N.N. Priorov Journal of Traumatology and OrthopedicsN.N. Priorov Journal of Traumatology and Orthopedics0869-86782658-6738Eco-Vector4226810.17816/vto202027352-59Research ArticleCalcium phosphate and composite materials functionalization of bioactive agents for its target delivery to the boneKuvshinovaEkaterina A.<p>junior researcher</p>beliay@mail.ruhttps://orcid.org/0000-0003-4331-239XPetrakovaNatalia V.<p>candidate of sciences, researcher</p>petrakova.nv@mail.ruSergeevaNatalya S.<p>doctor of biological sciences, professor, head of the department</p>prognoz.06@mail.ruSviridovaIrina K.<p>candidate of biological sciences, leading researcher</p>prognoz.06@mail.ruKirsanovaValentina A.<p>candidate of biological sciences, researcher</p>prognoz.06@mail.ruAhmedovaSuraya A.<p>candidate of biological sciences, researcher</p>prognoz.06@mail.ruKaralkinPavel A.<p>candidate of biological sciences, senior researcher</p>pkaralkin@gmail.comTeterinaAnastasia Y.<p>candidate of technical sciences, junior researcher</p>kinskusha@mail.ruKomlevVladimir S.<p>PhD, corresponding member of the Russian Academy of Sciences, director</p>komlev@mail.ruP.A.Herzen Moscow Oncology Research Institute — Branch of the National Medical Research Radiological Centre of the Ministry of Healthcare of the Russian FederationInstitutution of Russian Academy of Science A.A. Baikov Institute of Metallurgy and Material Science2612202027352590508202025082020Copyright © 2020, Eco-Vector2020<p><strong>Aim of the study.</strong> The development of the method of octacalcium phosphate (OCP) and mineral-polymer composite material functionalization with biological agents (human platelet lysate (PL) growth factors and antibiotic vancomycin) by the biomimetic coprecipitation principle technique.</p>
<p><strong>Materials and methods.</strong> The OCP and the mineral-polymer composite matrices (sodium alginate / gelatin / OCP) functionalization was obtained by biomimetic coprecipitation of calcium phosphates and the bioactive molecules on their surface. The materials structure was examined by electron microscopy. The functionalization efficiency was determined by measurement of the incorporated compounds in solution, as well as by analysis of their release over the 8 days. The antimicrobial activity of vancomycin functionalized samples was evaluated by in vitro disk diffusion method against the Staphylococcus aureus strain.</p>
<p><strong>Results.</strong> The evaluation of incorporated molecules release showed that the OCP functionalization with vancomycin is more effective than PL. The antibiotic release had continued for three days, while PL growth factors only for 30 minutes. The incorporated into a composite matrix vancomycin was completely released within 24 h. In vitro study of the functionalized composite samples showed growth delay of the Staphylococcus aureus strain in dependence on antibiotic content.</p>
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