Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65158710.31857/S0869813923030044FSRFDJEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИEffects of Assisted Reproductive Technologies on Social Behavior of BTBR Mice – A Model of Autism Spectrum DisorderЭффекты вспомогательных репродуктивных технологий на социальное поведение мышей линии BTBR – модели расстройств аутистического спектраRozhkovaI. N.РожковаИ. Н.amstis@yandex.ruNaprimerovV. A.НапримеровВ. А.amstis@yandex.ruKozenevaV. S.КозеневаВ. С.amstis@yandex.ruYatsukT. A.ЯцукТ. А.amstis@yandex.ruLebedevaD. A.ЛебедеваД. А.amstis@yandex.ruRakhmanovaT. A.РахмановаТ. А.amstis@yandex.ruIgoninaT. N.ИгонинаТ. Н.amstis@yandex.ruBrusentsevE. Yu.БрусенцевЕ. Ю.amstis@yandex.ruOkotrubS. V.ОкотрубС. В.amstis@yandex.ruAmstislavskyS. Ya.АмстиславскийС. Я.amstis@yandex.ruInstitute of Cytology and Genetics Siberian Branch of the Russian Academy of SciencesИнститут цитологии и генетики Сибирского отделения Российской академии наукNovosibirsk State UniversityНовосибирский национальный исследовательский государственный университет01032023109331533301022025Copyright ©; 2023, И.Н. Рожкова, С.В. Окотруб, Е.Ю. Брусенцев, Т.Н. Игонина, Т.А. Рахманова, Д.А. Лебедева, Т.А. Яцук, В.С. Козенева, В.А. Напримеров, С.Я. АмстиславскийCopyright ©; 2023, И.Н. Рожкова, С.В. Окотруб, Е.Ю. Брусенцев, Т.Н. Игонина, Т.А. Рахманова, Д.А. Лебедева, Т.А. Яцук, В.С. Козенева, В.А. Напримеров, С.Я. Амстиславский2023И.Н. Рожкова, С.В. Окотруб, Е.Ю. Брусенцев, Т.Н. Игонина, Т.А. Рахманова, Д.А. Лебедева, Т.А. Яцук, В.С. Козенева, В.А. Напримеров, С.Я. АмстиславскийИ.Н. Рожкова, С.В. Окотруб, Е.Ю. Брусенцев, Т.Н. Игонина, Т.А. Рахманова, Д.А. Лебедева, Т.А. Яцук, В.С. Козенева, В.А. Напримеров, С.Я. Амстиславскийhttps://journals.eco-vector.com/0869-8139/article/view/651587

The present work is the first attempt to study the effect of such assisted reproductive technologies (ARTs), as in vitro culture of preimplantation embryos on the social behavior of offspring, using BTBR mice (BTBR T+Itpr3tf/J) as an idiopathic model of a-utism. The C57BL/6J mice were used as controls. Social behavior was studied in adult offspring mice obtained after in vitro culture and embryo transfer (ET) (groups ET-C57BL/6J and ET-BTBR). The BTBR mice demonstrated the reduced levels of social recognition and affiliation compared to C57BL/6J mice. The social affiliation and recognition test revealed that ET-C57BL/6J males had a high level of social interaction, which was of similar magnitude as in naturally conceived C57BL/6J males. However, ET-C57BL/6J and ET-BTBR females demonstrated a decrease in interest to a new stranger compared to naturally conceived controls of the same sex, which was revealed in a social recognition session of this test. Thus, the in vitro culture affected the social recognition in the offspring of C57BL/6J and BTBR mice, but only in females.

Работа является первым исследованием влияния такой вспомогательной репродуктивной технологии (ВРТ), как культивирование in vitro преимплантационных эмбрионов на социальное поведение потомков мышей линии BTBR (BTBR T + Itpr3tf/J), которая представляет собой идиопатическую модель аутизма. В качестве контроля были использованы мыши C57BL/6J. У взрослых потомков мышей, полученных в результате естественного спаривания (группы C57BL/6J и BTBR), либо после культивирования in vitro и переноса эмбрионов соответствующих линий мышей псевдобеременным самкам C57BL/6J (группы ВРТ-C57BL/6J и ВРТ-BTBR), было изучено социальное поведение. При межлинейном сравнении было показано, что мыши BTBR имеют сниженные уровни социального распознавания и мотивации по сравнению с мышами C57BL/6J. У самок ВРТ-C57BL/6J и ВРТ-BTBR было установлено снижение интереса к новому партнеру в сессии на социальное распознавание по сравнению с естественно зачатыми животными того же пола. Результаты поведенческого теста свидетельствуют о влиянии культивирования in vitro на социальное распознавание у потомков мышей линий C57BL/6J и BTBR, но только у самок.

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