентов, называемых газотрансмиттерами, и играет важную роль во многих физиологических процессах, в частности в реализации кислородзависимых механизмов. Цель данного исследования – изучить значение сероводорода в эффекте озона на кислородсвязывающие свойства крови и прооксидантно-антиоксидантный баланс в опытах in vitro. Использовались озон (концентрация 6 мг/л) и препараты, влияющие на синтез сероводорода (пропаргилглицин, гидросульфид натрия и его комбинация с нитроглицерином). Применение ингибитора синтеза сероводорода пропаргилглицина приводило к уменьшению эффекта озона на кислородтранспортную функцию крови (снижение РО2, SO2, Р50реал). При добавлении гидросульфида натрия не усиливалось воздействие этого газа на данные параметры, но в его комбинации с нитроглицерином увеличивалось влияние озона на кислородтранспортную функцию крови. Пропаргилглицин не влиял на прооксидантно-антиоксидантный баланс в условиях проведения опытов, а доноры сероводорода и монооксида азота увеличивали активность каталазы. Пропаргилглицин в условиях действия озона приводил к уменьшению уровня нитрат/нитритов, а гидросульфид натрия увеличивал их концентрацию. Комбинация гидросульфида натрия и нитроглицерина способствовала росту сероводорода в плазме крови.

ozonebloodoxygengaseous transmitternitrogen monoxidenitroglycerinhydrogen sulfidepropargylglycineозонкровькислородгазотрансмиттермонооксид азотанитроглицеринсероводородпропаргилглицинTirelli U, Franzini M, Valdenassi L, Pisconti S, Taibi R, Torrisi C, Pandolfi S, Chirumbolo S (2021) Fatigue in post-acute sequelae of SARS-CoV2 (PASC) treated with oxygen-ozone autohemotherapy – preliminary results on 100 patients. Eur Rev Med Pharmacol Sci 25(18): 5871–5875. https://doi.org/10.26355/eurrev_202109_26809Pchepiorka R, Moreira MS, Lascane NADS, Catalani LH, Allegrini S Jr, de Lima NB, Gonçalves EF (2020) Effect of ozone therapy on wound healing in the buccal mucosa of rats. Arch Oral Biol (119): 104889. https://doi.org/:10.1016/j.archoralbio.2020.104889Smith NL, Wilson AL, Gandhi J, Vatsia S, Khan SA (2017) Ozone therapy: an overview of pharmacodynamics, current research, and clinical utility. Med Gas Res 7(3): 212–219. https://doi.org/10.4103/2045-9912.215752Robert B, Subramaniam S (2022) Gasotransmitter-Induced Therapeutic Angiogenesis: A Biomaterial Prospective. ACS Omega 7(50): 45849–45866. https://doi.org/10.1021/acsomega.2c05599Głowacka U, Brzozowski T, Magierowski M (2020) Synergisms, discrepancies and Interactions between Hydrogen Sulfide and Carbon Monoxide in the Gastrointestinal and Digestive System Physiology, Pathophysiology and Pharmacology. Biomolecules 10(3): 445–460. https://doi.org/10.3390/biom10030445Yang YW, Deng NH, Tian KJ, Liu LSh, Wang Z, Wei DH, Liu HT, Jiang ZhS (2022) Development of hydrogen sulfide donors for anti-atherosclerosis therapeutics research: Challenges and future priorities. Front Cardiovasc Med (9): 909178. https://doi.org/10.3389/fcvm.2022.909178Зинчук ВВ, Билецкая ЕС, Гуляй ИЕ (2021) Влияние озона на кислородтранспортную функцию крови и прооксидантно-антиоксидантный баланс в условиях изменения образования монооксида азота