Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences68627510.31857/S0869813925050063TNSLRFEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleThermosensitive TRP-Ion Channels Gene Expression in the Spleen in Normo- and Hypertensive Rats. Effect of the Cold and the Peripheral TRPM8 Ion Channel ActivationЭкспрессия генов термочувствительных TRP-ионных каналов в селезенке у нормо- и гипертензивных крыс. Влияние холода и активации периферического ионного канала TRPM8EvtushenkoA. A.ЕвтушенкоА. А.
Russian Federation
evtushenkoaa@neuronm.ruVoronovaI. P.ВороноваИ. П.
Russian Federation
evtushenkoaa@neuronm.ruKozyrevaT. V.КозыреваТ. В.
Russian Federation
evtushenkoaa@neuronm.ruScientific Research Institute of Neurosciences and MedicineНаучно-исследовательский институт нейронаук и медицины1505202511157447602806202528062025Copyright ©; 2025, Russian Academy of SciencesCopyright ©; 2025, Российская академия наук2025Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/0869-8139/article/view/686275

Arterial hypertension leads to changes in the functioning of various organism systems, including the immune system. TRP-ion channels are increasingly attracting attention as targets for the correction of visceral organs, including for therapeutic purposes. The gene expression of the thermosensitive TRP-ion channels (TRPM8, TRPA1, TRPV1, TRPV2, TRPV3 and TRPV4) was studied in the immunocompetent organ, the spleen, using quantitative RT-PCR in normotensive and hypertensive animals under normal conditions, during cooling, and stimulation of the peripheral cold-sensitive ion channel TRPM8. The investigated genes express differently in the spleen of animals of both lines. The expression of cold-sensitive ion channel genes TRPM8 and TRPA1 is reduced in the spleen of hypertensive rats. This is consistent with obtained data on the decrease expression of the TRPM8 ion channel gene in the hypothalamus of hypertensive animals. Deep cooling of the organism, as well as activation of the peripheral (cutaneous) TRPM8-ion channel by menthol, lead to increase expression of the TRPA1 and TRPV1 ion channels genes in the spleen of hypertensive rats, without affecting the expression of genes in normotensive animals. Considering that the ion channels TRPA1 and TRPV1 are involved in inflammation processes, this may indicate a change in the nature of inflammatory reactions in hypertensive animals when organism is exposed to cold. The obtained data expand knowledge about the representation of thermosensitive TRP-ion channels in immunocompetent organs and may indicate a change in the immune status of organism with arterial hypertension.

Артериальная гипертензия приводит к изменениям функционирования разных систем организма, включая иммунную систему. TRP-ионные каналы все больше привлекают внимание в качестве мишеней для коррекции работы висцеральных органов, в том числе с терапевтическими целями. В настоящей работе исследовалось представительство термочувствительных TRP-ионных каналов в иммунокомпетентном органе – селезенке – методом количественного ОТ-ПЦР у нормо- и гипертензивных животных в норме, при охлаждении и стимуляции периферического холодочувствительного ионного канала TRPM8. Показано, что гены исследованных TRP-ионных каналов (TRPM8, TRPA1, TRPV1, TRPV2, TRPV3 и TRPV4) в разной степени экспрессируются в селезенке у животных обеих линий. У крыс с артериальной гипертензией в селезенке снижена экспрессия генов холодочувствительных ионных каналов TRPM8 и TRPA1. Это согласуется с ранее полученными данными о снижении экспрессии гена ионного канала TRPM8 в гипоталамусе у гипертензивных животных. Глубокое охлаждение организма, как и активация периферического (кожного) холодочувствительного ионного канала TRPМ8 ментолом, приводила к повышению экспрессии генов ионных каналов TRPA1 и TRPV1 в селезенке гипертензивных крыс, не оказывая влияния на экспрессию генов у нормотензивных животных. Учитывая вовлеченность ионных каналов TRPA1 и TRPV1 в процессы воспаления, это может указывать на изменение характера воспалительных реакций у гипертензивных животных при действии холода на организм. Полученные данные могут свидетельствовать о вовлеченности TRP-ионных каналов селезенки в изменение иммунного статуса организма при артериальной гипертензии.

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