Photosynthetic activity analysis for Picea and Abies of different sanitary conditions

Cover Page

Cite item

Full Text

Abstract

Photosynthesis is the process that provides a plant cell with energy, therefore, the preservation of photosynthetic activity under conditions of physiological stress largely determines the plant’s resistance to adverse environmental factors. The improvement of methods that make it possible to monitor changes in the state of the photosynthetic apparatus is of both theoretical and practical importance. Photosynthetic processes were studied by pulse-amplitude fluorimetry using the WALZ JUNIOR-PAM instrument in coniferous trees (spruce and fir) of different health categories growing in the Perm region of Russia. The measurements carried out using the PAM fluorimeter consist of three components: measuring light, actinic light and saturation pulses. Photosynthetic activity is induced by actinic light, and saturation pulses are used to determine the maximum fluorescence output. The third component, modulated measuring light, does not cause photosynthesis by itself, but measures/observes changes in (fluorescent) output. The fluorometer registers only the fluorescence reaction caused by modulated light. The study assessed the effective quantum yield of Y(II), the electron transport density along the electron transport chain of thylakoid membranes (ETR), the non-photochemical quenching of chlorophyll fluorescence (NPQ), and the coefficient of photochemical quenching of chlorophyll fluorescence (qP). It was found that the ETR, Y(II), NPQ, and qP indices in trees reflect their individual condition. In trees of the 4th category of sanitary condition, these indices are significantly lower than in trees of higher categories, and the qP index is zero or close to zero.

About the authors

Irina L. Bukharina

Udmurt State University

Author for correspondence.
Email: buharin@udmlink.ru

Dr. Sci. (Biology), Professor, Director

Russian Federation, 1/1, Universitetskaya st., 426034, Izhevsk, Udmurtia

Maksim V. Larionov

Russian Biotechnological University (ROSBIOTEC’H University)

Email: m.larionow2014@yandex.ru

Dr. Sci. (Biology), Professor

Russian Federation, 33, Talalikhina st., 109316, Moscow

Anna S. Pashkova

Udmurt State University

Email: elena7108@yandex.ru

Cand. Sci. (Biology), Associate Professor

Russian Federation, 1/1, Universitetskaya st., 426034, Izhevsk, Udmurtia

Konstantin E. Vedernikov

Udmurt State University

Email: wke-les@rambler.ru

Cand. Sci. (Biology), Associate Professor

Russian Federation, 1/1, Universitetskaya st., 426034, Izhevsk, Udmurtia

Aleksandra S. Belelia

Udmurt State University

Email: alex.belelya@gmail.com

pg.

