Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65173610.31857/S0869813924100116VQSNGWEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleBehavioural and electrophysiological features of WAG/Rij rats with different forms of genetic epilepsyПоведенческие и электрофизиологические особенности крыс линии WAG/RIJ с разными формами генетической эпилепсииAbbasovaК. R.АббасоваК. Р.
Russian Federation
abbasovakr@my.msu.ruKuzhugetS. М.КужугетC. М.
Russian Federation
abbasovakr@my.msu.ruTsybaЕ. Т.ЦыбаЕ. Т.
Russian Federation
abbasovakr@my.msu.ruLomonosov Moscow State UniversityМосковский государственный университет имени М. В. Ломоносова15102024110101733175102022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/0869-8139/article/view/651736

WAG/Rij rats are widely used as a genetic model of absence epilepsy. Approximately 15–50% rats of the strain are susceptible to audiogenic seizures. WAG/Rij rats demonstrate depressive-like behavior. After preliminary sound provocation an increased level of anxiety was found in audiogenic susceptible WAG/Rij subgroup. Electrophysiological and behavioral studies suggest the involvement of the dopaminergic system in both absence and audiogenic epilepsy. An increased binding density to dopamine receptors was found in the dorsal striatum subregions in audiogenic prone rats compared to non-audiogenic. The study aims were (1) to determine whether behavioral changes in WAG/Rij rats were genetically determined or induced by prior sound stimulation; (2) how regions of the dorsal striatum with different density of dopamine receptors in subpopulations of WAG/Rij rats are involved in the absence epilepsy control. The study was conducted using two rat groups: WAG/Rij-nonAGS (absence epilepsy) and WAG/Rij-AGS (mixed epilepsy). The study was performed using tests: “Elevated plus maze”, “Forced swimming” and “Three chamber sociability test”. High-frequency deep brain stimulation was performed for evaluation of dorsal striatum involvement in the absence seizure control. After experiments animals were tested for the susceptibility to audiogenic seizures. It demonstrated that the increased level of anxiety in WAG/Rij-AGS rats is genetically determined, while depressive-like behavior in WAG/Rij rats is not dependent on a predisposition to audiogenic seizures. Deviations in social behavior were observed in WAG/Rij-AGS rats. Stimulation of the dorsal striatum indicates differences in the control of absence and mixed forms of epilepsy in the

Крысы линии WAG/Rij являются генетической моделью абсансной эпилепсии. Около 15–50% крыс данной линии предрасположены к аудиогенным судорогам. Крысы WAG/Rij демонстрируют депрессивноподобное поведение, а в субпопуляции крыс WAG/Rij с предрасположенностью к аудиогенным судорогам отмечается повышенный уровень тревожности после предварительной звуковой стимуляции. Электрофизиологические и поведенческие исследования указывают на вовлеченность дофаминергической системы как в абсансную, так и в аудиогенную эпилепсию. В дорсальном стриатуме обнаружена область, где плотность дофаминовых рецепторов у предрасположенных к аудиогенным судорогам крыс WAG/Rij (WAG/Rij-AGS) была выше, чем у неаудиогенных крыс WAG/Rij (WAG/Rij-nonAGS). Целью исследования было выяснить: (1) являются ли поведенческие изменения у крыс WAG/Rij генетически обусловленными или вызваны предварительной звуковой стимуляцией; (2) как вовлечены области дорсального стриатума с разной плотностью дофаминовых рецепторов в контроль абсансной эпилепсии у разных субпопуляций крыс WAG/Rij. Исследование проводили на самцах крыс WAG/Rij, разделенных на группы: WAG/Rij-nonAGS (с абсансной эпилепсией) и WAG/Rij-AGS (со смешанной формой эпилепсии – абсансной и аудиогенной). Использовали тесты “Приподнятый крестообразный лабиринт”, “Вынужденное плавание” и “Трехкамерный социальный лабиринт”. Для оценки вовлеченности дорсального стриатума в контроль абсансных судорог применяли метод прямой высокочастотной стимуляции мозга. Тестирование животных на аудиогенную эпилепсию проводили после окончания экспериментов. Показали, что повышенный уровень тревожности у крыс WAG/Rij-AGS генетически обусловлен, а депрессивноподобное поведение у крыс WAG/Rij не зависит от предрасположенности к аудиогенным судорогам. У крыс WAG/Rij-AGS зарегистрировали отклонения в социальном поведении. Высокочастотная стимуляция области дорсального стриатума, в которой отличается плотность дофаминовых рецепторов у аудиогенных и неаудиогенных групп крыс, указала на различия вовлеченности дорсального стриатума в контроль абсансной и смешанной форм эпилепсии у WAG/Rij-AGS и WAG/Rij-nonAGS субпопуляций.

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