The quantification of sulphated glycosaminoglycans in rat urine in experimental hemorrhagic cystitis


如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

The paper presents the study of the excretion of sulfated glycosaminoglycans (GAG) in the urine of rats in experimental hemorrhagic cystitis induced by cyclophosphamide and treated with glycosaminoglycan replacement therapy. Rats were given intraperitoneal injections of cyclophosphamide at a dose of 100 mg per 1 kg body weight and subsequently treated with intragastric administration of the combined preparation of glycosaminoglycans containing glucosamine hydrochloride and chondroitin sulfate at a dose of 10 and 100 mg per 1 kg of body weight. Within 24 or 72 hours after cystitis induction there was a statistically significant increase in urinary GAG excretion. The study also found a decrease (from 1.34 to 1.22 mg/dL) in urinary GAG within 0 to 72 hours following induction of acute cystitis without glycosaminoglycan therapy. In the subchronic model of inflammation in the bladder, upon repeated administration of low doses of cyclophosphamide (50 mg/kg), decrease in urinary GAG within 0 to 72 hours (1,32±0,13 mg/dL) as well as increased excretion after 96 hours at a concentration of 2,29±0,13 mg/L after initiation cystitis were found.

全文:

受限制的访问

作者简介

V. Sobolev

FSUE «Research institute of hygiene, occupational pathology and human ecology» of FMBA of Russia

PhD in Veterinary Sciences., Senior Researcher of Laboratory of Molecular Toxicology and Experimental Therapeutics

V. Shmurak

FSUE «Research institute of hygiene, occupational pathology and human ecology» of FMBA of Russia

参考

  1. Akcay T., Konukoglu D. Glycosaminoglycans excretion in interstitial cystitis. Int. Urol. Nephrol. 1999;31(4):431-435.
  2. Lokeshwar V.B., Selzer M.G, Cerwinka W.H. Urinary uronate and sulfated glycosaminoglycan levels: markers for interstitial cystitis severity. J. Urol. 2005;174:344-349.
  3. Wei D.C., Politano V.A., Selzer M.G., Lokeshwar V.B. The association of elevated urinary total to sulfated glycosaminoglycan ratio and high molecular mass hyaluronic acid with interstitial cystitis. J. Urol. 2000;163(5):1577-1583.
  4. Siracusano S., Cucchi A., Ciciliato S., Lampropoulou N., Vittur F. Urinary levels of glycosaminoglycans in patients with idiopathic detrusor overactivity. Int. Urogynecol. J. 2009;20:1477-1480.
  5. Maccari F, Buzzega D., Galeotti F, Volpi N. Fine structural characterization of chondroitin sulfate in urine of bladder pain syndrome subjects. Int. Urogynecol. J. 2011;22:1581-1586.
  6. Soler R., Bruschini H., Martins J.R., Dreyfuss J.L., Camara N.O., Alves M.T., Leite K.R., Truzzi J.C., Nader H.B., Srougi M., Ortiz V. Urinary glycosaminoglycans as biomarker for urothelial injury: is it possible to discriminate damage from recovery? Urology. 2008;72(4):937-942.
  7. Parsons C.L. Prevention of urinary tract infection by the exogenous glycosaminoglycan sodium pentosanpolysulfate. J. Urol. 1982;127:167-169.
  8. Theodaris T.C., Sant G.R. (2001) New agents for the medical treatment of interstitional cystitis Exp. Opin. Invest Drugs 10 (3): 251-246.
  9. Кудрявцев Ю.В., Кирпатовский В.И., Перепанова Т.С. Хазан П.Л. Применение стабилизатора гликозаминогликанов - гиалуроната цинка, при экспериментальном моделировании острого бактериального и интерстициального цистита и др. Экспериментальная и клиническая урология. 2011;1:39-44.
  10. Grant D.K. Measurement of urinary glycosaminoglicans in dogs. Diss. MSc degree Virginia Polytechnic institute, 2003.
  11. Savolainen H. A sensitive method for the analysis of urinary proteoglycans. Biochem. Int. 1992:28:475-479.
  12. Mitsuhashi H., Tsukada Y., Ono K. Urine glycosaminoglycans and heparin sulfate excretions in adult patients with glomerular duseases. Clin. Nephrol. 1993;39:231-238.
  13. De Jong J.G., Heijs W.M., Wevers R.A. Mucopolysaccharidoses screening: dimethylmethylene blue versus alcian blue. Ann. Clin. Biochem. 1994;31:267-271.
  14. Kery V., Orlovska M., Stancikova M. Urinary glycosaminoglycan excretion in rheumatic diseases. Clinical Chemistry. 1992; 38:841-846.
  15. Budak Y.U., Huysal K., Guray A. Urinary glycosaminoglycan excretion in patients with primary nocturnal enuresis. Italian Journal of Pediatrics. 2010;36:13.
  16. Pereira M.L., Pizauro J.M.Jr, Carvalho M.B. Adapted colorimetric method for measurement of feline urinary glycosaminoglycans. Comparative Clinical Pathology. 2014;23(2):323-326.
  17. Erickson D.R., Ordille S., Martin A., Bhavanandan V.P. Urinary chondroitine sulfates, heparin sulfate and total sulfated glycosaminoglycans in interstitional cystitis. J. Urol. 1997; 157:61-64.
  18. Panchaphanpong J., Asawakarn T., Pusoonthornthum R. Effects of oral administration of N-acetyl-d-glucosamine on plasma and urine concentrations of glycosaminoglycans in cats with idiopathic cystitis. Am. J. Vet. Res. 2011;72:843-850.
  19. Chen Y.T., Yang C.C., Sun C.K., Chiang H.J., Chen Y.L., Sung P.H., Zhen Y.Y., Huang T.H., Chang C.L., Chen H.H., Chang H.W., Yip H.K. Extracorporeal shock wave therapy ameliorates cyclophosphamide- induced rat acute interstitial cystitis though inhibiting inflammation and oxidative stress-in vitro and in vivo experiment studies. Am. J. Transl. Res. 2014;6(6):631-648.
  20. Ribeiro R.A., Lima-Junior R.C.P., Leite C.V.G., etal. Chemotherapy- induced hemorrhagic cystitis: pathogenesis, pharmacological approaches and new insights. J. Exp. Int. Med. 2012;2(2):95-112.

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Bionika Media, 2015
##common.cookie##