Three-dimensional visualization of viruses, bacteria and cells in the educational process

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Abstract

Scanning probe microscopy has proven itself as a unique device for solving problems in the field of virology, cell biology, biomedicine, regenerative medicine, materials science and microelectronics. This data can be used in scientific research, for teachers of natural sciences at school it is useful to expand the horizon of the educational program and show schoolchildren not only the world in an optical microscope or in formulas on the board. Atoms, molecules, proteins, viruses, bacteria and cells can be seen or even touched with an atomic force microscope, and this is a look at nanoobjects from a completely different angle.

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About the authors

A. I. Akhmetova

Lomonosov Moscow State University; Advanced Technologies Center

Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0002-5115-8030

Researcher, Leading Specialist

Lomonosov Moscow State University, Physical Department

Russian Federation, Moscow; Moscow

I. V. Yaminsky

Lomonosov Moscow State University; Advanced Technologies Center

Author for correspondence.
Email: yaminsky@nanoscopy.ru
ORCID iD: 0000-0001-8731-3947

Doct. of Sci. (Physics and Mathematics), Prof., Director

Lomonosov Moscow State University, Physical Department

Russian Federation, Moscow; Moscow

References

  1. Akhmetova A.I., Sovetnikov T.O., Obolenskaya L.N., Yaminsky I.V. FemtoScan Online in Scientific and Educational Activities: Count, Measure, and Visualize. NANOINDUSTRY. 2024. Vol. 17. No. 6. PP. 392–398. https://doi.org/10.22184/1993-8578.2024.17.6.392.398
  2. Vointseva I.I., Gembitskiy P.A. Polyguanidines – disinfectants and multifunctional additives in composite materials. Moscow, LKM-press, 2009. P. 303.
  3. Sovetnikov T.O., Akhmetova A.I., Gukasov V.M., Evtushenko G.S., Rybakov Y.L., Yaminskii I.V. Scanning probe microscopy in assessing blood cells roughness. Bio-Medical Engineering. 2023. https://doi.org/10.1007/s10527-023-10253-3
  4. Trukhova A.A., Akhmetova A.I., Yaminsky I.V. Visualization of erythrocytes by atomic force microscopy. NANOINDUSTRY. 2023. Vol. 16. No. 3–4. https://doi.org/10.22184/1993-8578.2023.16.3-4.180.184
  5. Sinitsyna O.V., Akhmetova A.I., Yaminsky I.V. Atomic force microscopy of erythrocytes: new diagnostic possibilities. Medicine and High Technologies. 2022. Vol. 1. PP. 9–12. https://doi.org/ 10.34219/2306-3645-2022-12-1-9-12

Supplementary files

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2. Fig.1. a – 2D; b – 3D-AFM image of tick-borne encephalitis virus on graphite surface

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3. Fig.2. Viral particles of tick-borne encephalitis on the graphite surface

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4. Fig.3. Image of tick-borne encephalitis virus obtained with different values of applied force. Top left 2D view, top right 3D image, white-red dashed line shows the cross-section. The height difference was almost 50 nm between the 10 and 20 nN force scans

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5. Fig.4. Pseudomonas aeruginosa. Reference sample. Top left 2D view, top right 3D view. According to the cross-sectional data, the cell height is about 190 nm

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6. Fig.5. Pseudomonas aeruginosa after exposure to OHMG. Top left 2D view, top right 3D view. According to the cross-section data, the height is about 230 nm

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7. Fig.6. Blood cell imaging. 2D view, 3D image and cross section. According to the cross-sectional data, the diameter of an erythrocyte is about 9.5 µm

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Copyright (c) 2024 Akhmetova A.I., Yaminsky I.V.