Influence of nutrient medium and air temperature on development of sclerotia of Sclerotinia sclerotiorum (Lib.) de Bary in laboratory

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Аннотация

The research was conducted to study the peculiarities of growth and development of sclerotia of the fungus S. sclerotiorum isolated from winter rapeseed and white mustard plants growing in the Western Ciscaucasia to develop the most effective measures to protect these crops from the disease. The experiments were conducted in laboratory conditions in 2024. The sclerotia of the fungus S. sclerotiorum, collected in 2023 from the affected stems of the white mustard variety Raduga (Labinsky district) and in 2024 from the affected stems of the winter rapeseed variety Loris (Krasnodar), were the object of this study. The soils are chernozems with different degrees of leaching. The laboratory study included four variants, each of which was examined using 10 S. sclerotiorum sclerotia: a nutrient semi-synthetic medium PGA (potato-glucose agar) at an air temperature of 25 °C; a nutrient semi-synthetic medium PGA at an air temperature of 27 °C; a Czapek’s nutrient synthetic medium at an air temperature of 25 °C; and a Czapek’s nutrient synthetic medium at an air temperature of 27 °C. Studies of the biological properties of S. sclerotiorum under laboratory conditions are preferably carried out using the PGA nutrient medium. The rate of pathogen sclerotia germination on PGA is higher than the rate of mycelium growth on Czapek’s agar: 1.5 times on average at 25 °C and 3.6 times on average at 27 °C. Using Czapek’s agar to cultivate S. sclerotiorum isolates slows the formation of new fungal sclerotia and reduces their number by 28–35 %.

Авторлар туралы

O. Serdyuk

Federal Scientific Center of the Pustovoit All-­Russian Research Institute of Oil Crops

