SPERM DNA FRAGMENTATION: ASSOCIATION WITH SEMEN PARAMETERS IN YOUNG MEN


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Аннотация

Aim. Abnormal sperm DNA integrity is an important risk factor for male infertility. The aim of this work was to examine sperm DNA fragmentation in a cohort of young male volunteers (n=111; age 21.0±0.2 years) from the general population and establish the association between the level of sperm DNA fragmentation and sperm functional parameters. Materials and methods. Sperm DNA fragmentation index (DFI) was determined by SCSA (sperm chromatin structure assay) using flow cytometry. Standard semen parameters (concentration, motility, and morphology) were evaluated according to the WHO guidelines (2010). Results and conclusions. In the study cohort, 79.0%, 12.4% and 8.6% of men had normal (DFI<15%), borderline (15≤DFI<27%) and high (DFI≥27%) levels of fragmentation, respectively. Men with impaired spermatogenesis had greater IDF values (14.53±1.43%) than men with normal semen parameters (8.88+0.77%, p<0.05). There was a statistically significant negative correlation between IFD and ejaculate concentration (r=-0.21, p<0.05), fractions ofmobile (r=-0.41, p<0.05) and morphologically normal sperm (r=- 0.34, p<0.05). Testing sperm DNA fragmentation using SCSA technique can be employed in epidemiological studies of male fertility.

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Авторлар туралы

L. Osadchuk

Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS

Email: losadch@bionet.nsc.ru
Dr.Bio.Sci., Professor, Leading Research Fellow at the Department of Human Genetics Novosibirsk

D. Tataru

Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS; Krasnoyarsk Center for Reproductive Medicine

Email: tataru_dasha@inbox.ru
Novosibirsk; Krasnoyarsk

N. Kuznetsova

Ergin Medical center

Email: ergin1@dscom.ru
Kemerovo

M. Kleshev

Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS

Email: max82cll@bionet.nsc.ru
Novosibirsk

E. Markova

Krasnoyarsk Center for Reproductive Medicine

Email: markova88@mail.ru
Krasnoyarsk

A. Svetlakov

Krasnoyarsk Center for Reproductive Medicine

Email: krasivf@kcrm.ru
Krasnoyarsk

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