Characteristics of the pathogenic potential of Staphylococcus aureus strains isolated from prostate secretions in chronic bacterial prostatitis

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Abstract

Aim. To analyze genes that determine pathogenicity factors in staphylococcal strains isolated from prostatic secretions in chronic bacterial prostatitis.

Materials and methods. Staphylococci were isolated from the prostate secretion of men of reproductive age (20-45 years) with chronic bacterial prostatitis using a conventional bacteriological method. The type of staphylococci was determined by MALDI-TOF mass spectrometry. The virulence genes of staphylococci were determined by PCR and sequencing.

Results. The isolated strains were found to have a wide distribution of genetic determinants of pathogenicity, namely adhesion, colonization, invasion, and immune resistance. It was shown that most genes are associated with evasion of the host immune response during infection, which contributes to the chronic course of bacterial prostatitis.

Conclusion. It is advisable to further study the genetic determinants of pathogenicity/virulence of Staphylococcus aureus to assess the severity and duration of chronic bacterial prostatitis.

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About the authors

Mikhail D. Kuzmin

Institute of Cellular and Intracellular Symbiosis Ural Branch of the Russian Academy of Sciences – a separate structural subdivision of the OFRC Ural Branch of the Russian Academy of Sciences

Author for correspondence.
Email: mdk-49@mail.ru
ORCID iD: 0000-0002-6349-6053

Dr. Med. Sci., leading researcher laboratories for studying the mechanisms of formation of human microbial biocenoses, urologist of the highest qualification category, consultant of GAUZ "OOKB", Honored Doctor of the Russian Federation and the Republic of Mordovia

Russian Federation, Orenburg

Olga L. Kartashova

Institute of Cellular and Intracellular Symbiosis Ural Branch of the Russian Academy of Sciences – a separate structural subdivision of the OFRC Ural Branch of the Russian Academy of Sciences

Email: labpersist@mail.ru
ORCID iD: 0000-0002-1487-7546
Scopus Author ID: 7003322797

Dr. Biol. Sci., associate professor, leading researcher of laboratories of persistence and symbiosis of microorganisms

Russian Federation, Orenburg

Olga A. Kapustina

Institute of Cellular and Intracellular Symbiosis Ural Branch of the Russian Academy of Sciences – a separate structural subdivision of the OFRC Ural Branch of the Russian Academy of Sciences

Email: olga25mikro@mail.ru
ORCID iD: 0000-0001-9944-3095
Scopus Author ID: 57196410629

PhD in Biol. Sci., senior scientific of laboratories of persistence and symbiosis of microorganisms

Russian Federation, Orenburg

Tatiana M. Pashkova

Institute of Cellular and Intracellular Symbiosis Ural Branch of the Russian Academy of Sciences – a separate structural subdivision of the OFRC Ural Branch of the Russian Academy of Sciences

Email: pashkova070782@mail.ru
ORCID iD: 0000-0001-8075-8249
Scopus Author ID: 57189696210

Dr. Biol. Sci., leading researcher of laboratories of persistence and symbiosis of microorganisms

Russian Federation, Orenburg

Lidiya P. Popova

Institute of Cellular and Intracellular Symbiosis Ural Branch of the Russian Academy of Sciences – a separate structural subdivision of the OFRC Ural Branch of the Russian Academy of Sciences

Email: labpersist@mail.ru
ORCID iD: 0000-0002-3766-6020
Scopus Author ID: 56946322300

PhD in Med. Sci., senior scientific of laboratories of persistence and symbiosis of microorganisms

Russian Federation, Orenburg

Viktor A. Gritsenko

Institute of Cellular and Intracellular Symbiosis Ural Branch of the Russian Academy of Sciences – a separate structural subdivision of the OFRC Ural Branch of the Russian Academy of Sciences

Email: vag59@mail.ru
ORCID iD: 0000-0002-2086-5170
Scopus Author ID: 7101892654

Doctor of Medical Sciences, Professor, Chief Researcher of laboratories of persistence and symbiosis of microorganisms

