Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65175010.31857/S0869813924090116AJHJYKEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleParticipation of Ca2+-acceptor proteins in the mechanisms of the exo-endocytic cycle of synaptic vesicles in the motor nerve endings of the somatic musculature of the earthworm Lumbricus terrestrisУчастие Сa2+-акцепторных белков в механизмах экзо-эндоцитозного цикла синаптических везикул в двигательных нервных окончаниях соматической мускулатуры дождевого червя Lumbricus terrestrisNurullinL. F.НуруллинЛ. Ф.
Russian Federation

Kazan Institute of Biochemistry and Biophysics

Казанский институт биохимии и биофизики

lenizn@yandex.ruAlmazovN. D.АлмазовН. Д.
Russian Federation
lenizn@yandex.ruVolkovE. M.ВолковЕ. М.
Russian Federation
euroworm@mail.ruFederal Research Center “Kazan Scientific Center of Russian Academy of Sciences”Федеральный исследовательский центр “Казанский научный центр Российской академии наук”Kazan State Medical UniversityКазанский государственный медицинский университет1509202411091430143902022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/0869-8139/article/view/651750

Using fluorescence microscopy, we studied the participation of Ca2+-acceptor proteins in the processes of the exo-endocytotic cycle of neurotransmitter quantal secretion in the neuromuscular junction of the somatic muscle of the earthworm Lumbricus terrestris. Inhibition of calcineurin, calmodulin and Ca2+/calmodulin dependent protein kinases led to an increase in the process of endocytosis. Blocking the phosphorylation of synaptic proteins enhances the process of endocytosis, causes an increase in the size of the total vesicular pool and accelerates the turnover of synaptic vesicles. It can be concluded that calcium modulation of vesicle exo-endocytosis at the synapses of the evolutionarily primary somatic muscles of annelids occurs with the participation of calcineurin, calmodulin and Ca2+/calmodulin-dependent protein kinases.

Методами флуоресцентной микроскопии изучали участие Са2+-акцепторных белков в процессах экзо-эндоцитозного цикла квантовой секреции медиатора в нервно-мышечных синапсах соматической мышцы дождевого червя Lumbricus terrestris. Ингибирование кальциневрина, кальмодулина и Са2+/кальмодулин-зависимых протеинкиназ приводило к усилению процесса эндоцитоза. Блокирование фосфорилирования синаптических белков усиливало процесс эндоцитоза, вызывало увеличение размеров общего везикулярного пула и ускоряло кругооборот синаптических везикул. Можно сделать вывод, что кальциевая модуляция экзо-эндоцитоза везикул в синапсах эволюционно-первичной соматической мускулатуры аннелид происходит при участии кальциневрина, кальмодулина и Са2+/кальмодулин-зависимых протеинкиназ.

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