| Title |
Fig. 3. Results of cultivation of placental tissue cells in MethoCult® medium (Stem Cell Technologies, Canada): A - colonies of erythroid precursors (CFU-E - colony forming unit erythroid), BFU-E (burst-forming unit erythroid), granulocytes and macrophages (CFU-GM-colony forming unit-granulocyte, macrophage), CFU-GEMM (colony forming unit-granulocyte, erythroid, macrophage, megakaryocyte); B - colony phenotype after 6 weeks of cultivation on feeder cultures and 2 weeks in MethoCult® medium: B1 - CFU-E colony; B2 - CFU-GEMM colony; C - erythroblast-erythrocyte colony. Hemoglobin staining with benzidine (Sigma, USA): C1 - cells from placental tissue; C2 - cells from umbilical cord blood; D-G - colonies stained for leukocyte markers, nuclei stained with DAPI (blue); D - Staining for CD11b; E, CD31 staining; F, CD69 staining; G, negative control |