Current HIV ResearchCurrent HIV ResearchCurrent HIV Research1570-162X1873-4251Bentham Science64415410.2174/011570162X304876240821062047MedicineResearch ArticleIdentification and Analysis of Differentially Expressed Genes Associated with Ferroptosis and HIV in PASMCs Based on BioinformaticsLuTonginfo@benthamscience.netGuoLinnainfo@benthamscience.netMaYonginfo@benthamscience.netYaoLijieinfo@benthamscience.netLiLiinfo@benthamscience.netBianWenshaninfo@benthamscience.netXiuMiaoinfo@benthamscience.netJiangYanginfo@benthamscience.netLiYongtaoinfo@benthamscience.netJinHaifenginfo@benthamscience.netCollege of Medical Technology, Qiqihar Medical UniversityDepartment of Anatomy, Qiqihar Medical University0105202422530831707012025Copyright ©; 2024, Bentham Science Publishers2024Bentham Science Publishershttps://journals.eco-vector.com/1570-162X/article/view/644154

Background:HIV-associated pulmonary arterial hypertension (HIV-PAH), a rare and fatal condition within the pulmonary arterial hypertension spectrum, is linked to HIV infection. While ferroptosis, an iron-dependent cell death form, is implicated in various lung diseases, its role in HIVPAH development remains unclear.

Methods:Leveraging Gene Expression Omnibus data, we identified differentially expressed genes (DEGs) in pulmonary arterial smooth muscle cells, including HIV-related DEGs (HIV-DEGs) and ferroptosis-related HIV-DEGs (FR-HIV-DEGs). PPI network analysis of FR-HIV-DEGs using CytoHubba in Cytoscape identified hub genes. We conducted functional and pathway enrichment analyses for FR-HIV-DEGs, HIV-DEGs, and hub genes. Diagnostic value assessment of hub genes utilized ROC curve analysis. Key genes were further screened, and external validation was performed. Additionally, we predicted a potential ceRNA regulatory network for key genes.

Results:1372 DEGs were found, of which 228 were HIV-DEGs, and 20 were FR-HIV-DEGs. TP53, IL6, PTGS2, IL1B (downregulated), and PPARG (upregulated) were the five hub genes that were screened. TP53, IL6, and IL1B act as ferroptosis drivers, PTGS2 as a ferroptosis marker, and PPARG as a ferroptosis inhibitor. Enrichment analysis indicated biological processes enriched in \"response to oxidative stress\" and pathways enriched in \"human cytomegalovirus infection.\" Key genes IL6 and PTGS2 exhibited strong predictive value via ROC curve analysis and external validation. The predicted ceRNA regulatory network identified miRNAs (has-mir-335-5p, has-mir-124-3p) targeting key genes and lncRNAs (XIST, NEAT1) targeting these miRNAs.

Conclusion:This study advances our understanding of potential mechanisms in HIV-PAH pathogenesis, emphasizing the involvement of ferroptosis. The findings offer valuable insights for future research in HIV-PAH.

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