Russian Federation, 1/1, Universitetskaya st., 426034, Izhevsk, Udmurtia

References

  1. Maxwell K., Johnson G.N. Chlorophyll fluorescence – a practical guide. Journal of Experimental Botany, 2000, no. 51 (345), pp. 659–668.
  2. Vedernikov K.E., Bukharina I.L. Osobennosti vidovogo sostava narushennykh elovykh ekosistem [Species composition of disturbed spruce ecosystems]. Problemy populyatsionnoy biologii: materialy XIII Vserossiyskogo Populyatsionnogo seminara s mezhdunar. uchastiem pamyati N. V. Glotova (k 85-letiyu so dnya rozhdeniya) [Problems of Population Biology: Proceedings of the XIII All-Russian Population Seminar with international participation in memory of N. V. Glotov (on his 85th anniversary)]. Ufa, April 9–11, 2024. Ufa: Aeterna, 2024, pp. 91–97.
  3. Gubanova T.B., Pil’kevich R.A., Kharchenko A.A., Bernatskiy I.V. Vliyanie zasukhi na sostoyanie fotosinteticheskogo apparata nekotorykh sortov Ficus carica [Effect of drought on the state of photosynthetic apparatus of some Ficus carica cultivars]. Biologiya rasteniy i sadovodstvo: teoriya, innovatsii [Plant Biology and Horticulture: Theory, Innovations], 2019, no. 151, pp. 109–119.
  4. Platovskiy N., Zdioruk N., Ralya T. Primenenie metoda fluorimetrii dlya otsenki pervichnoy teploustoychivosti flagovykh list’ev geksaploidnoy pshenitsy v zavisimosti ot temperatury teplovogo shoka [Application of the fluorimetry method to assess the primary heat resistance of hexaploid wheat flag leaves depending on the heat shock temperature]. Buletinul AŞM. Ştiinţele vieţii. Fiziologia şi Biochimia Plantelor, 2020, no. 2(341), pp. 67–72.
  5. Kunina V.A. Ispol’zovanie khlorofill-fluorestsentsii dlya diagnostiki funktsional’nogo sostoyaniya rasteniy (literaturnyy obzor) [Use of chlorophyll fluorescence for diagnostics of the functional state of plants (literature review)]. Subtropicheskoe i dekorativnoe sadovodstvo [Subtropical and ornamental gardening], 2022, no. 83, pp. 157–166. doi: 10.31360/2225-3068-2022-83-167-180
  6. Shuliko N.N., Yusova O.A, Kiseleva A.A., Kubasova E.V., Tukmacheva E.V., Yusov V.S. Primenenie khlorofill-fluorestsentsii dlya diagnostiki funktsional’nogo sostoyaniya rasteniy yarovoy pshenitsy pri sozdanii rizosfernoy azotfiksiruyushchey assotsiatsi [Application of chlorophyll fluorescence for diagnostics of functional state of spring wheat plants during creation of rhizosphere nitrogen-fixing association]. Agrofizika [Agrophysics], 2024, no. 3, pp. 35–47.
  7. Pinyaskina E.V., Mammaev A.T. Izuchenie parametrov fotosinteticheskoy aktivnosti rasteniy v zavisimosti ot vertikal’noy poyasnosti [Study of photosynthetic activity parameters of plants depending on vertical zonality]. Izvestiya Samarskogo nauchnogo tsentra RAN [Proceedings of the Samara Scientific Center of the Russian Academy of Sciences], 2014, no. 1–3, pp. 788–791.
  8. Forzieri G., Dakos V., McDowell N.G., Alkama R., Cescatti A. Emerging signals of declining forest resilience under climate change. Nature, 2022, v. 608 (7923), pp. 534–539. doi: 10.1038/s41586-022-05071-9
  9. Constandache C., Tudor C., Aga V., Popovici L. Ecological restoration of Norway spruce stands affected by drying from outside the natural range. Scientific Papers. Series E. Land Reclamation, Earth Observation & Surveying, Envi-ronmental Engineering, 2024, v. XIII, pp. 60–66.
  10. Patacca M., Lindner M., Lucas-Borja M.E. Significant increase in natural disturbance impacts on Euro-pean forests since 1950. Global Change Biology, 2023, v. 29(5), pp. 1359–1376. DOI:0.1111/gcb.16531
  11. Vedernikov K.E., Bukharina I.L., Udalov D.N., Pashkova A.S., Larionov M.V., Mazina S.E., Galieva A.R. The State of Dark Coniferous Forests on the East European Plain Due to Climate Change. Life, 2022, v. 12, no. 11, p. 1874.
  12. Shmakova N.Yu., Markovskaya E.F., Ermolaeva O.V., Morozova K.V. Fotosinteticheskiy apparat Taraxacum arcticum i Taraxacum officinale (Asteraceae) na Zapadnom Shpitsbergene [Photosynthetic apparatus of Taraxacum arcticum and Taraxacum officinale (Asteraceae) in Western Svalbard]. Botanicheskiy zhurnal [Botanical Journal], 2021, v. 106, no. 7, pp. 676–682.