Russian Federation, Orenburg

References

  1. Ibishev H.S., Kogan M.I., Magomedov R.G., Krajnij P.A. Modern view on the pathogenetic bases of chronic recurrent bacterial prostatitis. Effective pharmacotherapy. Epidemiology and infection. 2017;2:6–10. Russian (Ибишев Х.С., Коган М.И., Магомедов Р.Г., Крайний П.А. Современный взгляд на патогенетические основы хронического рецидивирующего бактериального простатита. Эффективная фармакотерапия. Эпидемиология и инфекция. 2017;2:6–10).
  2. Galimov SH.N., Galimova E.F., Mochalov K.S., Enikeev D.A., Borcova YU.L., Muratov E.M., Galimov K.SH. Features of immunological parameters in chronic bacterial prostatitis. Medical Bulletin of Bashkortostan. 2020;15(85):5–8. Russian (Галимов Ш.Н., Галимова Э.Ф., Мочалов К.С., Еникеев Д.А., Борцова Ю.Л., Муратов Э.М., Галимов К.Ш. Особенности иммунологических показателей при хроническом бактериальном простатите. Медицинский вестник Башкортостана. 2020;15(85):5–8). https://medvestb.elpub.ru/jour/article/view/508/508.
  3. Buharin O.V., Kuz’min M.D., Perunova N.B., Ivanova E.V., Bekpergenova A.V., Bondarenko T.A. Characteristics of microbiota and cytokine profile of spermoplasm in patients with chronic bacterial prostatitis. Urologiia. 2020;5:67–72. Russian (Бухарин О.В., Кузьмин М.Д., Перунова Н.Б., Иванова Е.В., Бекпергенова А.В., Бондаренко Т.А. Характеристика микробиоты и цитокинового профиля спермоплазмы у больных хроническим бактериальным простатитом. Урология. 2020;5:67–72). doi: 10.18565/urology.2020.5.67-72.
  4. Kartashova O.L., Pashinina O.A., Pashkova T.M., Gricenko V.A., Popova L.P. Pathogenicity factors and antibiotic resistance of Enterococcus faecalis strains isolated from prostatic secretion in men with chronic bacterial prostatitis. Pacific Medical Journal. 2024;2:60–64. Russian (Карташова О.Л., Пашинина О.А., Пашкова Т.М., Гриценко В.А., Попова Л.П. Факторы патогенности и антибиотикорезистентность штаммов Enterococcus faecalis, выделенных из секрета предстательной железы у мужчин с хроническим бактериальным простатитом. Тихоокеанский медицинский журнал. 2024;2:60–64). doi: 10.34215/1609-1175-2024-2-60-64.
  5. Kogan M.I., Ibishev H.S., Naboka YU.L., Gudima I.A., Ismailov R.S. Extended bacteriological examination as a key to revising antibacterial therapy for chronic bacterial prostatitis. Urologiia. 2023;1:5–11. Russian (Коган М.И., Ибишев Х.С., Набока Ю.Л., Гудима И.А., Исмаилов Р.С. Расширенное бактериологическое исследование как ключ к пересмотру антибактериальной терапии хронического бактериального простатита. Урология. 2023;1:5–11). doi: 10.18565/ urology.2023.1.5-11.
  6. Tunik T.V., Ivanova E.I., Grigorova E.V., Voropaeva N.M., Vishnevskaya V.A. Spectrum of opportunistic microflora isolated from prostatic secretion in chronic bacterial prostatitis. Acta Biomedica Scientifica. 2017;2:70–73. Russian (Туник Т.В., Иванова Е.И., Григорова Е.В., Воропаева Н.М., Вишневская В.А. Спектр представителей условно-патогенной микрофлоры, выделенной из секрета простаты при хроническом бактериальном простатите. Acta Biomedica Scientifica. 2017;2:70–73). doi: 10.12737/article_5a3a0dd8cfc440.60849200.
