Journal of Volgograd State Medical UniversityJournal of Volgograd State Medical University1994-94801994-9499Volgograd State Medical University11942410.19163/1994-9480-2020-2(74)-61-66Research ArticleA MODIFIED METHOD FOR STUDYING THE GLYCOXIDATION REACTIONLitvinovR. Alitvinov.volggmu@mail.ruKosolapovV. A-MuravyovaE. A-SkachkoI. V.-ShamshinaD. D-FSBEI HE «Volgograd State Medical University» of Public Health Ministry of the Russian Federation15022020172616618122022Copyright © 2020, Litvinov R.A., Kosolapov V.A., Muravyova E.A., Skachko I.V., Shamshina D.D.2020The article considers a particular model of the glycation reaction, called glycoxidation and characterized by a significant involvement of oxidative reactions and the participation of transition metals. The enhancement of oxidative processes, which makes it possible to identify the reaction as glyoxidation, was realized by introducing copper (II) cations (CuSO4 * 5H2O) into the reaction medium in a form accessible for interaction with the reaction participants. The main reagents in the glycoxidation reaction, in addition to CuSO4*5H2O are bovine serum albumin (1 g/l), glucose (0,5 M), HEPES buffer solution (24 g/l) dissolved in deionized water. The conditions have been selected under which the glycoxidation reaction linearly depends on the concentration of copper (II), which makes the cation activity a sensitive target when monitoring the reaction. The dependence of the reaction on the concentration of CuSO4 * 5H2O under the presented experimental conditions has been confirmed experimentally and mathematically. An analytical range of concentrations of CuSO4*5H2O has been established, in which a linear dependence of AGE formation on concentration is observed. For the concentration of CuSO4*5H2O, which produces the greatest potentiating effect on the AGE formation (10 mg/l), the procedure was validated by the indices of internal laboratory reproducibility and convergence. The technique is of interest for investigation of the antiglycoxidative activity of novel compoundsCuSO4*5H2OHEPESGlycoxidationCuSO4*5H2OHEPESbovine serum albuminglucoseglycation end products (AGE)гликоксидациябычий сывороточный альбуминглюкозаконечные продукты гликирования (КПГ)[Соколова Е.В., Хохлачева Е.А., Шамшина Д.Д. и др. Активность новых соединений на основе азолоазина на моделях оценки антигликирующего действия и ингибирования ДПП-4 // Вестник Волгоградского государственного медицинского университета. - 2019. - № 1 (69). -С. 79-82.][Шамшина Д.Д., Литвинов Р.А. Валидация методики медь-зависимого аутоокисления аскорбиновой кислоты как способа оценки активности соединений, связывающих d-элементы // Вестник Волгоградского государственного медицинского университета. - 2018. - № 1 (65). -С. 115-117.][Cho S.J., Roman G., Yeboah F., Konishi Y. The road to advanced glycation end products: a mechanistic perspective // CurrMedChem. - 2007. - No. 14 (15). - P. 1653-1671.][Conover W. J. Practical Nonparametric Statistics. -3rd Edition. - 1999. - 592 p.][Diabetes Atlas. International Diabetes Federation. -9rd Edition. - 2019. - 29 p.][Eid S., Sas K.M., Abcouwer S.F., et al. New insights into the mechanisms of diabetic complications: role of lipids and lipid metabolism // Diabetologia. - 2019. - Vol. 62(9). -P. 1539-1549. - doi: 10.1007/s00125-019-4959-1.][Savateev K.V., Fedotov V.V., Butorin I., et al. Nitro-thiadiazolo[3,2-a]pyrimidines as promising antiglycating agents // European Journal of Medicinal Chemistry. - 2020. -Vol. 185. - Art. No. 111808.][Serban I.A., Condac E., Costache M., Dinischiotu A. The relationship between ages, Cu2+ and crosslinking of collagen // Revue Roumainede Chimie. - 2009. - Vol. 54 (1). -P. 93-101.][Spasov A.A., Babkov D.A., Sysoeva V.A., et al. 6-nitroazolo[1,5-a]pyrimidin-7(4h)-onesasantidiabeticagents // ArchivderPharmazie. - 2017. - Vol. 350, no. 12. - Art. No. e1700226.][Spasov A.A., Brel A.K., Litvinov R.A., et al. Evaluation of N-hydroxy-, N-metoxy-, and N-acetoxybenzoyl- substituted derivatives of thymine and uracil as new substances for prevention and treatment of long-term complications of diabetes mellitus // Russian Journal of Bioorganic Chemistry. -2018. - Vol. 44, no. 6. - С. 769-777.][Ramirez Segovia A.S., Wrobel K., Acevedo Aguilar F.J., et al. Effectof Cu (II) on in vitro glycation of human serum albumin by methylglyoxal: a LC-MS-based proteomic approach // Metallomics. - 2017. - Vol. 9 (2). -P. 132-140. - doi: 10.1039/c6mt00235h.][Yamagishi S., Maeda S., Matsui T., et al. Role of advanced glycation end products (AGEs) and oxidative stress in vascular complications in diabetes // Biochim Biophys Acta. - 2012. - No. 1820 (5). - P. 663-671.][Zieman S., Kass D. Advanced glycation end product cross-linking: pathophysiologic role and therapeutic target in cardiovascular disease // CongestHeartFail. - 2004. -Vol. 10 (3). - P. 144-149.]