Lignosulfonates bioprocessing

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Abstract

The lignin liquors recycling method to obtain yeast biomass by using the producer of baker's yeast Saccharomyces cerevisiae in the course of biosynthesis on a nutrient medium including waste from the pulp and paper industry such as lignosulfonates is described. It is shown that lignosulfonate, as a product of processing wood biomass, can be utilized in the process of bioprocessing in the presence of yeast into nutritious biomass, which is a protein ingredient for subsequent balancing, for example, of animal feed. Analysis of the carbohydrate composition of free sugars in the lignosulfonate part of the initial nutrient medium showed that the ratio of the main carbohydrates: arabinose, galactose, glucose, mannose, xylose, ribose and lactose was 1 : 1,6 : 1,8 : 6,4 : 12,5 : 0,1 : 0,02, which is likely to be used as a nutrient component in biotechnology. The methodology of biosynthesis was determined, optimal conditions for the process using lignosulfonate culture fluid were found, including (g/l of tap water): ammonium hydrogen phosphate — 1; potassium dihydrogen phosphate — 2; potassium hydrogen phosphate — 0,1; potassium chloride — 1,5; magnesium sulfate — 0,5; ammonium sulfate — 3; lignosulfonate — 2,5. It was shown that the process of yeast cultivation at a temperature of 35…40°C and pH 5,0 allowed obtaining a suspension containing 12…15 g/l of raw yeast cells in 8…10 hours, which can then be separated and used. The study of the amino acid and fatty-acid composition of the biomass confirmed the high biological value of the product, which may be of interest for use in cattle breeding.

About the authors

Andrey N. Ivankin

BMSTU (Mytishchi branch)

Author for correspondence.
Email: aivankin@bmstu.ru

Dr. Sci. (Chem.), Member of The International Higher Education Academy Of Sciences (IHEAS), Professor of the Department of Chemistry

Russian Federation, 1, 1st Institutskaya st., 141005, Mytishchi, Moscow reg.

Angella N. Zarubina

BMSTU (Mytishchi branch)

Email: zarubina@bmstu.ru

Cand. Sci. (Tehn.), Associate Professor, Head of the Department of Chemistry and Chemical Technologies of the Forest Complex

Russian Federation, 1, 1st Institutskaya st., 141005, Mytishchi, Moscow reg.

