Current Stem Cell Research & TherapyCurrent Stem Cell Research & TherapyCurrent Stem Cell Research & Therapy1574-888X2212-3946Bentham Science64558910.2174/011574888X274359231122064109MedicineResearch ArticleHuman Adipose-derived Stem Cells Upregulate IGF-1 and Alleviate Osteoarthritis in a Two-stage Rabbit Osteoarthritis ModelWangJuaninfo@benthamscience.netSuShiboinfo@benthamscience.netDongChuanminginfo@benthamscience.netFanQianginfo@benthamscience.netSunJishuinfo@benthamscience.netLiangSiqianginfo@benthamscience.netQinZuhuoinfo@benthamscience.netMaChuqinginfo@benthamscience.netJinJianfenginfo@benthamscience.netZhuHongweninfo@benthamscience.netJiangTongmenginfo@benthamscience.netXuJuninfo@benthamscience.netKey Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical UniversitySchool of Basic Medicine and Life Sciences, Hainan Medical UniversityDepartment of Anatomy, Medical College of Nantong UniversityOrthopedics Department, Qingdao Jimo District People's HospitalNeurosurgery Department,, Qingdao Jimo District People's Hospital, Zhongke Comprehensive Medical Transformation Center Research Institute (Hainan) Co., LtdThe Second Clinical College,, Hainan Medical UniversityDepartment of Biochemistry, School of Basic Medicine and Life Sciences,, Hainan Medical UniversityOrthopedics Department, Tianjin HospitalEngineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Functional Materials and Molecular Imaging, College of Emergency and Trauma,, Hainan Medical UniversityStem Cell Center, Shanghai East Hospital, School of Medicine, Tongji University0111202419111472148311012025Copyright ©; 2024, Bentham Science Publishers2024Bentham Science Publishershttps://journals.eco-vector.com/1574-888X/article/view/645589

Objective:In recent times, it has been recognized that mesenchymal stem cells (MSCs) possess the capability to address osteoarthritis (OA). The objective of this research was to examine the impact of injecting human adipose-derived stem cells (hADSCs) into a novel rabbit osteoarthritis model with dual damage.

Methods:The OA model was established surgically first by medial collateral ligament and anterior cruciate ligament transection and medial meniscectomy, then by articular cartilage full-thickness defect. Enhanced Green Fluorescence Protein expressing lentivirus FG12 was used to label hADSCs, which were then injected into the knee joints. Every single rabbit was sacrificed after 4 and 8 weeks following the surgical procedure. Macroscopic examination, immunohistochemistry staining, magnetic resonance imaging, qRT-PCR, and ELISA analysis were utilized for the assessments.

Results:After 4 and 8 weeks, the injection of hADSCs resulted in reduced cartilage loss, minimal fissures and cracks, and a decrease in the volume of joint effusion and cartilage defect as measured by MRI. Moreover, the application of ELISA and qRT-PCR techniques revealed that the administration of hADSCs resulted in an elevation in the IGF-1 concentration.

Conclusions:Based on our findings, it can be inferred that the transplantation of hADSCs facilitates the healing of articular cartilage in the osteoarthritis model of rabbits with double damage. The upregulated IGF-1 may play a crucial part in the process of cartilage repair using hADSCs. The use of hADSC transplantation could potentially be appropriate for clinical implementation in managing osteoarthritis.

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