Study of coordinated regulation of expression of tnfa and matrix metalloproteinases MMP8 and MMP9 in periodontal tissue in health and pathology

Abstract


A qPCR method combined with reverse transcription applicable for obtaining reproducible results of mRNA analysis of human periodontal swabs is established. A positive correlation between mRNA of TNFa, MMP8, MMP9 in healthy periodontal tissue is demonstrated. This correlation is not found in patients with chronic periodontitis. A mechanism of TNFa-dependent induction of MMP8 and MMP9 induction is found to be pronounced in men than in women.

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About the authors

Oksana Aleksandrovna Zorina

Sechenov First Moscow State Medical University; Central Research Institute of Dental and Maxillofacial Surgery of Ministry of Health of the Russian Federation

Email: zorina-cniis@yandex.ru
199911, Moscow
doctor of medicine, professor, I.M. Sechenov First Moscow State Medical University, Central Research Institute of Dental and Maxillofacial Surgery of Ministry of Health of the Russian Federation

N. K Aymadinova

Sechenov First Moscow State Medical University

199911, Moscow

O. A Boriskina

Sechenov First Moscow State Medical University; Central Research Institute of Dental and Maxillofacial Surgery of Ministry of Health of the Russian Federation

199911, Moscow

A. B Shevelev

Chumakov institute of poliomyelitis and viral encephalitides

142782, Moscow

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