References

  1. Wang Y., Lyu B., Fu H., Li J., Ji L., Gong H., Zhang R., Liu J., Yu H. The development process of plant-based meat alternatives: Raw material formulations and processing strategies. Food Research International, 2023, v. 167, no. 5, p. 112689. https://doi.org/10.1016/j.foodres.2023.112689
  2. Zhang J., Meng Z., Cheng Q., Li Q., Zhang Y., Li L., Shi A., Wang Q. Plant-based meat substitutes by high-moisture extrusion: Visualizing the whole process in data systematically from raw material to the products. J. of Integrative Agriculture, 2022, v. 21, no. 8, pp. 2435–2444. https://doi.org/10.1016/S2095-3119(21)63892-3
  3. Leontovich V.P. Rastitel’nye otkhody i perspektiva ikh ispol’zovaniya [Plant waste and prospects for their use]. Kormoproizvodstvo [Feed Production], 2010, no. 1, pp. 44–46.
  4. Wang Q., Zhang Y., Ma K. Study of the differences in collection scope of raw materials of biomass CHP plants caused by regional factors. J. of Environmental Management, 2024, v. 360, no. 6, p. 121106. https://doi.org/10.1016/j.jenvman.2024.121106
  5. Zhang K., Zhang W., Xie W., Luo Y., Wei G. Investigation of mechanical properties, chemical composition and microstructure for composite cementitious materials containing waste powder recycled from asphalt mixing plants. J. of Building Engineering, 2024, v. 96, no. 11, p. 110362. https://doi.org/10.1016/j.jobe.2024.110362
  6. Kuznetsova T.G., Ivankin A.N., Kulikovsky A.V. Nanosensornyy analiz myasnogo syr’ya i rastitel’nykh ob’ektov [Nanosensory analysis of meat raw materials and plant objects]. Saarbrücken: LAP LAMBERT, 2012, 232 p.
  7. Bouzid H.A., Ibourki M., Hamdouch F., Oubannin S., Asbbane A., Hallouch O., Bijla L., Koubachi J., Majourhat K., Gharby S. Moroccan aromatic and medicinal plants: A review of economy, ethnobotany, chemical composition, and biological activities of commonly used plants. Food and Humanity, 2024, v. 2, no. 5. https://doi.org/10.1016/j.foohum.2024.100259
  8. Vaglica А., Porrello F., Ilardi V., Bruno M. The essential oil chemical composition of a rare ethnopharmacoligical plant. Natural Product Research. 2024, no. 7. https://doi.org/10.1080/14786419.2024.2377310
  9. Ivankin A.N., Chernukha I.M., Kuznetsova T.G. O kachestve rastitel’nykh i zhivotnykh zhirov [On the quality of vegetable and animal fats]. Maslozhirovaya promyshlennost’ [Oil and Fat Industry], 2007, no. 2, pp. 8–11.
  10. Puss K.K., Paaver P., Loog M., Salmar S. Ultrasound effect on a biorefinery lignin-cellulose mixture. Ultrasonics Sonochemistry, 2024, v. 111, no. 12, p. 107071. https://doi.org/10.1016/j.ultsonch.2024.107071
  11. Lan H.N., Liu R.Y., Liu Z.H., Li X., Li B.Z., Yuan Y.J. Biological valorization of lignin to flavonoids. Biotechnology Advances, 2023, v. 64, no. 5, p. 108107. https://doi.org/10.1016/j.biotechadv.2023.108107
  12. Pham C.D., Dang M.D.T., Ly T.B., Tran K.D., Vo N.T., Do N.H.N, Mai P.T., Le P.K. A review of the extraction methods and advanced applications of lignin-silica hybrids derived from natural sources. International J. of Biological Macromolecules, 2023, v. 230, no. 3, p. 123175. https://doi.org/10.1016/j.ijbiomac.2023.123175
  13. Teo H.L., Wahab R.A., Zainal-Abidin M.H., Mark-Lee W.F., Susanti E. Co-production of cellulose and lignin by Taguchi-optimized one-pot deep eutectic solvent-assisted ball milling pretreatment of raw oil palm leaves. International J. of Biological Macromolecules, 2024, v. 280, no. 11, p. 135787. https://doi.org/10.1016/j.ijbiomac.2024.135787
  14. Hou Q., Liu Z., Shi Z., Yang H., Wang D., Yang J. A deep eutectic solvent pretreatment with self-cleaning lignin droplets function to efficiently improve the enzymatic saccharification and ethanol production of bamboo residues. Industrial Crops and Products, 2024, v. 216, no. 9, p. 118730. https://doi.org/10.1016/j.indcrop.2024.118730
  15. Brienza F., Cannella D., Montesdeoca D., Cybulska I., Debecker D.P. A guide to lignin valorization in biorefineries: traditional, recent, and forthcoming approaches to convert raw lignocellulose into valuable materials and chemicals. RSC Sustainability, 2024, v. 2, no. 1, pp. 37–90. https://doi.org/10.1039/d3su00140g
  16. Frias M., Reynoso S., Rambhia S., Noki G., Olson J., Stoeber B., Trajano H.L. Effect of incubation conditions of cellulase hydrolysis on mechanical pulp fibre morphology. Carbohydrate Polymers, 2024, v. 344, no. 10, p. 122529. https://doi.org/10.1016/j.carbpol.2024.122529
  17. Jia W., Zhou M., Yang C., Zhang H., Niu M., Shi H. Evaluating process of auto-hydrolysis prior to kraft pulping on production of chemical pulp for end used paper-grade products. J. of Bioresources and Bioproducts, 2022, v. 7, no. 8, pp. 180–189. https://doi.org/10.1016/j.jobab.2022.05.002
