Aspirantskiy Vestnik PovolzhiyaAspirantskiy Vestnik Povolzhiya2072-23542410-3764Samara State Medical University2417810.17816/2072-2354.2016.0.5-6.26-29Research ArticleCLINICAL AND LABORATORY CHARACTERISTICS OF PATIENTS WITH HBEAG-NEGATIVE CHRONIC HEPATITIS B WITH DIFFERENT LEVELS OF VIRAL LOAD IN THE SERUMZAYCEVAE Aekasokova@mail.ruSamara State Medical University15092016165-6262911032020Copyright © 2016, ZAYCEVA E.A.2016Our study demonstrates the relationship between of viral load with clinical, biochemical and immunological (IL-2, INF-α, INF-γ) tests in patients with HBeAg-negative НBV, n=132 analyzed the relationship. It was found that 75% of patients with HBeAg-negative НBV had viral load more than 20,000 IU\ml (n=99), in other cases, the amount of virus in the blood serum was below 20.000 IU\ml (n=35). These patients comprised two comparison groups, in the control group the blood of healthy donors was studied, n=50. The study revealed that patients with a higher viral load such as clinical syndromes asthenovegetative and hepatomegaly, and changes in biochemical parameters (increased levels of total bilirubin, Alt, AST, GGT) were significantly more common (p<0.001) compared to both the comparison group and the control group. Cytokine status indicators (IL-2, INF-α, INF-γ) in initial examination of patients had significant differences depending on the initial level of viral load in the serum. When the amount of virus was over 20,000 IU\ml there was observed decrease in the concentration of cytokines, in HBV DNA below 20.000 IU\ml their level was close to reference values. The low level of cytokines IL-2, INF-α, INF-γ in patients with HBeAg-negative НBV and high viral load can be explained by the consequence of the negative impact on the immune system due to long-term persistence of immunological body reaction in HBV.IL-2INF-αINF-γviral loadIL-2INF-αINF-γхронический вирусный гепатит Ввирусная нагрузкаchronic viral hepatitis В[Габдрахманов И.А. [и др.] Взаимосвязи вирусологических и морфологических показателей в фазах иммунного контроля и реактивации у больных хроническим гепатитом В // Журнал инфектологии. - Т.7. - № 4. - 2015. - С.37-41.][Жданов К.В. Вирусные гепатиты - СПб.: ИКФ «Фолиант», 2011. - 26 с.][Ивашкин В.Т. Иммунная система и повреждения печени при хронических гепатитах В и С // Рос. журн. гастроэнтерологии, гепатологии, колопроктологии. - 2009. - № 6. - С. 4-10.][Ивашкин В.Т. Механизмы иммунной толерантности и патологии печени // Рос. журн. гастроэнтерологии, гепатологии, колопроктологии. - 2009. - № 2. - С. 8-13.][Карандашова И.В., Чуланов В.П. Особенности лабораторной диагностики инфекций. Вирусные гепатиты. Гепатит В // Лабораторная диагностика инфекционных болезней. Справочник / Под ред. В.П. Покровского, М.Г. Твороговой, Г.А. Шипулина. - М.: БИНОМ, 2013. - С. 62-74.][Юнкеров В.И. Математико-статистическая обработка данных медицинских исследований / В.И. Юнкеров, С.Г. Григорьев. - 2-е изд., доп. - СПб.: ВМедА, 2005 - 292 с.][EASL clinical practice guidelines: Management of chronic hepatitis B virus infection. - J Hepatol. - 2012. - 57(1). - Р. 167-185.][Stasi C. et al. The epidemiological changes of HCV and HBV infections in the era of new antiviral therapies and the antiHBV vaccine. - Journal of infection and public health. - 2015 Jul 4. Available from: http://www.sciencedirect. com/science.][World Health Organization. Hepatitis B Fact sheet. - July 2012. Available at: http://www.who.int/mediacentre/factsheets/fs204/ en/. Accessed November 1, 2012.]