In vitro regeneration features in some varieties of Phlox paniculata L., promising for use in landscape compositions

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Abstract

The article presents the results of a study of variety-specific features of Phlox paniculata L. ('Venets', 'Zolotaya Rus', 'Knyaz Serebryany', 'Lunny Kamen', 'Phobus', 'Esmeralda', 'Geniy' and 'Peppermint Twist') regeneration under in vitro conditions. The highest values of the multiplication coefficient, determined by the number of microshoots, were established for three phlox varieties: 'Geniy' — 29,2 ± 3,3; 'Zolotaya Rus' — 23,8 ± 2,7; 'Esmeralda' — 23,5 ± 4,1. Low ability to reproduce in vitro, caused mainly by a small number of microshoots, was revealed in two phlox varieties: 'Peppermint Twist' — 8,7 ± 0,5 and 'Venets' — 8,7 ± 1,6. The maximum number of microshoots was determined: in the 'Geniy' variety — 10,4 ± 0,8 pcs., the minimum — in the 'Peppermint Twist' variety — 1,2 ± 0,1 pcs. The highest number of nodes was noted in the 'Peppermint Twist' variety — 6,9 ± 0,6 pcs./explant, the smallest — in the 'Zolotaya Rus' variety — 2,9 ± 0,4 pcs./explant. A conditional division of all studied Phlox paniculata L. varieties into three groups based on in vitro propagation intensity is proposed: 1) 'Genius', 'Golden Rus', and 'Esmeralda'; 2) 'Phobus'; 3) 'Peppermint Twist', 'Venets', 'Prince Silver', and 'Lunnyy Kamen'. The presence of a root system and callus at the base of the explants was recorded for most varieties after 60 days of cultivation.

About the authors

Natalya A. Mamaeva

The N.V. Tsitsin Main Botanical Garden of the Russian Academy of Sciences

Author for correspondence.
Email: mamaeva_n@list.ru

Cand. Sci. (Biology), Senior Researcher of Plant Biotechnology Laboratory

Russian Federation, 4, Botanicheskaya St., 127276, Moscow

Olga I. Molkanova

The N.V. Tsitsin Main Botanical Garden of the Russian Academy of Sciences

Email: molkanova@mail.ru

Cand. Sci. (Agr.), Leading Researcher, Head of Plant Biotechnology Laboratory

Russian Federation, 4, Botanicheskaya St., 127276, Moscow

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Supplementary files

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2. Phlox in flower compositions

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3. Fig. 1. Microshoots of Ph. paniculata L. varieties formed from different types of renewal buds in vitro: а — lateral; б — apical; в — adventitious; г — mixed type

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4. Fig. 2. Ph. paniculata L. varieties characterized by maximum and minimum in vitro multiplication rates: a — 'Genius'; б — 'Venets'

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5. Fig. 3. Structure elements of the composite feature "the number of micro-shoots" in the studied Ph. paniculata L. cultivars

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6. Fig. 4. Structure elements of the composite feature the number of nodes in the studied Ph. paniculata L. cultivars

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Copyright (c) 2025 Mamaeva N.A., Molkanova O.I.

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