  18. Liu H., Xu S., Li H., HeY. Exploring the dual effect of sodium lignosulfonate-modified LDH treatment and heat processing on elevating corrosion and wear resistance of Ni-W composite coatings. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2024, v. 702, part 2, no. 12, p. 135076. https://doi.org/10.1016/j.colsurfa.2024.135076
  19. Demuner I.F., Gomes B.J.B., Gomes J.S., Coura M.R., Borges F.P., Carvalho A.M., Silva C.M. Improving kraft pulp mill sustainability by lignosulfonates production from processes residues. J. of Cleaner Production, 2021, v. 317, no. 10, p. 128286. https://doi.org/10.1016/j.jclepro.2021.128286
  20. Song Y., Zhong S., Li Y., Dong K., Luo Y., Chu G., Zou H., Sun B. Study on the catalytic degradation of sodium lignosulfonate to aromatic aldehydes over nano-CuO: Process optimization and reaction kinetics. Chinese J. of Chemical Engineering, 2023, v. 53, no. 1, pp. 300–309. doi.org/10.1016/j.cjche.2021.12.028
  21. Wang J., Jiao H., Gao S., Wei J., Yu F., Xie C., Yuan B., Yu S. Potential high-energy-density biofuels from α-pinene and lignin-based phenols via alkylation and subsequent hydrodeoxygenation. Fuel, 2024, v. 374, no. 10, P. 132513. https://doi.org/10.1016/j.fuel.2024.132513
  22. Liang G., Zhang S., Xian Y., Chen L. Depressing molybdenite using calcium lignosulfonate in Cu-Mo flotation separation: Interaction and desorption insights. Advanced Powder Technology, 2024, v. 35, no. 11, p. 104665. https://doi.org/10.1016/j.apt.2024.104665
  23. Stanisz M., Smułek W., Popielski K., Klapiszewski L., Kaczorek E., Jesionowski T. Sustainable design of lignin-based spherical particles with the use of green surfactants and its application as sorbents in wastewater treatment. Chemical Engineering Research and Design, 2021, v. 172, no. 8, pp. 34–42. https://doi.org/10.1016/j.cherd.2021.05.028
  24. Xu Y., Ding H., Luo C., Zheng Y., Xu Y., Li X., Zhang Z., Shen S., Zhang L. Effect of lignin, cellulose and hemicellulose on calcium looping behavior of CaO-based sorbents derived from extrusion-spherization method. Chemical Engineering J., 2018, v. 334, no. 2, pp. 2520–2529. https://doi.org/10.1016/j.cej.2017.11.160
  25. Patel R., Babaei-Ghazvini A., Dunlop M.J., Acharya B. Biomaterials-based concrete composites: A review on biochar, cellulose and lignin. Carbon Capture Science & Technology, 2024, v. 12, no. 9, p. 100232. https://doi.org/10.1016/j.ccst.2024.100232
  26. Yang S., Li Y., Yang Y., Liu R., Zhao Y. Behavior of calcium lignosulfonate under oxygen pressure acid leaching condition. Hydrometallurgy, 2024, v. 227, no. 8, p. 106317. https://doi.org/10.1016/j.hydromet.2024.106317
  27. Feizi Z.H., Kazzaz A.E., Kong F., Fatehi P. Evolving a flocculation process for isolating lignosulfonate from solution. Separation and Purification Technology, 2019, v. 222, no. 9, pp. 254–263. https://doi.org/10.1016/j.seppur.2019.04.042
  28. Cave G., Fatehi P. Separation of lignosulfonate from spent liquor of neutral sulphite semichemical pulping process via surfactant treatment. Separation and Purification Technology, 2015, v. 151, no. 9, pp. 39–46. https://doi.org/10.1016/j.seppur.2015.07.017
  29. Yang J., Xing S., Yang W., Zhang A., Wang W. Application potential of modified waste-lignin as microbial immobilization carriers for improve soil fertility. Reactive and Functional Polymers, 2024, v. 196, no. 3, p. 105837. https://doi.org/10.1016/j.reactfunctpolym.2024.105837
  30. Kononov G.N. Dendrokhimiya. Khimiya, nanokhimiya i biogeokhimiya komponentov kletok, tkaney i organov drevesnykh rasteniy [Dendrochemistry. Chemistry, nanochemistry and biogeochemistry of components of cells, tissues and organs of woody plants]. In 2 vol. Moscow: MSFU, 2015, 1112 p.
  31. Kononov G.N., Verevkin A.N., Serdyukova Ju.V., Mironov D.A. Drevesina kak khimicheskoe syr’e. Istoriya i sovremennost’. IV. Delignifikatsiya drevesiny kak put’ polucheniya tsellyulozy. Chast’ I [Wood as chemical raw material. History and modernity. IV. Wood delignification to produce cellulose. Part I]. Lesnoy vestnik / Forestry Bulletin, 2022, vol. 26, no. 1, pp. 97–113. doi: 10.18698/2542-1468-2022-1-97-113
  32. Kiviniemi Е., Mikkola A., Mattila H., Wahlsten M., Lundell T. Oxidative stress and culture atmosphere effects on bioactive compounds and laccase activity in the white rot fungus Phlebia radiata on birch wood substrate. Current Research in Microbial Sciences, 2024, v. 7, p. 100280. https://doi.org/10.1016/j.crmicr.2024.100280
