Study of coordinated regulation of expression of tnfa and matrix metalloproteinases MMP8 and MMP9 in periodontal tissue in health and pathology


A qPCR method combined with reverse transcription applicable for obtaining reproducible results of mRNA analysis of human periodontal swabs is established. A positive correlation between mRNA of TNFa, MMP8, MMP9 in healthy periodontal tissue is demonstrated. This correlation is not found in patients with chronic periodontitis. A mechanism of TNFa-dependent induction of MMP8 and MMP9 induction is found to be pronounced in men than in women.

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About the authors

Oksana Aleksandrovna Zorina

Sechenov First Moscow State Medical University; Central Research Institute of Dental and Maxillofacial Surgery of Ministry of Health of the Russian Federation

199911, Moscow
doctor of medicine, professor, I.M. Sechenov First Moscow State Medical University, Central Research Institute of Dental and Maxillofacial Surgery of Ministry of Health of the Russian Federation

N. K Aymadinova

Sechenov First Moscow State Medical University

199911, Moscow

O. A Boriskina

Sechenov First Moscow State Medical University; Central Research Institute of Dental and Maxillofacial Surgery of Ministry of Health of the Russian Federation

199911, Moscow

A. B Shevelev

Chumakov institute of poliomyelitis and viral encephalitides

142782, Moscow


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