DEVELOPMENT OF A REAL-TIME PCR-BASED TEST SYSTEM TO DETECT KEMEROVO VIRUS RNA


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Abstract

Objective. To develop a real-time PCR-based test system for the detection of Kemerovo virus RNA in different types of material examined. Subjects and methods. The fragment of polymerase gene nucleotide sequence was chosen as a target. A TagMan probe was developed for detection. Results. The real-time PCR-based test system was first developed to determine Kemerovo virus RNA. Conclusion. The use of the developed test system is scheduled to determine the basic epidemiological characteristics of Kemerovo virus in the Russian Federation.

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About the authors

V. G DEDKOV

Central Research Institute of Epidemiology, Russian Inspectorate for Protection of Consumer Right and Human Welfare

Email: vgdedkov@mail.ru

M. L MARKELOV

Ya. A VOITSEKHOVSKAYA

Central Research Institute of Epidemiology, Russian Inspectorate for Protection of Consumer Right and Human Welfare

G. G KARGANOVA

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences

A. P GMYL

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences

G. P PIVANOVA

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences

L. I KOZLOVSKAYA

M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences

G. A SHIPULIN

Central Research Institute of Epidemiology, Russian Inspectorate for Protection of Consumer Right and Human Welfare

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