Current Neurovascular Research

1567-20261875-5739

Bentham Science

644271

10.2174/0115672026294183240207115956

Medicine

Research Article

Electroacupuncture Alleviates Cerebral Ischemia-reperfusion Injury by Regulating the S1PR2/TLR4/NLRP3 Signaling Pathway via m6A Methylation of lncRNA H19

Zhang

Han-Rui

info@benthamscience.netMa

Gu-Quan

info@benthamscience.netLv

He-Qun

info@benthamscience.netFeng

Yao-Ting

info@benthamscience.netPeng

Yong-Jun

info@benthamscience.net

Department of Acupuncture and Rehabilitation, Affiliated Hospital of Nanjing University of Chinese MedicineDepartment of Acupuncture and Rehabilitation, The Affiliated Hospital of Nanjing University of Chinese Medicine

01012024

211647307012025

2024

Bentham Science Publishers

https://journals.eco-vector.com/1567-2026/article/view/644271

Electroacupuncture (EA) treatment plays a protective role in cerebral ischemiareperfusion (CIR) injury. However, the underlying molecular mechanism is still not fully elucidated.

Methods:All rats were randomly divided into five groups: the SHAM group, MCAO group, MCAO+EA (MEA) group, MCAO+METTL3 overexpression+EA (METTL3) group and MCAO+lncRNA H19 overexpression+EA (lncRNA H19) group. The middle cerebral artery occlusion (MCAO) rats were established to mimic CIR injury. The overexpression of lncRNA H19 and METTL3 was induced by stereotactic injection of lentiviruses into the rat lateral ventricles. The rats in the MEA, METTL3, and lncRNA H19 groups were treated with EA therapy on "Renzhong" (DU26) and "Baihui" (DU20) acupoints (3.85/6.25Hz; 1mA). Besides, the neurological deficit scoring, cerebral infarction area, pathological changes in brain tissue, total RNA m6A level, and the expression of METTL3, S1PR2, TLR4, NLRP3 and lncRNA H19 were detected in this experiment.

Results:EA improved the neurological deficit scoring, cerebral infarction area, and pathological injury in MCAO rats, while these beneficial effects of EA on CIR injury were attenuated by the overexpression of METTL3 or lncRNA H19. More importantly, EA down-regulated the total RNA m6A level and the expression of METTL3, S1PR2, TLR4, NLRP3 and lncRNA H19 in MCAO rats. Instead, the overexpression of METTL3 or lncRNA H19 was found to reverse the EA-induced down-regulation.

Conclusion:The findings indicated that EA might down-regulate the S1PR2/TLR4/NLRP3 signaling pathway via m6A methylation of lncRNA H19 to alleviate CIR injury. Our findings provide a new insight into the molecular mechanism of EA on CIR injury.

Electroacupuncturecerebral ischemia-reperfusionS1PR2/TLR4/NLRP3m6A methylationlncRNA H19METTL3.

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