ASSOCIATION OF POLYMORPHISM OF GSTT1 AND GSTM1 GENES WITH INFERTILITY IN MEN


如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

Aim. To identify the association between homozygous deletion genotypes of glutathione transferase genes GSTT1 (glutathione transferase theta 1), GSTM1 (glutathione S-transferase mu1) and infertility in Russian menю. Materials and methods. The article presents a comparative analysis of the incidence of homozygous deletion genotypes of glutathione transferase genes GSTM1 and GSTT1 in Russian men with and without infertility. The study group comprised 160 infertile Russian men of reproductive age (mean age 30.2±3.6 years.) The infertility diagnosis was verified according to the WHO guidelines. The control group comprised 104 healthy Russian volunteers (mean age 31.3±5.4 years.) Molecular genetic detection of GSTM1 and GSTT1 deletion polymorphisms was performed using PCR. The genomic DNA for the study was extracted from whole blood samples. Results. The study and control group differed significantly in incidence of GSTM1 (p=0.043) and GSTT1 (p=0.008) deletion polymorphisms. The probability of detecting «zero» genotypes of the GSTT1 and GSTM1 genes in infertile men was 2.5 (p<0.05) and 1.7 times higher (p<0.05), respectively, than in fertile men. Conclusions. Therefore, the study findings allow us to conclude that the deletion genotypes of GSTM1 and GSTT1 are associated with infertility in Russian men. Molecular genetic analysis of deletion polymorphism of glutathione transferase genes can be recommended for a comprehensive examination of infertile men.

全文:

受限制的访问

作者简介

N. Kurashova

Research Center for Family Health and Human Reproduction

Email: iphr@sbamsr.irk.ru
Ph.D. (bio.sci), Senior Researcher at the Laboratory of Pathophysiology Irkutsk, Russia

E. Belyaeva

Research Center for Family Health and Human Reproduction

Email: iphr@sbamsr.irk.ru
Ph.D. (bio.sci), Researcher at the Laboratory of Personalized Medicine Irkutsk, Russia

O. Ershova

Research Center for Family Health and Human Reproduction

Email: iphr@sbamsr.irk.ru
Ph.D. (bio.sci), Researcher at the Laboratory of Personalized Medicine Irkutsk, Russia

B. Dashiev

Republican Perinatal Center of Minzdrav of BR

Email: iphr@sbamsr.irk.ru
Ph.D., Urologist-Andrologist Ulan-Ude, Russia

T. Bairova

Research Center for Family Health and Human Reproduction

Email: iphr@sbamsr.irk.ru
Dr.Med.Sci., Deputy Director for Science, Head of the Laboratory of Personalized Medicine Irkutsk, Russia

L. Kolesnikova

Research Center for Family Health and Human Reproduction

Email: iphr@sbamsr.irk.ru
Prof., Academician of RAS, Research Supervisor Irkutsk, Russia

