Molecular genetic analysis of Quercus robur L populations based on polymorphism of ISSR- and SSR-markers

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Abstract

A molecular genetic analysis of two populations of Quercus robur L from the Volgograd region was carried out using ISSR and SSR analysis methods (the first population from the arboretum plantations of the Federal Research Center for Agroecology of the Russian Academy of Sciences, Volgograd, the second population from the Nizhnevolzhsky Experimental Station for tree breeding; Kamyshin). Nine of the 18 ISSR primers tested were found to be most effective for analyzing polymorphism within and between populations. The number of loci in the spectrum ranged from 4 to 10, depending on the primer. The UBC835 primer produced the largest number of loci in the spectrum (n = 10), and the greatest polymorphism (50…57 %) was found using UBC815, UBC834, UBC835, UBC836, and UBC840 primers. The main parameters of the genetic diversity of populations have been determined. It was found that all indicators were slightly higher in the first population (na = 1,75 ± 0,44, ne = 1,44 ± 0,38, HE = 0,25 ± 0,19). In the first population, polymorphism was higher by 10 %, meaning that the second population contains genetically more homogeneous trees. The electrophoregram obtained with UBC811 and UBC834 primers revealed unique DNA fragments characterizing variability within the species, which can be used in further breeding work. It has been shown that the use of SSR primers is less effective in assessing the polymorphism of these populations of Quercus robur L in comparison with ISSR primers. However, the microsatellite primers studied are species-specific, which makes it possible to use them, for example, in the genetic certification of a species.

About the authors

Anna F. Ryabukha

Federal Scientific Center for Agroecology, Integrated Land Reclamation and Protective Afforestation of the Russian Academy of Sciences

Author for correspondence.
Email: ryabuha-af@vfanc.ru

Cand. Sci. (Pharmaceutical), Leading Researcher at the Laboratory of Molecular breeding

Russian Federation, 97, Universitetskiy av., 400062, Volgograd

Petr A. Kuz’min

Federal Scientific Center for Agroecology, Integrated Land Reclamation and Protective Afforestation of the Russian Academy of Sciences

Email: kuzmin-p@vfanc.ru

Cand. Sci. (Agriculture), Leading Researcher, Acting Head of Laboratory of Molecular breeding

Russian Federation, 97, Universitetskiy av., 400062, Volgograd

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