Motorized sample positioning system equipped with an optical module

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Abstract

Scanning capillary microscopy is becoming increasingly important in the study of living objects, as it enables direct, non-contact visualization of cell and tissue morphology in a natural aqueous environment. Nanometer-scale, non-contact imaging and functional analysis of fragile biological samples are becoming a cornerstone of modern biomedical research. The unique capabilities of SCM for live-cell imaging, combined with multiparametric analysis and nanoscale delivery, make this method indispensable for life sciences, electrochemistry, biotechnology, and medicine. In this paper, we will discuss in detail the implementation of a mechanical system capillary microscope.

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About the authors

A. I. Akhmetova

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-5115-8030

Physical Department, Cand. of Sci. (Physics and Mathematics), Senior Researcher, Leading Specialist

Russian Federation, Moscow; Moscow

D. I. Yaminsky

Lomonosov Moscow State University

Email: yaminsky@nanoscopy.ru
ORCID iD: 0009-0009-6370-7496

Physical Department, Post Graduate

Russian Federation, Moscow

A. P. Iakovlev

MISIS University of Science and Technology

Email: yaminsky@nanoscopy.ru
ORCID iD: 0009-0004-6832-6426

Post Graduate, Scientific Project Engineer

Russian Federation, Moscow

V. S. Kolmogorov

MISIS University of Science and Technology

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-7135-8910

Scientific Project Engineer

Russian Federation, Moscow

A. N. Vaneev

MISIS University of Science and Technology

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8201-8498

Cand. of Sci. (Chemistry), Scientific Project Consultant

Russian Federation, Moscow

A. O. Prelovskaya

MISIS University of Science and Technology

Email: yaminsky@nanoscopy.ru
ORCID iD: 0009-0005-0486-3211

Scientific Project Engineer

Russian Federation, Moscow

P. V. Gorelkin

JSC "IKAPPIC"

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-4860-9013

Cand. of Sci. (Physics and Mathematics), General Manager

Russian Federation, Moscow

A. S. Erofeev

MISIS University of Science and Technology

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-6631-821X

Doct. of Sci. (Physics and Mathematics), Head of Laboratory

Russian Federation, Moscow

I. V. Yaminsky

Lomonosov Moscow State University; Advanced Technologies Center

Author for correspondence.
Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8731-3947

Physical Department, Doct. of Sci. (Physics and Mathematics), Prof., Director General

Russian Federation, Moscow; Moscow

References

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  2. Akhmetova A.I., Terentyev A.D., Fedoseyev A.I., Yaminsky D.I., Yaminsky I.V. Scanning capillary microscopy for biological applications. NANOINDUSTRY. 2024. Vol. 17. No. 6. https://doi.org/10.22184/1993-8578.2024.17.6.364.370
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  7. Основной государственный регистрационный номер: 1217700310101, идентификационный номер 9722004219, https://reestr.digital.gov.ru/reestr/1968404/?sphrase_id=7059264
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Supplementary files

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2. Fig.1. FemtoScan X Ion scanning capillary microscope on an inverted Nikon Ti-U optical microscope with a 40x objective lens

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3. Fig.2. Compact version of the FemtoScan X Ion scanning capillary microscope with a motorized sample positioning system, camera, and autofocus

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4. Fig.3. Model of a motorized sample positioning system equipped with an optical module: 1 – mounting beam, 2 – Piezo manipulators for high-precision movement, 3 – positioner for coarse movement of the nanocapillary, 4 – two-coordinate automated sample movement platform in the XY plane, 5 – optical module for automatic focusing and recognition of individual cells

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5. Fig.4. Automated stage for coarse movement of the sample in the XY plane

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6. Fig.5. Model of a positioner for coarse movement of a nanocapillary in a vertical plane

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7. Fig.6. Mounting layout of the piezo manipulator to the coarse positioning stage

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8. Fig.7. Method of attaching a piezo manipulator to a positioning device for coarse movement: 1 – positioner for coarse movement, 2 – transitional mount, 3 – Piezo manipulator for moving the probe along the vertical axis, 4 – mounting beam, 5 – Piezo manipulator for moving the probe along the XY axis

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9. Fig.8. Optical module equipped with an automatic focusing function: 1 – table for mounting a positioner for coarse movement, 2 – lens location, autofocus module, illuminator, 3 – mount for digital camera, 4 – transitional mount for optical table

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10. Fig.9. Optical image of individual cells on a substrate with cell boundaries processed using an AI model

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11. Fig.10. Optical (left) and fluorescent (right) images of melanocyte cells after local intracellular delivery of fluorescently labeled dextran

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Copyright (c) 2025 Akhmetova A.I., Yaminsky D.I., Iakovlev A.P., Kolmogorov V.S., Vaneev A.N., Prelovskaya A.O., Gorelkin P.V., Erofeev A.S., Yaminsky I.V.