Journal of Volgograd State Medical UniversityJournal of Volgograd State Medical University1994-94801994-9499Volgograd State Medical University11964710.19163/1994-9480-2021-2(78)-128-131Research ArticleСHARACTERISTICS OF THE EXPRESSION OF ENDOTHELIAL NO-SYNTHASE IN THE DENTATE GYRUS OF RATS WHEN MODELING A DEPRESSIVE STATE AND THE POSSIBILITY OF PHARMACOLOGICAL CORRECTION WITH PHENIBUTSmirnovA. V-EkovaM. Rmaria.ekova@mail.ruTyurenkovI. N-VolotovaE. V-FSBEI HE «Volgograd State Medical University» of the Ministry of Healthcare of the Russian FederationSBI «Volgograd Medical Scientific Center»1502202118212813119122022Copyright © 2021, Smirnov A.V., Ekova M.R., Tyurenkov I.N., Volotova E.V.2021Depression is one of the leading causes of declining working capacity in the population and remains one of the most common mental disorders in the world in all age groups. The features of the expression of endothelial NO-synthase in the dentate gyrus in mature rats were assessed when simulating a depressive state, as well as the possibility of pharmacological correction of the revealed changes with phenibut. Evaluation of changes in the level of expression of immunoreactive material in the dentate gyrus was determined in the granular and subgranular layers by assessing the intensity of staining in points from 0 to 3. In rats with an experimental depression, compared with the control group, there was a decrease in the degree of expression of immunoreactive material in the dentate gyrus. The use of phenibut contributed to the correction of the studied signs.eNOSdentate gyrusdepressionimmunohistochemistryeNOSphenibutзубчатая извилинадепрессияиммуногистохимияфенибут[Chen J., Zacharek A., Zhang C., et al. Endothelial nitric oxide synthase regulates brain-derived neurotrophic factor expression and neurogenesis after stroke in mice. J Neurosci, 2005, no. 25 (9), pp. 2366-2375. DOI: 10.1523/JNEUROSCI.5071-04.2005.][Cheung A., Newland P.L., Zaben M., et al. Intracellular nitric oxide mediates neuroproliferative effect of neuropeptide Y on postnatal hippocampal precursor cells. Journal of Biological Chemistry, 2012, no. 287 (24), pp. 20187-20196. DOI: 10.1074/jbc.m112.346783.][Koch S.BJ., Morey R.A., Roelofs K. The role of the dentate gyrus in stress-related disorders. Molecular Psychiatry, 2020, no. 25, pp. 1361-1363. DOI: 10.1038/s41380-019-0572-4][Miller B.R., Hen R. The current state of the neurogenic theory of depression and anxiety. Current Opinion in Neurobiology, 2015, no. 30, pp. 51-58. DOI:10.1016/j.conb.2014.08.012.][Ni S., Huang H., He D., et al. Adeno-associated virus-mediated over-expression of CREB-regulated transcription coactivator 1 in the hippocampal dentate gyrus ameliorates lipopolysaccharide-induced depression-like behaviour in mice. Journal of Neurochemistry, 2019, no. 1 (149), pp. 111-125. DOI: 10.1111/jnc.14670.][Reif A., Schmitt A., Fritzen S., et al. Differential effect of endothelial nitric oxide synthase (NOS-III) on the regulation of adult neurogenesis and behaviour. European Journal of Neuroscience, 2004, no. 20 (4), pp. 885-895. DOI: 10.1111/j.1460-9568.2004.03559.x.][Smirnov A.V., Tyurenkov I.N., Shmidt M.V., et al. Effect of combined stress on morphological changes and expression of NO synthases in rat ventral hippocampus. Bull. Exp. Biol. Med., 2015, no. 1 (160), pp. 96-99.][Steinert J.R., Chernova T., Forsythe I.D. Nitric oxide signaling in brain function, dysfunction, and dementia. The Neuroscientist, 2010, no. 16 (4), pp. 435-452. DOI: 10.1177/ 1073858410366481.][Trujillo C.A., Negraes P.D., Schwindt T.T., et al. Kinin-B2 receptor activity determines the differentiation fate of neural stem cells. Journal of Biological Chemistry, 2012, no. 287 (53), pp. 44046-44061. DOI: 10.1074/jbc.m112.407197.][Tyurenkov I.N., Bagmetova V.V., Chernysheva Y.V., Borodin D.D. A depressive state in rats in chronic combined st ress induced by combined stresses of different modalities. Neuroscience and behavioral physiology, 2015, no. 5 (45), pp. 542-549.]