The Database «Nucleotide sequences of the genome of HIV-1 isolates in the Privolzhsky Federal District»

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Objective. Optimization of epidemiological surveillance of the spread of drug-resistant HIV strains in the Privolzhsky Federal District (PFD) by introducing modern technologies for collecting, storing, processing, uploading and analyzing data obtained during studies of HIV resistance to antiretroviral drugs (ARVDs).

Materials and methods. Nucleotide sequences of the HIV-1 genome were obtained by sequencing fragments of the pol (pro, rev, int) gene of samples from infected patients from the PFD. Molecular genetic, epidemiological and clinical laboratory data were combined and integrated using the functionality of pivot tables and the built-in VBA language of Microsoft Excel into a single digital format.

Results. The database (DB) «Nucleotide sequences of the genome of HIV-1 isolates in the Privolzhsky Federal District» containing information on 1201 samples of HIV-infected patients tested for drug resistance (DR) of HIV from 2016 to 2023 was created. The DB allows analyzing the prevalence of resistance mutations, HIV subtypes, DR to ARVDs, obtaining a sample of patients matched by age, gender, stage of HIV infection, drugs and treatment regimens, adherence, clinical and laboratory parameters. Data is unloaded in Excel, PNG, JPEG, PDF formats, and nucleotide sequences - in FASTA format.

Conclusion. The presented DB optimizes the molecular epidemiological monitoring of HIV DR and the investigation of HIV infection cases, including with the help of phylogenetic analysis in the implementation of epidemiological surveillance of HIV infection in the Privolzhsky Federal District.

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作者简介

Olga Peksheva

Academician Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology

Email: peolinn@mail.ru
ORCID iD: 0000-0003-1621-4962

Clinical Laboratory Diagnostics Physician, Head of the Laboratory of the Privolzhsky District Center for AIDS Prevention and Control

俄罗斯联邦, Nizhny Novgorod

Natalya Zaitseva

Academician Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology

Email: zaytseva@nniiem.ru
ORCID iD: 0000-0001-5370-4026

МD, Director

俄罗斯联邦, Nizhny Novgorod

Olga Parfenova

Academician Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology

Email: olgaparfenova1960@mail.ru

Cand. Biol. Sci., Biologist at the Privolzhsky District Center for AIDS Prevention and Control

俄罗斯联邦, Nizhny Novgorod

Svetlana Lyamshaeva

Academician Blokhina Nizhny Novgorod Research Institute of Epidemiology and Microbiology

Email: liamchaeva@yandex.ru

Clinical Laboratory Diagnostics Physician at the Privolzhsky District Center for AIDS Prevention and Control

俄罗斯联邦, Nizhny Novgorod

Vladimir Epifanov

LLС «GC «Innotech»

编辑信件的主要联系方式.
Email: v.y.epifanov@yandex.ru

Expert at the Enterprise Management Competence Center of the Division of Implementation and Consulting

俄罗斯联邦, Moscow

参考

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1. JATS XML
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2. Fig. 1. «Main summary table» of the database

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3. Fig. 2. Form for creating a «summary table» based on certain criteria

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4. Fig. 3. Illustration of the work of the database with the selected criteria, using the example of patients with ART inefficiency in the Privolzhsky Federal District in 2016–2019 and 2021–2023

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