Clinical digitalization of the ultrastructural state of the podocyte in the process of infectious nephropathy implementation (pilot study)

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective. Evaluation of the feasibility of clinical digitalization of podocyte ultrastructural state using positron emission tomography/computed tomography (PET/CT) indicator of standardized 18F-FDG uptake by renal parenchymal and transmission electron microscopy data in a case of infectious tubulointerstitial nephritis.

Materials and methods. A pilot study of digital analysis of renal 18F-FDG PET/CT scans and podocyte ultrastructural assessment using transmission electron microscopy at 10,000x magnification was conducted.

Results. A pilot study demonstrated that digital PET/CT imaging of the standardized 18F-FDG uptake value (SUVmax) by renal parenchyma correlated with laboratory manifestations of infectious nephropathy and the main dimensional characteristics of the podocyte body, which correlated with its visual ultrastructural changes.

Conclusion. The feasibility of clinical digitalization of nephropathies is demonstrated using infectious tubulointerstitial nephritis as an example.

About the authors

Boris A. Berdichevsky

Tyumen State Medical University

Author for correspondence.
Email: doktor_bba@mail.ru
ORCID iD: 0000-0002-9414-8510

Dr.Sci. (Med.), Professor, Department of Pediatric Surgery with a Course in Urology and Andrology

Russian Federation, Tyumen

Tatyana N. Vasilkova

Tyumen State Medical University

Email: vasilkova@tyumsmu.ru
ORCID iD: 0000-0003-4753-6630

Dr.Sci. (Med.), Professor, Chief External Expert in Therapy of the Ural Federal District, Head of the Department of Faculty Therapy

Russian Federation, Tyumen

Grigory V. Zubik

Tyumen State Medical University; Radiology Center, Tyumen Regional Clinical Medical Center «Medical City»

Email: grisha.zubik@mail.ru
ORCID iD: 0009-0005-1333-2922

Research Intern, Institute of Clinical Medicine, Tyumen State Medical University

Russian Federation, Tyumen; Tyumen

Daniil A. Uchaev

South Ural State University, National Research University

Email: uchaevda@susu.ru
ORCID iD: 0000-0002-8623-4769

Leading Researcher, Department of Electron Microscopy and X-ray Analysis Methods, Research and Education Center «Nanotechnology»