  13. Kalaji H.M., Jajoo A., Oukarroum A., Brestic M., Zivcak M., Samborska I., Cetner M.D., Goltsev V., Ladle R.J., Dąbrowski P., Ahmad P. The Use of Chlorophyll Fluorescence Kinetics Analysis to Study the Performance of Photosynthetic Machinery in Plants. Emerging Technologies and Management of Crop Stress Tolerance, 2014, v. 2, pp. 347–385.
  14. Makarenko M.S., Kozel N.V., Usatov A.V., Gorbachenko O.F., Averina N.G. A state of PSI and PSII photochemistry of sunflowеr yellow-green plastome mutant. Online J. of Biological Sciences, 2016, v. 16, no. 4, pp. 193–198.
  15. Matuszyńska A., Saadat N.P., Ebenhöh O. Balancing energy supply during photosynthesis – a theoretical perspective. Physiologia Plantarum, 2019, v. 166, no. 1, pp. 392–402.
  16. Sukhova E.M., Vodeneev V.A., Sukhov V.S. Matematicheskoe modelirovanie fotosinteza i analiz produktivnosti rasteniy [Mathematical modeling of photosynthesis and analysis of plant productivity]. Biologicheskie membrany [Biological membranes], 2021, v. 38, no. 1, pp. 20–43.
  17. Govindjee Photosystem II heterogeneity: the acceptor side. Photosynth. Res. 1990, v. 25, no. 3, pp. 151–160.
  18. Smolikova G.N., Lebedev V.N., Lopatov V.E., Timoshchuk V.A., Medvedev S.S. Dinamika fotokhimicheskoy aktivnosti fotosistemy II pri formirovanii semyan Brassica nigra L. [Dynamics of photochemical activity of photosystem II during seed formation of Brassica nigra L.]. Vestnik Sankt-Peterburgskogo Universiteta. Seriya 3: Biologiya [Bulletin of St. Petersburg University. Series 3: Biology], 2015, no. 3, pp. 53–65.
  19. Shimkevich A.M., Makarov V.N., Goloenko I.M., Davydenko O.G. Funktsional’noe sostoyanie fotosinteticheskogo apparata u alloplazmaticheskikh liniy yachmenya [Functional state of photosynthetic apparatus in alloplasmic lines of barley]. Ekologicheskaya genetika [Ecological Genetics], 2006, v. IV, no. 2, pp. 37–42.
  20. Gayevsky N.A. Morgun V.N. The use of variable and delayed chlorophyll fluorescence for studying plant photosynthesis. Plant Physiology, 2007, v. 40, pp. 136–145.
  21. Kabashnikova L.F., Domanskaya I.N., Pashkevich L.V., Dremuk I.A., Martysyuk A.V., Molchan O.V. Vliyanie intensivnosti sveta i ego spektral’nogo sostava na fotosinteticheskuyu aktivnost’ ogurtsa Cucumis sativus pri fuzarioznom zarazhenii [Effect of light intensity and its spectral composition on photosynthetic activity of cucumber Cucumis sativus under Fusarium infestation]. Eksperimental'naya biologiya i biotekhnologiya [Experimental Biology and Biotechnology], 2022, no. 3, pp. 39–52.
  22. Ovsyannikov A.Yu. Sezonnaya strukturno-funktsional’naya transformatsiya fotosinteticheskogo apparata khvoi Picea pungens Engl. i P. obovata Ledeb. na territorii botanicheskogo sada URO RAN (g. Ekaterinburg) [Seasonal structural and functional transformation of the photosynthetic apparatus of the needles of Picea pungens Engl. and P. obovata Ledeb. on the territory of the botanical garden of the URO RAS (Ekaterinburg)]. Dis. Cand. Sci. (Biol.) 03.02.08. Ekaterinburg, 2015, 148 р.
  23. Andreev V.P., Plakhotskaya Zh.V. Deystvie presnoy vody na parametry induktsii fluorestsentsii predstaviteley roda Fucus v usloviyakh Belogo morya [The effect of fresh water on the parameters of fluorescence induction of representatives of the genus Fucus in the White Sea]. Izvestiya vuzov. Prikladnaya khimiya i biotekhnologiya [Izvestiya Vuzov. Applied chemistry and biotechnology], 2017, v. 7, no. 3, pp. 75–83.
  24. Baker N.R., Rosenqvist E. Applications of chlorophyll fluorescence can improve crop production strategies: An examination of future possibilities. J. of Experimental Botany, 2004, v. 55, pp. 1607–1621.
  25. Holzwarth A.R., Lenk D., Jahns P. On the analysis of nonphotochemical chlorophyll fluorescence quenching curves I. Theoretical considerations. Biochimica et Biophyica Acta (BBA). Bioenergetics, 2013, v. 1827, pp. 786–792.