в экспериментах in vitro. Рос физиол журн им ИМ Сеченова 107(1): 16–27. [Zinchuk VV, Biletskaya ES, Gulyai IE (2021) Effect of ozone on blood oxygen transport function and pro-oxidant–antioxidant balance in under conditions of changing nitrogen monoxide formation in vitro experiments. Russ J Physiol 107(1): 16–27. (In Russ)]. https://doi.org/10.31857/S0869813921010106Mys LA, Strutynska NA, Goshovska YV, Sagach VF (2020) Stimulation of the endogenous hydrogen sulfide synthesis suppresses oxidative-nitrosative stress and restores endothelial-dependent vasorelaxation in old rats. Can J Physiol Pharmacol 98(5): 275–281. https://doi.org/10.1139/cjpp-2019-0411Saveringhaus JW (1966) Blood gas calculator. J Appl Physiol 21(5): 1108–1116. https://doi.org/10.1152/jappl.1966.21.3.1108Mendes R, Cardoso C, Pestana C (2009) Measurement of malondialdehyde in fish: A comparison study between HPLC methods and the traditional spectrophotometric test. Food Chem (112): 1038–1045. https://doi.org/10.1016/j.foodchem.2008.06.052Diplock AT, Symons MCR, Rice-Evans CA (1991) Techniques in free radical research. In: Lab Techn in Biochem and Mol Biol (22): 290.Королюк МА, Иванова ЛИ, Майорова ИГ, Токарева ВЕ (1988) Метод определения активности каталазы. Лаб дело (1): 16–19. [Korolyuk MA, Ivanova LI, Mayorova IG, Tokareva VE (1988) Method for determination of catalase activity. Lab Delo (1): 16–19. (In Russ)]. https://pubmed.ncbi.nlm.nih.gov/2451064/Taylor SL, Lamden MP, Tappel AL (1976) Sensitive fluorometric method for tissue tocopherol analysis. Lipids 11(7): 530–538. https://doi.org/10.1007/BF02532898Bryan NS, Grisham MB (2007) Methods to detect nitric oxide and its metabolites in biological samples. Free Radic Biol Med 43(5): 645–657. https://doi.org/10.1016/j.freeradbiomed.2007.04.026Norris EJ, Culberson CR, Narasimhan S, Clemens MG (2011) The liver as central regulator of hydrogen sulfide. Shock 36(3): 242– 250. https://doi.org/10.1097/SHK.0b013e3182252ee7Ross BK, Hlastala MP, Frank R. (1979) Lack of ozone effects on oxygen hemoglobin affinity. Arch Environ Health 34(3): 161–163.https://doi.org/10.1080/00039896.1979.10667389Giunta R, Coppola A, Luongo C, Sammartino A, Guastafierro S, Grassia A, Giunta L, Mascolo L, Tirelli A, Coppola L (2001) Ozonized autohemotransfusion improves hemorheological parameters and oxygen delivery to tissues in patients with peripheral occlusive arterial disease. Ann Hematol 80(12): 745–748.https://doi.org/10.1007/s002770100377Munteanu C, Rotariu M, Turnea M, Dogaru G, Popescu C, Spînu A, Andone I, Postoiu R, Ionescu EV, Oprea C, Albadi I, Onose G (2022) Recent Advances in Molecular Research on Hydrogen Sulfide (H2S) Role in Diabetes Mellitus (DM)-A Systematic Review. Int J Mol Sci 23(12): 6720. https://doi.org/10.3390/ijms23126720Fadyukova OE, Koshelev VB Effect of Hydrogen (2020) Sulfide on Deformability of Rat Erythrocytes. Bull Exp Biol Med 169(6): 664–667.https://doi.org/10.1007/s10517-020-04965-9Cortese-Krott MM (2020) Red Blood Cells as a “Central Hub” for Sulfide Bioactivity: Scavenging, Metabolism, Transport, and Cross-Talk with Nitric Oxide. Antioxid Redox Signal 33(18): 