Efficacy of Superlymph in preparation for in vitro fertilization in patients with thin endometrium

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Abstract

Objective: Evaluation of the effectiveness of topical therapy using a complex of natural antimicrobial peptides and cytokines (Superlymph) in patients with thin endometrium (TE) and reproductive dysfunction prior to in vitro fertilization and embryo transfer (IVF).

Materials and methods: Ninety patients with TE and diagnosis N97.2: Female infertility of uterine origin (oocyte implantation defect) were included. Groups I (n=45) received Superlymph, Group II (n=45) received autologous platelet-rich plasma (PRP) injections into the endometrial submucosal layer, and Group III (n=45) included women with normal endometrial thickness (male factor infertility). Research methods included clinical, laboratory (smear microscopy and real-time PCR of vaginal contents), biochemical, functional (pelvic ultrasound, Doppler ultrasound), morphological, and immunohistochemical (IHC) tests (CD138 determination).

Results: After treatment, M-echo in the second phase of the cycle increased from 5.8 and 5.4 mm in Groups I and II, respectively, to 7.3 mm in both groups; sclerosis of the spiral arteries of the endometrium significantly decreased, and the endometrial structure corresponded to the day of the menstrual cycle; laboratory testing showed no local inflammation. The pregnancy rate in Group I was 42.2%, while in Group II it was 37.7%, with an absolute difference in rates of 4.5% in favor of Superlymph. The odds of pregnancy and live birth were comparable between the groups (OR=1.12, 95% CI: 0.67–1.86, p=0.83 and OR=1.23, 95% CI: 0.67–2.25, p=0.65, respectively). Pregnancy outcomes were also comparable and did not differ statistically significantly from the control group.

Conclusion: Therapy using the Superlymph complex in patients with TE and infertility before IVF is no less effective than PRP therapy.

About the authors

Galina B. Dikke

F.I. Inozemtsev Academy of Medical Education

Author for correspondence.
Email: galadikke@yandex.ru
ORCID iD: 0000-0001-9524-8962

Dr. Sci. (Med.), Professor, Department of Obstetrics and Gynecology with a Course in Reproductive Medicine

Russian Federation, St. Petersburg

Anton A. Sukhanov

Perinatal Center, Tyumen; Tyumen State Medical University

Email: saa2505anton@yandex.ru
ORCID iD: 0000-0001-9092-9136

Dr. Sci. (Med.), Head of the Department of Gynecology, Associate Professor, Department of Obstetrics and Gynecology

Russian Federation, Tyumen; Tyumen

Anastasia V. Pesotskaya

Altai Regional Clinical Perinatal Center

Email: pesokpesotskaya@yandex.ru
ORCID iD: 0000-0002-4625-0916

Head of the Pathology Laboratory with the Immunohistochemistry Department

Russian Federation, Barnaul

Irina I. Kukarskaya

Perinatal Center, Tyumen; Tyumen State Medical University

Email: galadikke@yandex.ru
ORCID iD: 0000-0002-8275-3553

Dr. Sci. (Med.), Professor, Department of Obstetrics, Gynecology, and Resuscitation with the Course in Clinical Laboratory Diagnostics, Institute of Continuous Professional Development, Chief Physician, Chief Specialist in Obstetrics and Gynecology, Healthcare Department of the Tyumen Region

Russian Federation, Tyumen; Tyumen

Natalya V. Shilova

National Research Nuclear University MEPhI

Email: nvshilova@gmail.com
ORCID iD: 0000-0001-6734-0147

Cand. Sci. (Med.), Associate Professor, Department of Industrial Pharmacy

Russian Federation, Moscow

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