Human herpesvirus 6 infection in children hospitalized with the clinical manifestations of acute respiratory disease


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Abstract

Objective. To optimize the diagnosis of active human herpesvirus 6 (HHV-6) infection in children hospitalized with clinical presentations of acute respiratory disease. Subjects and methods. Examinations were made in 567 children admitted to hospital with the clinical manifestations of acute respiratory infection (ARI) and 100 clinically healthy children at the age of 1 year to 16 years. Herpesvirus infections were diagnosed using enzyme immunoassay, real-time polymerase chain reaction, rapid culture (RC), and indirect immunofluorescence test. Results. The laboratory markers of HHV-6 infection were observed in 80% of the examinees; one third (n = 136) of them were diagnosed with acute primary and reactivated infection. Acute primary forms of infection are more frequently encountered in children during the first three years of life; the course of infection with nonspecific features is more typical for children older than 3 years of age. The presence of blood virus DNA (more than 100 copies/105 cells) in combination with the release of early antigens (RC) testifies to the active form of HHV-6 infection. A management algorithm has been elaborated for children with ARI and active forms of HHV-6 infection. Conclusion. The detection of active HHV-6 infection in children hospitalized with ARI requires an integrated diagnostic approach using quantitative determination of blood HHV-6 DNA and early viral proteins (RC).

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About the authors

A. V Gorelov

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human WellBeing

Email: crie@pcr.ru
MD, Corresponding Member of the Russian Academy of Sciences; Head, Clinical Department of Infectious Diseases Moscow, Russia

A. D Muzyka

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human WellBeing

Email: nikolitch-anna@yandex.ru
Junior Researcher, Clinical Department of Infectious Diseases Moscow, Russia

E. V Melekhina

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human WellBeing

Email: e.melekhina@mail.ru
Cand. Med. Sci., Associate Professor in Pediatrics, Senior Researcher, Clinical Department of Infectious Diseases 3a, Novogireevskaya St., Moscow 111123, Russia Теlеphone: +7(495) 672-11-58

E. V Petukhova

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human WellBeing

Email: tymrf@mail.ru
Junior Researcher, Clinical Department of Infectious Diseases Moscow, Russia

O. Yu Shipulina

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human WellBeing

Email: olga.shipulina@pcr.ms
Cand. Med. Sci., Head, Division of Molecular Diagnostics, Department of Molecular Diagnostics and Epidemiology Moscow, Russia

E. A Domonova

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human WellBeing

Email: elvira.domonova@pcr.ms
Cand. Biol. Sci., Senior Researcher, Department of Molecular Diagnostics and Epidemiology Moscow, Russia

M. Yu Lysenkova

Honored Academician N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of Russia

Email: happydoctor-mk@mail.ru
Cand. Biol. Sci., Researcher, Laboratory for Epidemiology of Opportunistic Infections Moscow, Russia

O. L Chugunova

N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia

Email: ol_chugunova@mail.ru
МD, Professor Moscow, Russia

A. S Akopyan

G.N. Speransky City Children's Clinical Hospital Nine, Moscow Healthcare Department

Email: alexandr_akopyan@bk.ru
Head, Department Moscow, Russia

V. I Barykin

Khimki Central Clinical Hospital, Khimki

Email: hcgb.muz.dio@mail.ru
Head, Children’s Infectious Department Moscow, Russia

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