1332–1349.https://doi.org/10.1089/ars.2020.8171Kolupaev YE, Yemets AI, Yastreb TO, Blume YB (2023) The role of nitric oxide and hydrogen sulfide in regulation of redox homeostasis at extreme temperatures in plants. Front Plant Sci (14): 1128439.https://doi.org/10.3389/fpls.2023.1128439Bieza S, Mazzeo A, Pellegrino J, Doctorovich F (2022) H2S/Thiols, NO•, and NO–/HNO: Interactions with Iron Porphyrins. ACS Omega 7(2): 1602–1611.https://doi.org/10.1021/acsomega.1c06427Wang G, Huang Y, Zhang N, Liu W, Wang C, Zhu X, Ni X (2021) Hydrogen Sulfide Is a Regulator of Hemoglobin Oxygen-Carrying Capacity via Controlling 2,3-BPG Production in Erythrocytes. Oxid Med Cell Longev 2021: 8877691.https://doi.org/10.1155/2021/8877691Kolluru GK, Prasai PK, Kaskas AM, Letchuman V, Pattillo B (2016) Oxygen tension, H2S, and NO bioavailability: is there an interaction? J Appl Physiol 120(2): 263–270.https://doi.org/10.1152/japplphysiol.00365.2015Hancock SE, Maccarone AT, Poad BLJ, Trevitt AJ, Mitchell TW, Blanksby SJ (2019) Reaction of ionised steryl esters with ozone in the gas phase. Chem Phys Lipids (221): 198–206.https://doi.org/10.1016/j.chemphyslip.2018.12.013Bocci V, Borrelli E, Travagli V, Zanardi I (2009) The ozone paradox: ozone is a strong oxidant as well as a medical drug. Med Res Rev 29(4): 646–682.https://doi.org/10.1002/med.20150Wen Q, Chen Q (2020) An Overview of Ozone Therapy for Treating Foot Ulcers in Patients With Diabetes. Am J Med Sci 360(2): 112–119.https://doi.org/10.1016/j.amjms.2020.05.012Christie AE, Fontanilla TM, Roncalli V, Cieslak MC, Lenz PH (2014) Diffusible gas transmitter signaling in the copepod crustacean Calanus finmarchicus: identification of the biosynthetic enzymes of nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S) using a de novo assembled transcriptome. Gen Comp Endocrinol (202): 76–86.https://doi.org/10.1016/j.ygcen.2014.04.003Feng J, Lu X, Li H, Wang S (2022) The roles of hydrogen sulfide in renal physiology and disease states. Renal Fail 44(1): 1289–1308.https://doi.org/10.1080/0886022X.2022.2107936Rajendran S, Shen X, Glawe J, Kolluru GK, Kevil CG (2019) Nitric Oxide and Hydrogen Sulfide Regulation of Ischemic Vascular Growth and Remodeling. Compr Physiol 9(3): 1213–1247.https://doi.org/10.1002/cphy.c180026Colakerol A, Temiz MZ, Tavukcu HH, Aykan S, Ozsoy S, Sahan A, Kandirali E, Semercioz A (2021) Effects of ozone treatment on penile erection capacity and nitric oxide synthase levels in diabetic rats. Int J Impot Res 33(5): 1–8.https://doi.org/10.1038/s41443-020-0301-1Orlandin JR, Pinto Santos SI, Machado LC, Neto PF, Bressan FF, Godoy Pieri NC, Recchia K, de Paula Coutinho M, Ferreira Pinto PA, Santucci A, Travagli V, Ambrosio CE (2022) Evaluation of targeted oxidative stress induced by oxygen-ozone in vitro after ischemic induction. Redox Rep 27(1): 259–269. https://doi.org/10.1080/13510002.2022.2143104Sojitra B, Bulani Y, Putcha UK, Kanwal A, Gupta P, Kuncha M, Banerjee SK (2012) Nitric oxide synthase inhibition abrogates hydrogen sulfide-induced cardioprotection in mice. Mol Cell Biochem 360(1–2): 61–69. https://doi.org/10.1007/s11010-011-1044-6