Email: oserdvk@mail.ru
350038, Krasnodar, ul. Filatova, 17

Әдебиет тізімі

  1. Sclerotinia sclerotiorum (Lib.) de Bary. Index Fungorum . URL: https://www.indexfungorum.org/names/NamesRecord. asp? RecordID=212553 (дата обращения: 03.04.2025).
  2. Sclerotinia sclerotiorum (Lib.) de Bary: Insights into the Pathogenomic Features of a Global Pathogen / Md. M. Hossain, F. Sultana, W. Li, et al. // Cells. 2023. Vol. 12. No. 7. Article 1063. URL: https://www.mdpi.com/2073–4409/12/7 /1063 (дата обращения 03.04.2025) doi: 10.3390/cells12071063.
  3. Mizubuti E. S. G. Special issue on white mold – Sclerotinia sclerotiorum // Tropical Plant Pathology. 2019. Vol. 44. P. 1–2. URL: https://link.springer .com/article/10.1007/s40858-019-00276-1 (дата обращения: 03.04.2025). doi: 10.1007/s 40858-019-00276-1 .
  4. The Harmfulness of Phoma Stem Canker, Sclerotinia Stem Rot, and Phytoplasma on Winter Oilseed Rape with Regard to Czech Breeding Programs / J. Poslusna, E. Plachka, J. Horacek, et al. // Agronomy. 2019. Vol. 9. No. 2. Article 75. URL: https://www.mdpi.com/2073–4395/9/2/75 (дата обращения: 05.04.2025). doi: 10.3390/agronomy9020075.
  5. Understanding resistance mechanisms and genetic advancements for managing Sclerotinia stem rot disease in oilseed Brassica / N. C. Gupta, S. Ashraf, N. A. Bouqellah, et al. // Physiological and Molecular Plant Pathology. 2025. Vol. 136. Article 102480. URL: https://www.sciencedirect.com/science/article/ abs/pii/S0885576524002649?via%3Dihub (дата обращения: 05.04.2025). doi: 10.1016/ j.pmpp.2024.102480.
  6. Сердюк О. А., Трубина В. С., Горлова Л. А. Влияние склеротиниоза на структуру урожая горчицы белой (Sinapis alba L.) в зависимости от степени поражения растений // Защита и карантин растений. 2020. № 4. С. 44–46.
  7. Singh K. P., Kumari P., Rai P. K. Phenotypic characterization and resistance response to Sclerotinia sclerotiorum of backcross lines developed from stable allohexaploids of Sinapis alba + Brassica juncea // Euphytica. 2023. Vol. 219. Article 34. URL: https://link.springer.com/article/10.1007/s10681-023-03160-x ( дата обращения: 03.04.2025). doi: 10.1007/s10681-023-03160-x.
  8. Silicon Alleviates the Disease Severity of Sclerotinia Stem Rot in Rapeseed / Y. Feng, Y. Hu, P. Fang, et al. // Frontiers in Plant Science. 2021. Vol . 12. URL: https://www.frontiersin.org/journals/plant-science /articles/10.3389/fpls.2021.721436/full (дата обращения: 03.04.2025). doi: 10.3389/fpls.2021.721436 .
  9. Вредные организмы в посевах рапса и меры борьбы с ними / В. М. Лукомец, Н. М. Тишков, С. А. Семеренко и др. Краснодар: Просвещение-Юг, 2020. 215 с.
  10. Zawieja B., Slebioda L., Mikulski T. Progress in plant tolerance to the fungal disease Sclerotinia in breeding experiments on winter oilseed rape // Biometrical Letters. 2023. Vol. 60. No. 1. P. 23–25. doi: 10.2478/bile-2023-0002.
  11. A Global Survey on Diseases and Pests in Oilseed Rape – Current Challenges and Innovative Strategies of Control / X. Zheng, B. Koopmann, B. Ulber, et al. // Frontiers in Agronomy. 2020. Vol. 2. URL: https://www.frontiersin.org/journals/agronomy/articles/10.3389/ fagro.2020.590908/full (дата обращения: 03.04.2025). doi: 10.3389/fagro.2020.590908 .
  12. Morphological and Patogenic Characterization of Sclerotinia sclerotiorum / T. P. Zanatta, S. M. Kulczynski, C. W. Guterres, et al. // Journal of Agricultular Science. 2019. Vol. 11. No. 8. P. 302– 313. https://www.researchgate.net/publication/333803735_Morphological_and_Patogenic_Characterization_of_Sclerotinia_sclerotiorum doi: 10.5539/jas.v11n8p302 .
  13. The schizotrophic lifestyle of Sclerotinia sclerotiorum / O. Shang, D. Jiang., J. Xie, et al. // Molecular Plant Pathology. 2024. Vol . 25. No. 2. 13 p. URL: https://bsppjournals.onlinelibrary.wiley .com/doi/10.1111/mpp.13423 (дата обращения: 05.04.2025). doi: 10.1111/mpp.13423 .
  14. Abdullah M. T., Ali N. Y., Suleman P. Effect of Salinity, Temperature and Carbon Source on the Growth and Development of Sclerotia of Sclerotinia sclerotiorum Isolated from Semi-arid Environment // Journal of Plant Pathology. 2008. Vol. 24. No. 4. P . 407–416. doi: 10.5423/PPJ.2008.24.4.407.
  15. Morphological development of sclerotia by Sclerotinia sclerotiorum: a view from light and scanning electron microscopy / C. Ordonez-Valencia, R. Ferrera-Cerrato, R. E. Quintanar-Zuniga, et al. // Annals of Microbiology. 2015. No. 65. P. 765–770. doi : 10.1007/s13213-014-0916-x.
  16. Dassanayaka M. P., Casonato S. G., Jones E. E. In vitro inhibition of Sclerotinia sclerotiorum mycelial growth and reduction of sclerotial viability by the volatile bioactive compounds of Brassicaceae crops // Journal of Applied Microbiology. 2023. Vol. 134. No. 12. Article 289. URL: https://academic.oup.com/jambio/article/134/12/lxad289/7455883 (дата обращения: 03.04.2025). doi: 10.1093/jambio/lxad289.
  17. Вознесенский В. Л. Первичная обработка экспериментальных данных: Практические приемы и примеры . Л.: «Наука», 1969. С. 32 – 33.

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