Current Pharmaceutical DesignCurrent Pharmaceutical DesignCurrent Pharmaceutical Design1381-61281873-4286Bentham Science64579910.2174/0113816128296935240502070245Immunology, Inflammation & AllergyResearch ArticleProtective Effects of the Aqueous Extract of Malva sylvestris Plant against the Lethality and Histopathological Damage in Experimental Envenoming of Hemiscorpius lepturus Venom in Balb/c MiceAhmadiSayyedinfo@benthamscience.netYousofpourMohammadinfo@benthamscience.netKamalinejadMohammadinfo@benthamscience.netOtroshiArmaninfo@benthamscience.netAmiriFereshtehinfo@benthamscience.netAlipourAbbasinfo@benthamscience.netZareinejadMohammadinfo@benthamscience.netShahbazzadehDelavarinfo@benthamscience.netStudent Research Committee, Mazandaran University of Medical SciencesDepartment of Persian Medicine, Faculty of Medicine, Mazandaran University of Medical SciencesDepartment of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical SciencesIsfahan Clinical Toxicology Research Center,, Isfahan University of Medical SciencesDepartment of Anatomy, Molecular and Cell Biology Research Center, Mazandaran University of Medical SciencesCommunity Medicine Department, Medical Faculty, Mazandaran University of Medical SciencesVenom and Biotherapeutics Molecules Lab., Medical Biotechnology Department,, Biotechnology Research Center, Pasteur Institute of Iran0106202430211699170411012025Copyright ©; 2024, Bentham Science Publishers2024Bentham Science Publishershttps://journals.eco-vector.com/1381-6128/article/view/645799

Introduction:Hemiscorpius lepturus envenomation is a serious health problem in the southern provinces of Iran. The antiserum produced in Iran to counteract this scorpion venom is not entirely effective due to the risk of anaphylactic shock and other adverse effects.

Methods:Therefore, more efficient alternatives to treat patients deserve attention, and plants are extensively good candidates to be studied. This study aimed to assess the potential of the aqueous fraction of Malva sylvestris in inhibiting the toxic effects of H. lepturus venom. Injection of sub-lethal dose of H. lepturus venom leads to severe tissue damage in vital organs including the kidney, liver, heart and intestine, after 24 hours.

Results:By injecting 80 mg of the aqueous extract of M. sylvestris into the peritoneum helped treat the damaged tissues caused by H. lepturus venom in mice.

Conclusion:Thus, Malva sylvestris could serve as an alternative treatment for scorpion sting envenomation and may be used as a drug to neutralize relevant toxic effects in patients stung by H. lepturus.

