Journal of Volgograd State Medical UniversityJournal of Volgograd State Medical University1994-94801994-9499Volgograd State Medical University11932210.19163/1994-9480-2018-4(68)-97-101Research ArticleDEVELOPMENT OF THE TECHNIQUE FOR DETERMINATION OF NATURAL IMPURITIES IN THE SUBSTANCE OF A NEW CONNECTION OF GK-2 PEPTIDE STRUCTUREGrushevskayaL. Notopharm@mail.ruSergeevaM. S-GaevayaL. M-DudenkovaM. E-DenisenkoE. D-AvdyuninaN. I-MinaevS. V-SazonovaN. M-FSBSI «Research Institute of Pharmacology named after V. V. Zakusov» RAMS150420181549710118122022Copyright © 2018, Grushevskaya L.N., Sergeeva M.S., Gaevaya L.M., Dudenkova M.E., Denisenko E.D., Avdyunina N.I., Minaev S.V., Sazonova N.M.2018The study is devoted to the development of related substances determination technique in the original dipeptide, which is fourth loop of nerve growth factor mimetic - GK-2, and was carried out using methods of thin-layer chromatography and high-performance liquid chromatography with spectrophotometric detection (HPLC and TLC). The technique for determining of the related impurities content includes two stages: analysis by thin-layer chromatography and analysis by high-performance liquid chromatography. The developed technique allows detecting impurities in the substance in the content of 0,1 % and more.GK-2substanceanalysisrelated substancesforeign substancestechniquehigh-performance liquid chromatographythin-layer chromatographyГК-2субстанцияанализродственные примесипосторонние примесиметодикавысокоэффективная жидкостная хроматографиятонкослойная хроматография[Середенин С.Б., Гудашева Т.А. Дипептидные миметики нейротрофинов NGF и BDNF. Патент РФ № 2410392. Изобр. № 3, 2011.][Середенин С.Б., Гудашева Т.А. Создание фармакологически активной малой молекулы, обладающей свойствами фактора роста нервов // Журн. неврологии и психиатрии. - № 6. - 2015. - C. 63-70.][Gudasheva T.A., Povarnina P.Yu., Antipova T.A., Seredenin S.B. A novel dimeric dipeptide mimetic of the nerve growth factor exhibits pharmacological effects upon systemic administration and has no side effects accompanying the neurotrophin treatment // Neuroscience & Medicine. - № 5. - 2014. - P. 101-108.]