  33. Grellet M.A., Dantur K.I., Perera M.F., Ahmed P.M., Castagnaro A., Arroyo-Lopez F.N., Gallego J.B., Welin B., Ruiz M.R. Genotypic and phenotypic characterization of industrial autochthonous Saccharomyces cerevisiae for the selection of well-adapted bioethanol-producing strains. Fungal Biology, 2022, v. 126, no. 10, pp. 658–673. https://doi.org/10.1016/j.funbio.2022.08.004
  34. Ivankin A.N., Oliferenko G.L., Kulikovsky A.V. Analiticheskaya himiya [Analytical chemistry]. Moscow: Knorus, 2021, 300 p.
  35. GOST R 51880–2002. Opredelenie massovykh doley svobodnykh i obshchikh uglevodov. Metod vysokoeffektivnoy anionnoy khromatografii [Determination of mass fractions of free and total carbohydrates. High performance anion chromatography method] Moscow: Standards, 2002, 12 p.
  36. LisitsynA.B., IvankinA.N., NeklyudovA.D. Metody prakticheskoy biotekhnologii [Methods of practical biotechnology]. Moscow: VNIIMP, 2002, 408 p.
  37. Huang C., Jeuck B., Du J., Yong Q., Chang H., Jameel H., Phillips R. Novel process for the coproduction of xylooligosaccharides, fermentable sugars, and lignosulfonates from hardwood. Bioresource Technology, 2016, v. 219, no. 11, pp. 600–607. https://doi.org/10.1016/j.biortech.2016.08.051
  38. Wang Z., Li X., Liu H., Zhou T., Li J., Siddiqui M.A. Enhanced short-chain fatty acids production from anaerobic fermentation of secondary sludge by lignosulfonate addition: Towards circular economy. J. of Cleaner Production, 2024, v. 434, no. 1, p. 140252. https://doi.org/10.1016/j.jclepro.2023.140252
  39. Meledina T.V., Davydenko S.G. Drozhzhi Saccharomyces cerevisiae. Morfologiya, khimicheskiy sostav, metabolizm. [Yeast Saccharomyces cerevisiae. Morphology, chemical composition, metabolism]. St. Petersburg: ITMO University, 2015, 88 p.
  40. Kosolapov V.M., Chuikov V.A., Khudyakova H.K., Kosolapova V.G. Mineral’nye elementy v kormakh i metody ikh analiza [Mineral elements in feed and methods of their analysis]. Moscow: Ugreshskaya Printing House, 2019, 272 p.
  41. Morales-Palomo S., Tomás-Pejó E., González-Fernández C. Phosphate chelation over calcium impacts yeast growth and lipid production from short-chain fatty acids-rich media. Environmental Technology & Innovation, 2024, v. 36, no. 11, p. 103767. https://doi.org/10.1016/j.eti.2024.103767
  42. Huang Н., Xu J., Sheng Z., Xie R., Zhang H., Chen N., Li S. Effects of dietary phospholipids on growth performance, fatty acid composition, and expression of lipid metabolism related genes of juvenile hybrid. Aquaculture Reports, 2022, v. 22, no. 2, p. 100993. https://doi.org/10.1016/j.aqrep.2021.100993
  43. Veerasamy V., Neethirajan V., Singarayar M.S., Balasundaram D., Dharmar P., Thilagar S. Microalgal biomass and lipid synergy for omega fatty acid enrichment: A sustainable source for food supplements & nutraceuticals. Algal Research, v. 80, no. 6, p. 103514. https://doi.org/10.1016/j.algal.2024.103514
  44. Di Caprio F. Cultivation processes to select microorganisms with high accumulation ability. Biotechnology Advances, 2021, v. 49, no. 7, p. 107740. doi.org/10.1016/j.biotechadv.2021.107740
  45. Deive F.J., Sanroman M.A. Bioreactor development for the cultivation of extremophilic microorganisms. Current Developments in Biotechnology and Bioengineering. Bioprocesses, Bioreactors and Controls. Eds. Larroche C. et al. Elsevier, 2017, 816 p. doi.org/10.1016/B978-0-444-63663-8.00014-8
  46. Xu J., Zhou J., Du B, Li X., Huang Y., Cao Q., Xu S., Wang W. Research progress on the preparation and application of lignin-based Pickering emulsions: A review. Industrial Crops and Products, 2024, v. 222, no. 12, p. 119723. doi.org/10.1016/j.indcrop.2024.119723
  47. Ivankin A.N., Oliferenko G.L. Corrosion inhibition with green polymer systems and natural compounds. Polymer Science, Series D, 2024, v. 17, no. 4, pp. 987–994. doi: 10.1134/S1995421224701697
  48. Oliferenko G.L., Ivankin A.N., Ustyugov A.V. Kislotnaya degradatsiya drevesnykh otkhodov kak sposob polucheniya tselevykh produktov [Wood waste acid degradation to obtain target products]. Lesnoy vestnik / Forestry Bulletin, 2024, vol. 28, no. 4, pp. 130–137. doi: 10.18698/2542-1468-2024-4-130-137
  49. Wang J., Seidi F., Shi X., Li C., Huang Y., Xiao H. Unveiling the potential of dual-extrinsic/intrinsic self-healing ligninbased coatings for anticorrosion applications. International J. of Biological Macromolecules, 2025, v. 285, no. 1, p. 138073. doi.org/10.1016/j.ijbiomac.2024.138073
  50. Yin Y., Qin S., Deng S., Li Z., Tang A., Li Q., Liao D., Liu Y. Thermoresponsive lignin-based polyelectrolyte complexes for the preparation of spherical nanoparticles: Application in pesticide encapsulation. International J. of Biological Macromolecules, 2025, v. 288, no. 2, p. 138623. doi.org/10.1016/j.ijbiomac.2024.138623

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