  26. Danilova E.D., Efimova M.V., Kolomeychuk L.V., Kuznetsov V.V. Melatonin podderzhivaet fotokhimicheskuyu aktivnost’ assimilyatsionnogo apparata i zamedlyaet starenie list’ev odnodol’nykh rasteniy [Melatonin supports photochemical activity of the assimilatory apparatus and slows down aging of leaves of monocotyledonous plants]. Doklady Rossiyskoy akademii nauk. Nauki o zhizni [Reports of the Russian Academy of Sciences. Life Sciences], 2020, v. 495, no. 1, pp. 545–550.
  27. Pakhar’kova N.V., Masentsova I.V., Gette I.G., Pozdnyakova E.E., Kalabina A.A. Fotosinteticheskiy apparat khvoi sosny sibirskoy kedrovoy v period vykhoda iz sostoyaniya zimnego pokoya v usloviyakh vysotnoy poyasnosti Zapadnogo Sayana [Photosynthetic apparatus of Siberian pine needles during the period of emergence from the state of winter dormancy in the conditions of altitudinal belt of the Western Sayan]. Russian Forest J., 2024, no. 2, pp. 41–49.
  28. Kalaji H.M., Schansker G., Ladle R.J., Goltsev V., Bosa K., Allakhverdiev S.I., Brestic M., Bussotti F., Calatayud A., Dąbrowski P., Elsheery N.I., Ferroni L., Guidi L., Hogewoning S.W., Jajoo A., Misra A.N., Nebauer S.G., Pancaldi S., Penella C., DorothyBelle P., Pollastrini M., Romanowska-Duda Z.B., Rutkowska B., Serodio J., Suresh K., Szulc W., Tambussi E., Yanniccari M., Zivcak M. Frequently asked questions about in vivo chlorophyll fluorescence: practical issues. Photosynth Research, 2014, v. 122, pp. 121–158.
  29. Lichtenthaler H.K., Buschmann C. and Knapp M. How to Correctly Determine the Different Chlorophyll Fluorescence Parameters and the Chlorophyll Fluorescence Decrease Ratio RFd of Leaves with the PAM Fluorometer. Photosynthetica. Photosynthetica, 2005, no. 43 (3), pp. 379–393.
  30. Hua W., Zhu J., Shang Y., Wang J., Jia Q., Yang J. Identification of suitable reference genes for barley gene expression under abiotic stresses and hormonal treatments. Plant Molecular Biology Reporter, 2015, v. 33, pp. 1002–1012.
  31. Prikaz Ministerstva prirodnykh resursov i ekologii Rossiyskoy Federatsii ot 18 avgusta 2014 g. № 367 «Ob utverzhdenii Perechnya lesorastitel’nykh zon Rossiyskoy Federatsii i Perechnya lesnykh rayonov Rossiyskoy Federatsii» [Order of the Ministry of Natural Resources and Environment of the Russian Federation dated August 18, 2014 No. 367 «On approval of the List of forest vegetation zones of the Russian Federation and the List of forest regions of the Russian Federation». Available at: https://docs.cntd.ru/document/420224339 (accessed 20.11.2024).
  32. Postanovlenie Pravitel'stva Rossiyskoy Federatsii ot 20 maya 2017 g. № 607 «Ob utverzhdenii Pravil sanitarnoy bezopasnosti v lesakh» [Resolution of the Government of the Russian Federation of May 20, 2017, no. 607 «On Approval of the Rules of Sanitary Safety in Forests»]. Available at: http://docs.cntd.ru/document/436736467 (accessed 20.11.2024).
  33. Postanovlenie Pravitel’stva Rossiyskoy Federatsii ot 28 iyulya 2020 g. № 132 «O priznanii utrativshimi silu nekotorykh aktov i otdel’nykh polozheniy nekotorykh aktov Pravitel’stva Rossiyskoy Federatsii i ob otmene nekotorykh aktov federal’nykh organov ispolnitel’noy vlasti, soderzhashchikh obyazatel’nye trebovaniya, soblyudenie kotorykh otsenivaetsya pri provedenii meropriyatiy po kontrolyu pri osushchestvlenii federal’nogo gosudarstvennogo lesnogo nadzora (lesnoy okhrany), federal’nogo gosudarstvennogo pozharnogo nadzora v lesakh i gosudarstvennogo nadzora v oblasti semenovodstva v otnoshenii semyan lesnykh rasteniy» [Resolution of the Government of the Russian Federation of July 28, 2020, no. 132 «On the invalidation of some acts and certain provisions of some acts of the Government of the Russian Federation and on the abolition of some acts of federal executive authorities containing mandatory requirements, compliance with which is assessed during control measures in the implementation of Federal State Forest Supervision (Forest Protection), federal state fire supervision in forests and state supervision in the field of seed production»]. Available at: https://docs.cntd.ru/document/565438868?marker=65A0IQ (accessed 14.11.2024).