Malva sylvestris (PANIRAK)Hemiscorpius lepturusPersian medicinescorpion envenomationdrug leadacute kidney injury.Dehghani R, Kamiabi F, Mohammadi M. Scorpionism by Hemiscorpius spp. in Iran: A review. J Venom Anim Toxins Incl Trop Dis 2018; 24(1): 8. doi: 10.1186/s40409-018-0145-z PMID: 29507581Dizaji R, Sharafi A, Pourahmad J, Vatanpour S, Hosseini MJ, Vatanpour H. The effects of Hemiscorpius lepturus induced-acute kidney injury on PGC-1α gene expression: From induction to suppression in mice. Toxicon 2020; 174: 57-63. doi: 10.1016/j.toxicon.2019.12.154 PMID: 31887316Pipelzadeh MH, Jalali A, Taraz M, Pourabbas R, Zaremirakabadi A. An epidemiological and a clinical study on scorpionism by the Iranian scorpion Hemiscorpius lepturus. Toxicon 2007; 50(7): 984-92. doi: 10.1016/j.toxicon.2007.07.018 PMID: 17854855Dehghani R, Khamechian T, Vazirianzadeh B, Vatandoost H, Moravvej S. Toxic effects of scorpion, Hemiscorpius lepturus (Hemiscorpiidae) venom on mice. J Anim Plant Sci 2012; 22(3): 593-6.Valavi E, Ansari AMJ. Hemolytic uremic syndrome following Hemiscorpius lepturus (scorpion) sting. Indian J Nephrol 2008; 18(4): 166-8. doi: 10.4103/0971-4065.45293 PMID: 20142930Jalali A, Pipelzadeh MH, Taraz M, Khodadadi A, Makvandi M, Rowan EG. Serum TNF-α levels reflect the clinical severity of envenomation following a Hemiscorpius lepturus sting. Eur Cytokine Netw 2011; 22(1): 5-10. doi: 10.1684/ecn.2011.0278 PMID: 21411417Yardehnavi N, Behdani M, Bagheri KP, et al. A camelid antibody candidate for development of a therapeutic agent against Hemiscorpius lepturus envenomation. FASEB J 2014; 28(9): 4004-14. doi: 10.1096/fj.13-247478 PMID: 24891523Zargari A. Medicinal plants. Tehran: Tehran University Press 1993; Vol. 3: pp. 511-4.Khorasani AMH. Makhzan-ol-Adviyeh. Tehran: Tehran University of Medical Sciences 2015.Elsagh M, Fartookzadeh MR, Kamalinejad M, et al. Efficacy of the Malva sylvestris L. flowers aqueous extract for functional constipation: A placebo-controlled trial. Complement Ther Clin Pract 2015; 21(2): 105-11. doi: 10.1016/j.ctcp.2015.02.003 PMID: 25801702Heidarpour M, Ennaifer E, Ahari H, et al. Histopathological changes induced by Hemiscorpius lepturus scorpion venom in mice. Toxicon 2012; 59(3): 373-8. doi: 10.1016/j.toxicon.2011.12.011 PMID: 22230352Pipelzadeh MH, Dezfulian AR, Jalali MT, Mansouri AK. In vitro and in vivo studies on some toxic effects of the venom from Hemiscorpious lepturus scorpion. Toxicon 2006; 48(1): 93-103. doi: 10.1016/j.toxicon.2006.04.017 PMID: 16777163Yazdkhasti M, Jalali MR, Khadjeh GH, Jafari H, Rezaie A. Cardiotoxic effects of Hemiscorpius lepturus scorpion venom fractions in rats. Iranian J Toxicol 2021; 15(1): 27-36. doi: 10.32598/IJT.15.1.691.1Borchani L, Sassi A, Ben Gharsa H, et al. The pathological effects of Heminecrolysin, a dermonecrotic toxin from Hemiscorpius lepturus scorpion venom are mediated through its lysophospholipase D activity. Toxicon 2013; 68: 30-9. doi: 10.1016/j.toxicon.2013.03.012 PMID: 23562369Najafi H, Yarijani MZ, Ashtiyani CS, et al. Protective effect of Malva sylvestris L. extract in ischemia-reperfusion induced acute kidney and remote liver injury. PLoS One 2017; 12(11): e0188270. doi: 10.1371/journal.pone.0188270 PMID: 29155898Benso B, Rosalen PL, Alencar SM, Murata RM. Malva sylvestris inhibits inflammatory response in oral human cells. An in vitro infection model. PLoS One 2015; 10(10): e0140331. doi: 10.1371/journal.pone.0140331 PMID: 26479870Dizaji R, Sharafi A, Pourahmad J, Abdollahifar MA, Vatanpour H, Hosseini MJ. Induction of two independent immunological cell death signaling following hemoglobinuria-induced acute kidney injury: In vivo study. Toxicon 2019; 163: 23-31. doi: 10.1016/j.toxicon.2019.03.011 PMID: 30890325Jurcau A, Ardelean IA. Molecular pathophysiological mechanisms of ischemia/reperfusion injuries after recanalization therapy for acute ischemic stroke. J Integr Neurosci 2021; 20(3): 727-44. doi: 10.31083/j.jin2003078 PMID: 34645107Kalogeris T, Baines CP, Krenz M, Korthuis RJ. Cell biology of ischemia/reperfusion injury. Int Rev Cell Mol Biol 2012; 298: 229-317. doi: 10.1016/B978-0-12-394309-5.00006-7 PMID: 22878108Borchani L, Sassi A, Shahbazzadeh D, et al. Heminecrolysin, the first hemolytic dermonecrotic toxin purified from scorpion venom. Toxicon 2011; 58(1): 130-9. doi: 10.1016/j.toxicon.2011.05.016 PMID: 21658401Torabi E, Behdani M, Chafi M, et al. Characteristics and lethality of a novel recombinant dermonecrotic venom phospholipase D from Hemiscorpius lepturus. Toxins 2017; 9(3): 102. doi: 10.3390/toxins9030102 PMID: 28335389