Clonal micropropagation optimization technique for genera Heuchera L. and ×Heucherella H.R.Wehrh. promising for landscaping

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Abstract

The results of a study on improving the technique of clonal micropropagation of cultivars Heuchera L. (‘Autumn Leaves’, ‘Cherry Cola’, ‘Dew Drops’, ‘Marmalade’) and ×Heucherella H.R.Wehrh (‘Art Deco’, ‘Golden Zebra’, ‘Solar Eclipse’, ‘Plum Cascade’) are presented. A brief analysis of the qualitative and quantitative composition of the in vitro bank of representatives of the genera Heuchera, ×Heucherella and Tiaerella L. in the Laboratory of plant biotechnology of the MBG RAS is given. It was found that the influence of the concentration of 6-benzylaminopurine in the composition of the Murashige and Skoog nutrient medium on the quantitative characteristics of microrosettes in the structure of their general variability is dominant. With an increase in the concentration of 6-benzylaminopurine, a statistically significant increase in the multiplication rate was revealed in all cultivars studied in the experiment (with the exception of the cultivar ‘Dew Drops’). The formation of a small number of large microrosettes on the Murashige and Skoog nutrient medium with the absence of growth regulators was noted. The highest frequency of spontaneous rhizogenesis on the specified nutrient medium was recorded in all studied cultivars. It has been shown that meta-Topoline is preferred (relative to thidiazuron) as a growth regulator, an alternative to 6-benzylaminopurine, affecting the quantitative and qualitative characteristics of microrosettes of model cultivars Heuchera and ×Heucherella. The largest number of viable and well-developed microrosettes with a size of less than 1.0 cm, suitable for use at the multiplication stage, on a medium with the addition of thidiazuron, was recorded.

About the authors

Natal'ya A. Mamaeva

Tsitsin Main Botanical Garden the Russian Academy of Sciences

Author for correspondence.
Email: mamaeva_n@list.ru

Cand. Sci. (Biology), Senior researcher of Plant Biotechnology Laboratory

Russian Federation, 4, Botanicheskaya st., 127276, Moscow

Irina L. Krakhmaleva

Tsitsin Main Botanical Garden the Russian Academy of Sciences

Email: seglory@bk.ru

Junior researcher of Plant Biotechnology Laboratory

Russian Federation, 4, Botanicheskaya st., 127276, Moscow

Ol'ga I. Molkanova

Tsitsin Main Botanical Garden the Russian Academy of Sciences

Email: molkanova@mail.ru

Cand. Sci. (Agriculture), Leading researcher, Head of Plant Biotechnology Laboratory

Russian Federation, 4, Botanicheskaya st., 127276, Moscow

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