  34. Leskinen P., Lindner M., Verkerk P.Y., Nabuurs G.Ya., Van Brusselen Y., Kulikova E., Khassegava M., Lerink B. Lesa Rossii i izmenenie klimata [Forests of Russia and Climate Change]. Chto nam mozhet skazat’ nauka [What Science Can Tell Us], 2020, 142 p. DOI: https://doi.org/10.36333/wsctu11
  35. Thorn S., Seibold S., Leverkus A.B., Michler T., Müller J., Noss R.F., Stork N., Vogel S., Lindenmayer D.B. The living dead: acknowledging life after tree death to stop forest degradation. Frontiers in Ecology and the Environment, 2020, v. 18 (9), pp. 505–512. doi: 10.1002/fee.2252
  36. Ivanchina L.A., Bol'shakov E.G. Vliyanie usykhaniya na izrezhivanie elovykh drevostoev i na izmenenie porodnogo sostava lesnykh nasazhdeniy Permskogo kraya [he Impact of Drying Out on Thinning of Spruce Stands and Changes in Species Composition of Forest Plantations in Perm Krai]. Lesotekhnicheskiy zhurnal [Forest Engineering Journal], 2024, v. 14, no. 4 (56), pp. 6–21. DOI: https://doi.org/10.34220/issn.2222-7962/2024.4/1
  37. Burton P.J., Jentsch A., Walker L.R. The Ecology of Disturbance Interactions. BioScience, 2020, v. 70, no. 10, pp. 854–870. doi: 10.1093/biosci/biaa088
  38. Leverkus A.B., Buma B., Wagenbrenner J., Burton P.J., Lingua E., Marzano R., Thorn S. Tamm review: Does salvage logging mitigate subsequent forest disturbances? Forest Ecology and Management, 2021, v. 481, p. 118721. https://doi.org/10.1016/j.foreco.2020.118721
  39. Terinov N.N., Andreeva E.M., Zalesov S.V., Luganskiy N.A., Magasumova A.G. Vosstanovlenie elovykh lesov: teoriya, otechestvennyy opyt i metody resheniya [Restoration of spruce forests: theory, domestic experience and solution methods]. Russian Forestry J., 2020, no. 3, pp. 9–23. doi: 10.37482/0536-1036-2020-3-9-23
  40. Andreev V.P., Maslov Yu.I., Sorokoletova E.F. Functional properties of photosynthetic apparatus in three Fucus species inhabiting the White sea: effect of dehydration. Fiziologiya rastenii. Russian J. of Plant Physiology, 2012, v. 59, no. 2, pp. 244–250.
  41. Junior-PAM Chlorophyll Fluorometer: Operator’s Guide. Ed. by E. Pfùndel. Germany, Heinz Walz GmbH, 2007, 58 p.
  42. Gol’tsev V. N., Kaladzhi Kh. M., Paunov M., Baba V., Khorachek T., Moyskiy Ya., Kotsel Kh., Suleyman I A. Ispol’zovanie peremennoy fluorestsentsii khlorofilla dlya otsenki fiziologicheskogo sostoyaniya fotosinteticheskogo apparata rasteniy [Using variable chlorophyll fluorescence to assess the physiological state of the photosynthetic apparatus of plants]. Fiziologiya rasteniy [Plant Physiology], 2016, v. 63, no. 6, pp. 881–907.
  43. Domanskiy V.P. Distantsionnoe izmerenie parametrov peremennoy fluorestsentsii rastitel’nykh ob’ektov [Remote measurement of variable fluorescence parameters of plant objects]. Zhurnal prikladnoy spektroskopii [J. of Applied Spectroscopy], 2018, v. 85, no. 3, pp. 480–485.
  44. Zul’fugarov I.S., Pashaeva A., Okhlopkova Zh.M., Chun-Khvan Li. Prakticheskoe rukovodstvo po izmereniyu urovnya fluorestsentsii khlorofilla v rasteniyakh i raschetu osnovnykh parametrov fluorestsentsii khlorofilla [Practical guide for measuring the level of chlorophyll fluorescence in plants and calculation of the main parameters of chlorophyll fluorescence]. Vestnik SVFU, 2018, no. 2 (64), pp. 35–44.
  45. Zaks J., Amarnath K., Kramer D.M., Niyogi K.K., Fleming G.R. A kinetic model of rapidly reversible nonphotochemical quenching. Proceedings of the National Academy of Sciences of the USA, 2012, v. 109, no. 39, pp. 15757–15762.
  46. Makarenko M.S., Kozel N.V., Usatov A.V., Gorbachenko O.F., Averina N.G. A State of PSI and PSII Photochemistry of Sunflower Yellow-Green Plastome Mutant. OnLine J. of Biological Sciences, 2016, no. 16 (4), pp. 193–198.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2025 Bukharina I.L., Larionov M.V., Pashkova A.S., Vedernikov K.E., Belelia A.S.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 68118 от  21.12.2016.