Regeneration and rooting features of Ribes uva-crispa L. cultivars in vitro

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The article focuses on optimizing medium composition for promising gooseberry (Ribes uva-crispa L.) cultivars at the multiplication and rooting stages. The research was carried out in the Laboratory of Plant Biotechnology of Tsitsin Main Botanical Garden of Russian Academy of Sciences in 2022. Cultivars ‘Berill’, ‘Grushen’ka’, ‘Kseniya’ and ‘Chernoslivovy’ were used in the experiments. The effect of iron chelate forms (Fe(III)-EDDHA and Fe(III)-EDTA) on morphogenetic parameters and development of gooseberry microshoots was determined. At the micropropagation stage addition of 100 mg/L Fe(III)-EDDHA to Quorin and Lepoivre medium increased the height of microshoots of the studied cultivars. The number of microshoots available for further rooting (10 mm height and above) ranged from 20 to 37 % on the media containing 100,0 mg/L Fe(III)-EDDHA compared to the media with 36,7 mg/L Fe(III)-EDTA (10...16 %). The highest morphogenetic potential was found in ‘Chernoslivovy’, which had 1,4…2,1 times higher multiplication rate than other cultivars. Rooting ability and morphometric parameters of root system were found influenced by genetic characteristics of gooseberry cultivars, type and concentration of auxins in half-strength Murashige and Skoog medium. Using of the media with 0,5...1,5 mg/L of Indolyl-3-butyric acid encouraged the formation of higher number of roots, whereas the media with 0,5...1,5 mg/L of Indolyl-3-acetic acid increased their length. Usage of medium with 0,5 mg/L Indole-3-butyric acid was found to be the most effective for rooting of most studied cultivars.

About the authors

Irina L. Krakhmaleva

Tsitsin Main Botanical Garden of the Russian Academy of Sciences

Author for correspondence.
Email: seglory@bk.ru

Junior researcher of Plant Biotechnology Laboratory

Russian Federation, 4, Botanicheskaya st., 127276, Moscow

Ol’ga V. Koroleva

Tsitsin Main Botanical Garden of the Russian Academy of Sciences

Email: elaem@yandex.ru

Researcher of Plant Biotechnology Laboratory

Russian Federation, 4, Botanicheskaya st., 127276, Moscow

Ol’ga I. Molkanova

Tsitsin Main Botanical Garden of the Russian Academy of Sciences

Email: molkanova@mail.ru

Cand. Sc. (Agr.), Leading researcher, Head of Plant Biotechnology Laboratory

Russian Federation, 4, Botanicheskaya st., 127276, Moscow

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