Diffraction grating as a means of metrological support of microscopy

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Abstract

Visualization of biomedical samples in their natural environment at micro- and nanoscale is crucial for studying fundamental principles of biosystems functioning with complex interactions. The study of dynamic biological processes requires a microscopic system with multiple measurement capabilities, high spatial and temporal resolution, versatile visualization environments and local manipulation options. Scanning capillary microscopy and microlens microscopy are promising tools for these tasks, but correct operation of either technique is impossible without metrological support. This paper demonstrates the possibility of using a diffraction grating sample for these purposes.

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About the authors

A. I. Akhmetova

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-5115-8030

Cand. of Sci. (Physics and Mathematics), Researcher

Russian Federation, Moscow; Moscow

A. D. Terentev

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0009-0009-1528-5284

Master, Progammer

Russian Federation, Moscow; Moscow

S. A. Senotrusova

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0003-0960-8920

Master, Leading Engineer

Russian Federation, Moscow; Moscow

D. I. Yaminsky

Lomonosov Moscow State University

Email: yaminsky@nanoscopy.ru
ORCID iD: 0009-0009-6370-7496

Post Graduate

Russian Federation, Moscow

V. V. Popov

Lomonosov Moscow State University

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0003-1191-3860

Senior Researcher

Russian Federation, Moscow

I. V. Yaminsky

Lomonosov Moscow State University; Advanced Technologies Center

Author for correspondence.
Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8731-3947

Doct. of Sci. (Physics and Mathematics), Prof., Director

Russian Federation, Moscow; Moscow

References

  1. Senotrusova S.A., Akhmetova A.I., Yaminsky I.V. Super-resolution ability of microlenses for the study of biological objects. NANOINDUSTRY. 2023. Vol. 16. No. 3–4. PP. 168–176. https://doi.org/10.22184/1993-8578.2023.16. 3-4.168.176
  2. Yaminsky I.V., Akhmetova A.I., Senotrusova S.A., Wang Z., Bing Y., Lukyanchuk B.S., Barmina E., Simakin A.V., Shafeev G.A. A new solution for bionanoscopy based on optical microlens technology. NANOINDUSTRY. 2021. Vol. 14. No. 5. PP. 292–297. https://doi.org/10.22184/1993-8578.2021.14.5.292.297

Supplementary files

Supplementary Files
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2. Fig.1. Magnified images of diffraction grating topography through BaTiO3 microspheres in silicone oil. The diameter of the left microlens is 90 μm and the right microlens is 73 μm

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3. Fig.2. Magnified image of the grating through polymethylacrylate microspheres. The average diameter of the spheres is ~10 µm

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4. Fig.3. 2D and 3D topography of the relief diffraction grating. According to the cross-section data: the band width is slightly more than 800 nm, the height of the bands is about 300 nm

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5. Fig.4. SCM image of the diffraction grating and measurement of characteristic parameters of fringes: period and width

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6. Fig.5. 3D diffraction grating profile and measurement of characteristic fringe parameters after microscope calibration

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Copyright (c) 2024 Akhmetova A.I., Terentev A.D., Senotrusova S.A., Yaminsky D.I., Popov V.V., Yaminsky I.V.