  7. Naboka YU.L., Kogan M.I., CHernickaya M.L., Gudima I.A., Ibishev H.S., Ferzauli A.H. Microbial spectrum of prostate secretion and persistence factors of bacteria found in chronic bacterial prostatitis. Byulleten’ Orenburgskogo nauchnogo centra Uro RAN. 2012;3:2–6. Russian (Набока Ю.Л., Коган М.И., Черницкая М.Л., Гудима И.А. Ибишев Х.С., Ферзаули А.Х. Микробный спектр секрета предстательной железы и факторы персистенции бактерий, обнаруженных при хроническом бактериальном простатите. Бюллетень Оренбургского научного центра Уро РАН. 2012;3:2–6). http://elmag.uran.ru:9673/magazine/Numbers/2012-3%20/Articles/10Naboka.pdf.
  8. Kartashova O.L., Pashinina O.A., Pashkova T.M., Gricenko V.A., Mihajlenko S.V. Bioprofiles of various types of staphylococci isolated from prostate secretions in men with chronic bacterial prostatitis. Tihookeanskij medicinskij zhurnal. 2023;1:5–9. Russian (Карташова О.Л., Пашинина О.А., Пашкова Т.М., Гриценко В.А., Михайленко С.В. Биопрофили стафилококков разных видов, выделенных из секрета предстательной железы у мужчин с хроническим бактериальным простатитом. Тихоокеанский медицинский журнал. 2023;1:70–74). doi: 10.34215/1609-1175-2023-1-70-74.
  9. Bozhedomov V.A. Chronic prostatitis: a new treatment paradigm. Urologiia. 2016;3:78–90. Russian (Божедомов В.А. Хронический простатит: новая парадигма лечения. Урология. 2016;3:78–90). https://urologyjournal.ru/ru/archive/article/33331.
  10. Kartashova O.L., Pashkova T.M., Tyapaeva YA.V., Belozerceva YU.P., Kurlaev P.P., Mavzyutov A.R., Gricenko V.A. Staphylococcus aureus: genetic diversity taking into account the source of isolation. Bulletin of the Orenburg Scientific Center of the Ural Branch of the Russian Academy of Sciences. 2018;3:10. Russian (Карташова О.Л., Пашкова Т.М., Тяпаева Я.В., Белозерцева Ю.П., Курлаев П.П., Мавзютов А.Р., Гриценко В.А. Staphylococcus aureus: генетическое разнообразие с учетом источника выделения. Бюллетень Оренбургского научного центра УрО РАН. 2018;3:10). http://elmag.uran.ru:9673/magazine/Numbers/2018-3/Articles/KOL-2018-3.pdf.
  11. Litwin M.S., McNaughton-Collins M., Fowler F.J. Jr. et al. The National Institutes of Health chronic prostatitis symptom index: development and validation of a new outcome measure. Chronic Prostatitis Collaborative Research Network. Journal Urology. 1999;162:369–375. doi: 10.1016/s0022-5347(05)68562-х.
  12. Rosen R.C., Cappelleri J.C., Smith M.D., Lipsky J., Peña B.M. Development and evaluation of an abridged, 5-item version of the International Index of Erectile Function (IIEF-5) as a diagnostic tool for erectile dysfunction. International Journal of Impotence Research. 1999;11(6):319–326. doi: 10.1038/sj.ijir.3900472.
  13. GOST R 52379-2005 Good clinical practice. Moscow: Standartinform. 2006; 33 p. Russian. (ГОСТ Р 52379-2005 Надлежащая клиническая практика. Москва: Стандартинформ. 2006; 33 с).
  14. Eisenbeis J., Saffarzadeh M., Peisker H. et al. The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation. Frontiers in Cellular and Infection Microbiology. 2018;8:235. doi: 10.3389/fcimb.2018.00235.
  15. Boero E., Cruz A.R., Pansegrau W. et al. Natural Human Immunity Against Staphylococcal Protein A Relies on Effector Functions Triggered by IgG3. Frontiers in Immunology. 2022;13:834711. doi: 10.3389/fimmu.2022.834711.
  16. Dryla A., Gelbmann D., von Gabain A., Nagy E. Identification of a novel iron regulated staphylococcal surface protein with haptoglobin-haemoglobin binding activity. Molecular Microbiology. 2003;49(1):37–53 doi: 10.1046/j.1365-2958.2003.03542.x.
  17. Zhao M., Qin C., Li L. et al. Conjugation of Synthetic Trisaccharide of Staphylococcus aureus Type 8 Capsular Polysaccharide Elicits Antibodies Recognizing Intact Bacterium. Frontiers in Chemistry. 2020; 8: 258. doi: 10.3389/fchem.2020.00258.
