Current Pharmaceutical BiotechnologyCurrent Pharmaceutical BiotechnologyCurrent Pharmaceutical Biotechnology1389-20101873-4316Bentham Science64531010.2174/0113892010268142231226115140BiotechnologyResearch ArticleDiagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional BeadNogueiraLaisinfo@benthamscience.netMachadoJulianainfo@benthamscience.netDiasDanielinfo@benthamscience.netRodriguesRitainfo@benthamscience.netda PazMarianainfo@benthamscience.netTeixeira-NetoRafaelinfo@benthamscience.netReisAlexandreinfo@benthamscience.netGaldinoAlexsandroinfo@benthamscience.netSilvaEduardoinfo@benthamscience.netMouraHenriqueinfo@benthamscience.netKerHenriqueinfo@benthamscience.netRamosLuanainfo@benthamscience.netLaboratório de Biotecnologia de Microrganismos,, Federal University of São João del-ReiLaboratório de Biotecnologia de Microrganismos, Federal University of São João del-ReiLaboratório de Doenças Infecto-parasitárias, Universidade Federal de São João Del-ReiLaboratório de Nanobiotecnologia & Bioativos, Universidade Federal de São João Del-ReiLaboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas (NUPEB),, Universidade Federal de Ouro PretoLaboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Universidade Federal de Ouro Preto0109202425172290229907012025Copyright ©; 2024, Bentham Science Publishers2024Bentham Science Publishershttps://journals.eco-vector.com/1389-2010/article/view/645310

Background:Visceral leishmaniasis (VL) is a zoonotic disease, with dogs being the main reservoir of the Leishmania infantum parasite.

Objective:To develop a new flow cytometry test to diagnosis canine VL (CVL) diagnosis.

Methods:The current study addresses a new flow cytometry test using beads coupled to the multiepitope antigen rMELEISH.

Results:In the study set of samples a sensitivity (87.1%) and specificity (89.9%) was observed. Considering the dogs’ clinical status, 20/20 (100.0%) of the symptomatic sera tested positive, while 19/22 (86.4%) of the oligosymptomatic and 16/20 (80.0%) of asymptomatic were positive. In the non-infected control, all samples (0/30) tested as negative. In the cross-reaction control, the test was more efficient in dogs infected with L. braziliensis (2/10) and Trypanosoma cruzi (0/10), than those with Babesia canis (4/10) and Ehrlichia canis (4/10). Dogs immunized with different vaccines (Leishmune, Leish-Tec®, or LBSap) did not present serological reactivity.

Conclusion:The flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL.

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