Russian Federation, Chelyabinsk

Elizaveta S. Grimak

Tyumen State Medical University

Email: liza.grimak@mail.ru
ORCID iD: 0009-0006-1240-1620

Student, Institute of Clinical Medicine

Russian Federation, Tyumen

Aleksandra A. Egorova

Tyumen State Medical University

Email: ealexandraa12@mail.ru
ORCID iD: 0009-0005-2234-9662

Student, Institute of Clinical Medicine

Russian Federation, Tyumen

Malvina K. Biybulatova

Tyumen State Medical University

Email: malvina.biybulatova@mail.ru
ORCID iD: 0009-0002-7420-0702

Student, Institute of Clinical Medicine

Russian Federation, Tyumen

References

  1. Котенко О.Н., Черненко О.В., Гурьянова Л.Д. Свидетельство о государственной регистрации программы для ЭВМ № 2023681384 Российская Федерация. Оценка качества жизни, связанного со здоровьем, пациентов с хронической болезнью почек «НЕФРО-ТЕСТ КЖ»: № 2023680860: заявл. 12.10.2023: опубл. 12.10.2023. [Kotenko O.N., Chernenko O.V., Guryanova L.D. Certificate of State Registration of the Computer Program No. 2023681384, Russian Federation. Assessment of the Health-Related Quality of Life of Patients with Chronic Kidney Disease «NEPHO-TEST QL»: № 2023680860: applied for on 12.10.2023: published on 12.10.2023.].
  2. Бердичевский Б.А., Василькова Т.Н., Зубик Г.В. Свидетельство о государственной регистрации программы для ЭВМ № 2025690098. Российская Федерация. Программа блочной 3D-ультраструктурной визуализации подоцита с увеличением в 15 000 раз при реализации хронической болезни почек на примере тубулоинтерстициального нефрита инфекционного генеза. Заявл. 24.10.2025; опубл. 05.11.2025. [Berdichevsky B.A., Vasylkova T.N., Zubik G.V. et al. Certificate of state registration of software № 2025690098. Russian Federation. Block 3D ultra-structural visualization program of podocyte with 15000x magnification for chronic kidney disease demonstration on the example of infectious tubulointerstitial nephritis. Filed 24 Oct 2025; published 5 Nov 2025.].
  3. Смирнов А.В., Наточин Ю.В. Нефрология: фундаментальная и клиническая. Нефрология. 2019;23(4):9–26. [Smirnov A.V., Natochin Yu.V. Nephrology: Fundamentals and Clinical. Nefrology. 2019;23(4):9–26.]. doi: 10.24884/1561-6274-2019-23-4-9-26.
  4. Madrazo-Ibarra A., Vaitla P. Histology, Nephron. 2023 Feb 17. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan. [PMID: 32119298].
  5. Husain S. Role of podocyte in kidney disease. Front Biosci (Landmark Ed). 2024;29(7):250. doi: 10.31083/j.fbl2907250. [PMID: 39082335].
  6. Pollak M.R., Quaggin S.E., Hoenig M.P., Dworkin L.D. The glomerulus: the sphere of influence. Clin J Am Soc Nephrol. 2014;9(8):1461–9. doi: 10.2215/CJN.09400913. [Epub 2014 May 29, PMID: 24875196, PMCID: PMC4123398].
  7. Audzeyenka I., Bierżyńska A., Lay A.C. Podocyte Bioenergetics in the Development of Diabetic Nephropathy: The Role of Mitochondria. Endocrinology. 2022;163(1):bqab234. https://doi.org/10.1210/endocr/bqab234.
  8. Dondi F., Pisani A.R., Lucarelli N.M. et al. Correlation between Kidney Uptake at [18F]FDG PET/CT and Renal Function. J Pers Med. 2023;14(1):40. doi: 10.3390/jpm14010040. [PMID: 38248741, PMCID: PMC10817585].
  9. Бердичевский Б.А., Бердичевский В.Б., Сапоженкова Е.В. и др. ПЭТ/КТ молекулярно-клеточная визуализация острого пиелонефрита в диагностике скрытых причин лихорадки неясного генеза. Урология. 2024;4:75–80. [Berdichevsky B.A., Berdichevsky V.B., Sapozhenkova E.V. et al. PET/CT molecular-cellular visualization of acute pyelonephritis in the diagnosis of hidden causes of fever of unknown origin. Urology. 2024;4:75–80.]. doi: 10.18565/urology.2024.4.75-80. EDN: LUUGOL.
  10. Moghrabi S., Abdulkadir A.S., Al-Hajaj N. et al. A new era for PET/CT: applications in non-tumorous renal pathologies. J Clin Med. 2024;13(16):4632. doi: 10.3390/jcm1316463.
  11. Бердичевский Б.А., Бердичевский В.Б., Зубик Г.В. Свидетельство о государственной регистрации программы для ЭВМ № 2025663706. Российская Федерация. Компьютерная программа визуальной и блочной 3D-ультраструктурной оценки вероятности воспалительных изменений в нефроне по результатам ПЭТ/КТ всего тела 18F-ФДГ глюкозой. Заявл. 29.05.2025; опубл. 29.05.2025. [Berdichevsky B.A., Berdichevsky V.B., Zubik G.V. Certificate of state registration of software № 2025663706. Russian Federation. Computer program for visual and block 3D ultra-structural assessment of the probability of inflammatory changes in the nephron based on whole-body 18F-FDG PET/CT glucose uptake. Filed 29 May 2025; published 29 May 2025.].
  12. Бердичевский Б.А., Бердичевский В.Б., Зубик Г.В. и др. Свидетельство о государственной регистрации программы для ЭВМ № 2025687778. Российская Федерация. Клиническая навигация 3D-анатомического и 3D-ультраструктурного состояния почечной паренхимы по результатам ПЭТ/КТ с 11С-холином. Заявл. 14.07.2025; опубл. 15.10.2025. EDN SXBZXD. [Berdichevsky B.A., Berdichevsky V.B., Zubik G.V. et al. Certificate of state registration of software №2025687778. Russian Federation. Clinical navigation of 3D anatomical and 3D ultra-structural state of renal parenchyma based on PET/CT with 11C-choline. Filed 14 Jul 2025; published 15 Oct 2025.].

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2026 Bionika Media