参考

  1. Kolesnikova L.I., Kolesnikov S.I., Kurashova N.A., Bairova T.A. Causes and Factors of Male Infertility. Vestnik Rossijskoj akademii medicinskih nauk. 2015;70(5):579-584.
  2. Agarwal A., Mulgund A., Hamada A., Chyatte M. A unique view on male infertility around the globe. Reproductive Biology and Endocrinology. 2015;13:37. doi: 10.1186/s12958-015-0032-1.
  3. EAU Guidelines on Male Infertility 2010. http://www.uroweb.org/ professional-resources/guidelines/. Russian (Мужское бесплодие: рекомендации Европейской ассоциации урологов. 2010 http:// www.uroweb.org/professional-resources/guidelines/).
  4. Finotti A.C., Costa E., Silva R.C., Bordin B.M., Silva C.T., Moura K.K. Glutathione S-transferase M1 and T1 polymorphism in men with idiopathic infertility. Genet Mol Res. 2009;8(3): 1093-98. doi: 10.4238/vol8-3gmr642.
  5. Poliakova I.S., Churnosov M.I., Pachomov S.P., Orlova V.S. Molecular and genetic mechanisms of xenobiotic biotransformation. auchnye vedomosti Belgorodskogo gosudarstvennogo universiteta. Seriya: Medicina. Farmaciya. 2011;16(111):223-228.
  6. Hayes J.D., Flanagan J.U., Jowsey I.R. Glutathione transferases. Annual Review of Pharmacology and Toxicology. 2005;45:51-88.
  7. Tew K.D., Townsend D.M. Glutathione-s-transferases as determinants of cell survival and death. Antioxidants & Redox Signaling. 2012;17:1728-1737.
  8. Wu B., Dong D. Human cytosolic glutathione transferases: structure, function, and drug discovery. Trends in Pharmacological Sciences. 2012;33:656-668.
  9. The genetic passport - the basis of individual and predictive medicine/edited by V.S. Baranov. SPt.: Publishing N-L, 2009.
  10. Shenin V.A., Labygina A.V., Nesterova D.I., Urybin I.IU., Suturina L.V., Lazareva L.M., Ermolova E.V., Beliaeva E.V. The gene polymorphism of detoxification system in women with genital endometriosis and infertility. Acta Biomedica Scientifica. 2009;S2:74-75
  11. Xu X.B., Liu S.R., Ying H.Q., A Z.C. Null genotype of GSTM1 and GSTT1 may contribute to susceptibility to male infertility with impaired spermatogenesis in Chinese population. Biomarkers. 2013;18(2):151-154. doi: 10.3109/1354750X.2012.755221.
  12. Glantz S.A. Biomedical Statistics. Moscow: Practice, 1999.
  13. Rebrova O. Iu. Statistical analysis of medical data. Moscow: Media Sfera, 2002.
  14. Tirumala Vani G., Mukesh N., Siva Prasad B., Rama Devi P., Hema Prasad M., Usha Rani P., Pardhanandana Reddy P. Role of glutathione S-transferase Mu-1 (GSTM1) polymorphism in oligospermic infertile males. Andrologia. 2010;42(4):213-217. doi: 10.1111/j.1439-0272.2009.00971.x.
  15. Aydos S.E., Taspinar M., Sunguroglu A., Aydos K. Association of CYP1A1 and glutathione S-transferase polymorphisms with male factor infertility. Fertil Steril. 2009;92(2):541-547. Doi: 10.1016/j. fertnstert.2008.07.017.
  16. Wu W., Lu J., Tang Q., Zhang S., Yuan B., Li J., Di Wu, Sun H., Lu C., Xia Y., Chen D., Sha J., Wang X. GSTM1 and GSTT1 null polymorphisms and male infertility risk: an updated meta-analysis encompassing 6934 subjects. Sci Rep. 2013;3:2258. Doi:10.1038/ srep02258.
  17. Aydemir B., Onaran I., Kiziler A.R., Alici B., Akyolcu M.C. Increased oxidative damage of sperm and seminal plasma in men with idiopathic infertility is higher in patients with glutathione S-transferase Mu-1 null genotype. Asian J. Androl. 2007;9(1):108-115. Doi:10.1111/ j.1745-7262.2007.00237.x.
  18. Wu Q., Xing J., Xue W., Sun J., Wang X., Jin X. Influence of polymorphism of glutathione S-transferase T1 on Chinese infertile patients with varicocele. Fertil Steril. 2009;91(3):960-962. doi: 10.1016/j.fertnstert.2007.08.061.
  19. Glybochko P.V., Alyaev Yu.G., Chalyy M.E., Usacheva O.A. Influence of the glutatione S-transferases T1 and M1 gene polymorphisms on androgenic status and semen quality after surgical treatment of varicocele. Andrology and Genital Surgery. 2013; 1 (14) :23-26
  20. Santovito A., Cervella P., Burgarello C., Bigatti M.P., Sella G., Delpero M. Analysis of glutathione S-transferase M1 and glutathione S-transferase T1 gene polymorphisms suggests agerelated relationships in a northern Italian population. Arch Toxicol. 2008;82(12):903-07. doi: 10.1007/s00204-008-0316-8.
  21. Zendeh-Boodi Z., Saadat M. Genetic polymorphism of GSTT1 may be under natural selection in a population chronically exposed to natural sour gas. Mol Biol Rep. 2008;35(4):673-76. Doi:10.1007/ s11033-007-9139-6.

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Bionika Media, 2017
##common.cookie##