  18. Sivakumar R., Pranav P.S., Isloor M.A.S., Rajendhran J., Hegde N.R. Genome sequencing and comparative genomic analysis of bovine mastitis-associated Staphylococcus aureus strains from India. BMC Genomics. 2023;24:44 doi: 10.1186/s12864-022-09090-7.
  19. Tyurin Yu.A., Fassahov R.S. Patent of the Russian Federation No. 2532225 Method for identification of genes of staphylococcal exotoxins with high structural homology to superantigens by the method of multiplex polymerase chain reaction and a test system for its implementation. Published on 27.10.2014. Bulletin No. 30. Russian. (Тюрин Ю.А., Фассахов Р.С. Патент РФ № 2532225 Способ идентификации генов стафилококковых экзотоксинов, с высокой структурной гомологией с суперантигенами методом мультиплексной полимеразной цепной реакции и тест-система для его осуществления. Опубликовано 27.10.2014. Бюл. № 30).
  20. Johri A.V., Johri P., Hoyle N. et al. Case report: Successful treatment of recurrent E. coli infection with bacteriophage therapy for patient suffering from chronic bacterial prostatitis. Frontiers in Pharmacology. 2023;14:1243824. doi: 10.3389/fphar.2023.1243824.
  21. Deryabin D.G. Staphylococci: ecology and pathogenicity. Ekaterinburg, 2001. 238 p. Russian. (Дерябин Д.Г. Стафилококки: экология и патогенность. Екатеринбург, 2001. 238 с).
  22. Kartashova O.L., Boklin A.K., Kirgizova S.B. Characteristics of biological properties of pathogens causing chronic sinusitis. Russian Rhinology. 2005;2:71. Russian (Карташова О.Л., Боклин А.К., Киргизова С.Б. Характеристика биологических свойств возбудителей хронических синуситов. Российская ринология. 2005;2:71).
  23. Buharin O.V., Kartashova O.L., Kurlaev P.P. Using Staphylococcus Persistence Factors to Predict the Course of Purulent-Inflammatory Diseases. Journal of Microbiology, Epidemiology and Immunobiology. 1998;5:27–30. Russian (Бухарин О.В., Карташова О.Л., Курлаев П.П. и др. Использование факторов персистенции стафилококков для прогнозирования течения гнойно-воспалительных заболеваний. Журнал микробиологии, эпидемиологии и иммунобиологии. 1998;5:27–30).
  24. Kartashova O.L., Gandybin E.A., Utkina T.M., Abramzon O.M., Kirgizova S.B. Biological properties of microorganisms in predicting the course of venous trophic ulcers of the lower extremities. Journal of Microbiology, Epidemiology and Immunobiology. 2009;4:111–114. Russian (Карташова О.Л., Гандыбин Е.А., Уткина Т.М., Абрамзон О.М., Киргизова С.Б. Биологические свойства микроорганизмов в прогнозировании течения венозно-трофических язв нижних конечностей. Журнал микробиологии, эпидемиологии и имммунобиологии. 2009;4:111–114).
  25. Buharin O.V., From persistence to symbiosis. Journal of microbiology, epidemiology and immunobiology. 2012;4:4–9. Russian (Бухарин О.В. От персистенции к симбиозу. Журнал микробиологии, эпидемиологии и иммунобиологии. 2012;4:4–9).
  26. Paharik A.E., Horswill A.R. The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response. Microbiology Spectrum. 2016;4:10. doi: 1128/microbiolspec.vmbf-0022-2015.
  27. Ganske A., Busch L.M., Hentschker C. et al. Exploring the targetome of IsrR, an iron-regulated sRNA controlling the synthesis of iron-containing proteins in Staphylococcus aureus. Frontiers in Microbiology. 2024;15:1439352. doi: 10.3389/fmicb.2024.1439352.

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