Molecular genotypic detection of beta human papillomavirus DNA in the diagnosis of their association with certain skin epithelial neoplasias in immunocompromised and immunocompetent patients in the mode Real-Time

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access


Epidemiological and molecular biological data suggest that the beta human papillomavirus (HPV) can cause the development of a number of epithelial skin tumors, but this interconnection is currently not fully understood. Besides detection of beta HPV, it is necessary to measure and quantify the viral load, it is a new approach in the diagnosis of HPV infection. 52 patients were examined to identify the association of epithelial neoplasias beta human papilloma virus in immunocompromised and immunocompetent patients in the department of chronic hemodialysis and kidney transplantation of Moscow Regional Research and Clinical Institute. HPV detection was performed by polymerase chain reaction (PCR) with hybridization-fluorescence detection in real time (Real-Time). Amplification and detection were performed on the tool Rotor-Gene 3000 (“Corbett Research”, Australia). For quantification of beta HPV recombinant plasmid positive controls, as well as control plasmidfragment offi-globin human gene (FSIS CRIE Rospotrebnadzor) were used. In fibroepithelial polyp and in apparently healthy skin of immunosuppressive patients - renal transplant recipients (RPT) with a high frequency was detected beta HPV DNA - 64 and 54%, respectively, compared to healthy skin (47% of cases). In fibroepithelial polyp in 57% of immunosuppressive RPT mixed infection was revealed. Viral load in the fibroepithelial polyp was higher than that as in apparently normal skin and in donors skin. The high detection ofHPVDNA in RTP, as in the fibroepithelial polyp and in apparently healthy skin was found. Immunocompetent patients had high HPV DNA detection only in fibroepithelial polyp compared with apparently healthy skin.

Full Text

Restricted Access

About the authors

Lyubov V. Korneva

Moscow Regional Research and Clinical Institute

Moscow, 129110, Russian Federation
MD, PhD, assistant of Department of Dermato-venerology and Dermato-oncology, Postgraduate Training Faculty

E. S Snarskaya

I.M. Sechenov First Moscow State Medical University

Moscow, 119991, Russian Federation

E. K Nodelman

Central Research Institute of Epidemiology of Rospotrebnadzor of Russia

Moscow, 111123, Russian Federation


  1. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Human Papillomaviruses. Lyon; 2012. vol. 90.
  2. Harwood C.A., Surentheran T., McGregor J.M., Spink P.J., Leigh I.M., Breuer J., еt al. Human papillomavirus infection and non-melanoma skin cancer in immunosuppressed and immunocompetent individuals. J. Med. Virol. 2000; 61(3): 289-297.
  3. De Jong-Tieben L.M., Berkhout R.J., ter Schegget J., Vermeer B.J., de Fijter J.W., Bruijn J.A., еt al. The prevalence of human papillomavirus DNA in benign keratotic skin lesions of renal transplant recipients with and without a history of skin cancer is equally high: a clinical study to assess risk factors for keratotic skin lesions and skin cancer. Transplantation. 2000; 69(1): 44-9.
  4. Кладова А.Ю., Куевда Д.А., Молочков В.A., Шипулина О.Ю., Киселев В.И., Хлебникова А.Н. Встречаемость кожных типов вирусов папилломы человека в опухолях кожи. Альманах клинической медицины. 2006; 9: 44-50.
  5. Молочков В.А., Галил-Оглы Г.А., Сергеев Ю.В. Дерматоонкология. М.: Медицина; 2005: 34-40.
  6. Chen Q.P., Aw D.C. Epidemiology of skin diseases in renal transplant recipients in a tertiary hospital. Ann. Acad. Med. Singapore. 2010; 39(1): 904-5.
  7. Dianzani C., Calvieri S., Pierangeli A., Imperi M., Bucci M., Degener A.M. The detection of human papillomavirus DNA in skin tags. Br. J. Dermatol. 1998; 138(4): 649-51.
  8. Sallam M.A., Kamel M.M., Missiry A.G., Helal M.F. Detection of some types of human papillomaviruses in skin tags. Sci. J. Az. Med. Fac. (Girls). 2003; 24(1): 311-17.
  9. Pezeshkpoor F., Jafarian A.H., Ghazvini K., Yazdanpanah M.J., Sadeghian A., Esmaili H., еt al. An association of human papillomaviruses low risk and high risk subtypes with skin tag. Iran J. Basic Med. Sci. 2012; 15(3): 840-4.
  10. Корнева Л.В., Снарская Е.С., Молочков В.А., Полянская А.А. Генодиагностика ассоциации ДНК вирусов папилоломы человека рода beta с себорейным кератозом у иммуносупрессивных и иммунокомпетентных больных. Российский журнал кожных и венерических болезней. 2015; 18(2): 4-7.



Abstract - 42

PDF (Russian) - 1


Article Metrics

Metrics Loading ...



Copyright (c) 2016 Korneva L.V., Snarskaya E.S., Nodelman E.K.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies