Lesnoy Vestnik / Forestry BulletinLesnoy Vestnik / Forestry BulletinЛесной вестник / Forestry Bulletin2542-1468Eco-Vector70653310.18698/2542-1468-2025-2-94-106Wood processing and chemical processing of woodДеревообработка и химическая переработка древесиныResearch ArticleLignosulfonates bioprocessingБиопереработка лигносульфонатовIvankinAndrey N.ИванкинАндрей Николаевич
Russian Federation

Dr. Sci. (Chem.), Member of The International Higher Education Academy Of Sciences (IHEAS), Professor of the Department of Chemistry

д-р хим. наук, академик МАН ВШ, профессор кафедры химии и химических технологий лесного комплекса

aivankin@bmstu.ruZarubinaAngella N.ЗарубинаАнжелла Николаевна
Russian Federation

Cand. Sci. (Tehn.), Associate Professor, Head of the Department of Chemistry and Chemical Technologies of the Forest Complex

канд. техн. наук, зав. кафедрой химии и химических технологий лесного комплекса

zarubina@bmstu.ruBMSTU (Mytishchi branch)МГТУ им. Н.Э. Баумана (Мытищинский филиал)15042025292941062104202621042026Copyright ©; 2025, Ivankin A.N., Zarubina A.N.Copyright ©; 2025, Иванкин А.Н., Зарубина А.Н.2025Ivankin A.N., Zarubina A.N.Иванкин А.Н., Зарубина А.Н.https://creativecommons.org/licenses/by/4.0https://journals.eco-vector.com/2542-1468/article/view/706533

The lignin liquors recycling method to obtain yeast biomass by using the producer of baker's yeast Saccharomyces cerevisiae in the course of biosynthesis on a nutrient medium including waste from the pulp and paper industry such as lignosulfonates is described. It is shown that lignosulfonate, as a product of processing wood biomass, can be utilized in the process of bioprocessing in the presence of yeast into nutritious biomass, which is a protein ingredient for subsequent balancing, for example, of animal feed. Analysis of the carbohydrate composition of free sugars in the lignosulfonate part of the initial nutrient medium showed that the ratio of the main carbohydrates: arabinose, galactose, glucose, mannose, xylose, ribose and lactose was 1 : 1,6 : 1,8 : 6,4 : 12,5 : 0,1 : 0,02, which is likely to be used as a nutrient component in biotechnology. The methodology of biosynthesis was determined, optimal conditions for the process using lignosulfonate culture fluid were found, including (g/l of tap water): ammonium hydrogen phosphate — 1; potassium dihydrogen phosphate — 2; potassium hydrogen phosphate — 0,1; potassium chloride — 1,5; magnesium sulfate — 0,5; ammonium sulfate — 3; lignosulfonate — 2,5. It was shown that the process of yeast cultivation at a temperature of 35…40°C and pH 5,0 allowed obtaining a suspension containing 12…15 g/l of raw yeast cells in 8…10 hours, which can then be separated and used. The study of the amino acid and fatty-acid composition of the biomass confirmed the high biological value of the product, which may be of interest for use in cattle breeding.

Описана методология утилизации лигниновых щелоков путем получения дрожжевой биомассы с использованием продуцента пекарских дрожжей Saccharomyces cerevisiae в ходе осуществления биосинтеза на питательной среде с отходами целлюлозно-бумажной промышленности — лигносульфонатами. Показано, что лигносульфонаты, как продукты переработки древесной биомассы, в процессе их биопереработки могут быть в присутствии дрожжей утилизированы в питательную биомассу, которая представляет собой белковый ингредиент для последующего балансирования, например, животных кормов. Приведен анализ углеводного состава свободных сахаров в лигносульфонатной части исходной питательной среды и показано, что в ней соотношение основных углеводов: арабинозы, галактозы, глюкозы, маннозы, ксилозы, рибозы и лактозы было равным 1 : 1,6 : 1,8 : 6,4 : 12,5 : 0,1 : 0,02, что перспективно для использования продукта в качестве питательного компонента в биотехнологии. Изложена методология ведения биосинтеза. Установлены оптимальные условия ведения процесса с использованием лигносульфонатной культуральной жидкости. Определен оптимальный состав питательной среды, включающей (г/л воды водопроводной): гидрофосфат аммония — 1; дигидрофосфат калия — 2; гидрофосфат калия — 0,1; хлорид калия — 1,5; сульфат магния — 0,5; сульфат аммония — 3; лигносульфонат — 2,5. Показано, что процесс культивирования дрожжей при температуре 35…40 °С и рН 5,0 позволял за 8…10 ч получать суспензию, содержащую 12…15 г/л сырых дрожжевых клеток, которые в дальнейшем можно сепарировать и использовать в практических целях. Изучены аминокислотный и жирнокислотный составы полученной биомассы, что подтвердило высокую биологическую ценность продукта, который может представлять интерес для использования в животноводстве.

pulp and paper industry wastelignosulfonatesbaker's yeast Saccharomyces cerevisiaebiorefiningотходы целлюлозно-бумажной промышленностилигносульфонатыпекарские дрожжи Saccharomyces cerevisiaeбиопереработкаWang Y., Lyu B., Fu H., Li J., Ji L., Gong H., Zhang R., Liu J., Yu H. The development process of plant-based meat alternatives: Raw material formulations and processing strategies. Food Research International, 2023, v. 167, no. 5, p. 112689. https://doi.org/10.1016/j.foodres.2023.112689Wang Y., Lyu B., Fu H., Li J., Ji L., Gong H., Zhang R., Liu J., Yu H. The development process of plant-based meat alternatives: Raw material formulations and processing strategies // Food Research International, 2023, v. 167, no. 5, p. 112689. https://doi.org/10.1016/j.foodres.2023.112689Zhang J., Meng Z., Cheng Q., Li Q., Zhang Y., Li L., Shi A., Wang Q. Plant-based meat substitutes by high-moisture extrusion: Visualizing the whole process in data systematically from raw material to the products. J. of Integrative Agriculture, 2022, v. 21, no. 8, pp. 2435–2444. https://doi.org/10.1016/S2095-3119(21)63892-3Zhang J., Meng Z., Cheng Q., Li Q., Zhang Y., Li L., Shi A., Wang Q. Plant-based meat substitutes by high- moisture extrusion: Visualizing the whole process in data systematically from raw material to the products // J. of Integrative Agriculture, 2022, v. 21, no. 8, pp. 2435–2444. https://doi.org/10.1016/S2095-3119(21)63892-3Leontovich V.P. Rastitel’nye otkhody i perspektiva ikh ispol’zovaniya [Plant waste and prospects for their use]. Kormoproizvodstvo [Feed Production], 2010, no. 1, pp. 44–46.Леонтович В.П. Растительные отходы и перспектива их использования // Кормопроизводство, 2010. № 1. С. 44–46.Wang Q., Zhang Y., Ma K. Study of the differences in collection scope of raw materials of biomass CHP plants caused by regional factors. J. of Environmental Management, 2024, v. 360, no. 6, p. 121106. https://doi.org/10.1016/j.jenvman.2024.121106Wang Q., Zhang Y., Ma K. Study of the differences in collection scope of raw materials of biomass CHP plants caused by regional factors // J. of Environmental Management, 2024, v. 360, no. 6, p. 121106. https://doi.org/10.1016/j.jenvman.2024.121106Zhang K., Zhang W., Xie W., Luo Y., Wei G. Investigation of mechanical properties, chemical composition and microstructure for composite cementitious materials containing waste powder recycled from asphalt mixing plants. J. of Building Engineering, 2024, v. 96, no. 11, p. 110362. https://doi.org/10.1016/j.jobe.2024.110362Zhang K., Zhang W., Xie W., Luo Y., Wei G. Investigation of mechanical properties, chemical composition and microstructure for composite cementitious materials containing waste powder recycled from asphalt mixing plants // J. of Building Engineering, 2024, v. 96, no. 11, p. 110362. https://doi.org/10.1016/j.jobe.2024.110362Kuznetsova T.G., Ivankin A.N., Kulikovsky A.V. Nanosensornyy analiz myasnogo syr’ya i rastitel’nykh ob’ektov [Nanosensory analysis of meat raw materials and plant objects]. Saarbrücken: LAP LAMBERT, 2012, 232 p.Кузнецова Т.Г., Иванкин А.Н., Куликовский А.В. Наносенсорный анализ мясного сырья и растительных объектов. Саарбрюккен: LAP LAMBERT, 2012. 232 с.Bouzid H.A., Ibourki M., Hamdouch F., Oubannin S., Asbbane A., Hallouch O., Bijla L., Koubachi J., Majourhat K., Gharby S. Moroccan aromatic and medicinal plants: A review of economy, ethnobotany, chemical composition, and biological activities of commonly used plants. Food and Humanity, 2024, v. 2, no. 5. https://doi.org/10.1016/j.foohum.2024.100259Bouzid H.A., Ibourki M., Hamdouch F., Oubannin S., Asbbane A., Hallouch O., Bijla L., Koubachi J., Majourhat K., Gharby S. Moroccan aromatic and medicinal plants: A review of economy, ethnobotany, chemical composition, and biological activities of commonly used plants // Food and Humanity, 2024, v. 2, no. 5. https://doi.org/10.1016/j.foohum.2024.100259Vaglica А., Porrello F., Ilardi V., Bruno M. The essential oil chemical composition of a rare ethnopharmacoligical plant. Natural Product Research. 2024, no. 7. https://doi.org/10.1080/14786419.2024.2377310Vaglica А., Porrello F., Ilardi V., Bruno M. The essential oil chemical composition of a rare ethnopharmacoligical plant // Natural Product Research. 2024, no. 7. https://doi.org/10.1080/14786419.2024.2377310Ivankin A.N., Chernukha I.M., Kuznetsova T.G. O kachestve rastitel’nykh i zhivotnykh zhirov [On the quality of vegetable and animal fats]. Maslozhirovaya promyshlennost’ [Oil and Fat Industry], 2007, no. 2, pp. 8–11.Иванкин А.Н., Чернуха И.М., Кузнецова Т.Г. О качестве растительных и животных жиров // Масложировая промышленность, 2007. № 2. С. 8–11.Puss K.K., Paaver P., Loog M., Salmar S. Ultrasound effect on a biorefinery lignin-cellulose mixture. Ultrasonics Sonochemistry, 2024, v. 111, no. 12, p. 107071. https://doi.org/10.1016/j.ultsonch.2024.107071Puss K.K., Paaver P., Loog M., Salmar S. Ultrasound effect on a biorefinery lignin-cellulose mixture // Ultrasonics Sonochemistry, 2024, v. 111, no. 12, p. 107071. https://doi.org/10.1016/j.ultsonch.2024.107071Lan H.N., Liu R.Y., Liu Z.H., Li X., Li B.Z., Yuan Y.J. Biological valorization of lignin to flavonoids. Biotechnology Advances, 2023, v. 64, no. 5, p. 108107. https://doi.org/10.1016/j.biotechadv.2023.108107Lan H.N., Liu R.Y., Liu Z.H., Li X., Li B.Z., Yuan Y.J. Biological valorization of lignin to flavonoids // Biotechnology Advances, 2023, v. 64, no. 5, p. 108107. https://doi.org/10.1016/j.biotechadv.2023.108107Pham C.D., Dang M.D.T., Ly T.B., Tran K.D., Vo N.T., Do N.H.N, Mai P.T., Le P.K. A review of the extraction methods and advanced applications of lignin-silica hybrids derived from natural sources. International J. of Biological Macromolecules, 2023, v. 230, no. 3, p. 123175. https://doi.org/10.1016/j.ijbiomac.2023.123175Pham C.D., Dang M.D.T., Ly T.B., Tran K.D., Vo N.T., Do N.H.N, Mai P.T., Le P.K. A review of the extraction methods and advanced applications of lignin-silica hybrids derived from natural sources // International J. of Biological Macromolecules, 2023, v. 230, no. 3, p. 123175. https://doi.org/10.1016/j.ijbiomac.2023.123175Teo H.L., Wahab R.A., Zainal-Abidin M.H., Mark-Lee W.F., Susanti E. Co-production of cellulose and lignin by Taguchi-optimized one-pot deep eutectic solvent-assisted ball milling pretreatment of raw oil palm leaves. International J. of Biological Macromolecules, 2024, v. 280, no. 11, p. 135787. https://doi.org/10.1016/j.ijbiomac.2024.135787Teo H.L., Wahab R.A., Zainal-Abidin M.H., Mark-Lee W.F., Susanti E. Co-production of cellulose and lignin by Taguchi-optimized one-pot deep eutectic solvent- assisted ball milling pretreatment of raw oilpalm leaves // International J. of Biological Macromolecules, 2024, v. 280, no. 11, p. 135787. https://doi.org/10.1016/j.ijbiomac.2024.135787Hou Q., Liu Z., Shi Z., Yang H., Wang D., Yang J. A deep eutectic solvent pretreatment with self-cleaning lignin droplets function to efficiently improve the enzymatic saccharification and ethanol production of bamboo residues. Industrial Crops and Products, 2024, v. 216, no. 9, p. 118730. https://doi.org/10.1016/j.indcrop.2024.118730Hou Q., Liu Z., Shi Z., Yang H., Wang D., Yang J. A deep eutectic solvent pretreatment with self-cleaning lignin droplets function to efficiently improve the enzymatic saccharification and ethanol production of bamboo residues // Industrial Crops and Products, 2024, v. 216, no. 9, p. 118730. https://doi.org/10.1016/j.indcrop.2024.118730Brienza F., Cannella D., Montesdeoca D., Cybulska I., Debecker D.P. A guide to lignin valorization in biorefineries: traditional, recent, and forthcoming approaches to convert raw lignocellulose into valuable materials and chemicals. RSC Sustainability, 2024, v. 2, no. 1, pp. 37–90. https://doi.org/10.1039/d3su00140gBrienza F., Cannella D., Montesdeoca D., Cybulska I., Debecker D.P. A guide to lignin valorization in biorefineries: traditional, recent, and forthcoming approaches to convert raw lignocellulose into valuable materials and chemicals // RSC Sustainability, 2024, v. 2, no. 1, pp. 37–90. https://doi.org/10.1039/d3su00140gFrias M., Reynoso S., Rambhia S., Noki G., Olson J., Stoeber B., Trajano H.L. Effect of incubation conditions of cellulase hydrolysis on mechanical pulp fibre morphology. Carbohydrate Polymers, 2024, v. 344, no. 10, p. 122529. https://doi.org/10.1016/j.carbpol.2024.122529Frias M., Reynoso S., Rambhia S., Noki G., Olson J., Stoeber B., Trajano H.L. Effect of incubation conditions of cellulase hydrolysis on mechanical pulp fibre morphology // Carbohydrate Polymers, 2024, v. 344, no. 10, p. 122529. https://doi.org/10.1016/j.carbpol.2024.122529Jia W., Zhou M., Yang C., Zhang H., Niu M., Shi H. Evaluating process of auto-hydrolysis prior to kraft pulping on production of chemical pulp for end used paper-grade products. J. of Bioresources and Bioproducts, 2022, v. 7, no. 8, pp. 180–189. https://doi.org/10.1016/j.jobab.2022.05.002Jia W., Zhou M., Yang C., Zhang H., Niu M., Shi H. Evaluating process of auto-hydrolysis prior to kraft pulping on production of chemical pulp for end used paper-grade products // J. of Bioresources and Bioproducts, 2022, v. 7, no. 8, pp. 180–189. https://doi.org/10.1016/j.jobab.2022.05.002Liu H., Xu S., Li H., HeY. Exploring the dual effect of sodium lignosulfonate-modified LDH treatment and heat processing on elevating corrosion and wear resistance of Ni-W composite coatings. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2024, v. 702, part 2, no. 12, p. 135076. https://doi.org/10.1016/j.colsurfa.2024.135076Liu H., Xu S., Li H., He Y. Exploring the dual effect of sodium lignosulfonate-modified LDH treatment and heat processing on elevating corrosion and wear resistance of Ni-W composite coatings // Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2024, v. 702, part 2, no. 12, p. 135076. https://doi.org/10.1016/j.colsurfa.2024.135076Demuner I.F., Gomes B.J.B., Gomes J.S., Coura M.R., Borges F.P., Carvalho A.M., Silva C.M. Improving kraft pulp mill sustainability by lignosulfonates production from processes residues. J. of Cleaner Production, 2021, v. 317, no. 10, p. 128286. https://doi.org/10.1016/j.jclepro.2021.128286Demuner I.F., Gomes B.J.B., Gomes J.S., Coura M.R., Borges F.P., Carvalho A.M., Silva C.M. Improving kraft pulp mill sustainability by lignosulfonates production from processes residues // J. of Cleaner Production, 2021, v. 317, no. 10, p. 128286. https://doi.org/10.1016/j.jclepro.2021.128286Song Y., Zhong S., Li Y., Dong K., Luo Y., Chu G., Zou H., Sun B. Study on the catalytic degradation of sodium lignosulfonate to aromatic aldehydes over nano-CuO: Process optimization and reaction kinetics. Chinese J. of Chemical Engineering, 2023, v. 53, no. 1, pp. 300–309. doi.org/10.1016/j.cjche.2021.12.028Song Y., Zhong S., Li Y., Dong K., Luo Y., Chu G., Zou H., Sun B. Study on the catalytic degradation of sodium lignosulfonate to aromatic aldehydes over nano-CuO: Process optimization and reaction kinetics // Chinese J. of Chemical Engineering, 2023, v. 53, no. 1, pp. 300–309. doi.org/10.1016/j.cjche.2021.12.028Wang J., Jiao H., Gao S., Wei J., Yu F., Xie C., Yuan B., Yu S. Potential high-energy-density biofuels from α-pinene and lignin-based phenols via alkylation and subsequent hydrodeoxygenation. Fuel, 2024, v. 374, no. 10, P. 132513. https://doi.org/10.1016/j.fuel.2024.132513Wang J., Jiao H., Gao S., Wei J., Yu F., Xie C., Yuan B., Yu S. Potential high-energy-density biofuels from α-pinene and lignin-based phenols via alkylation and subsequent hydrodeoxygenation // Fuel, 2024, v. 374, no. 10, P. 132513. https://doi.org/10.1016/j.fuel.2024.132513Liang G., Zhang S., Xian Y., Chen L. Depressing molybdenite using calcium lignosulfonate in Cu-Mo flotation separation: Interaction and desorption insights. Advanced Powder Technology, 2024, v. 35, no. 11, p. 104665. https://doi.org/10.1016/j.apt.2024.104665Liang G., Zhang S., Xian Y., Chen L. Depressing molybdenite using calcium lignosulfonate in Cu-Mo flotationseparation: Interactionand desorptioninsights// Advanced Powder Technology, 2024, v. 35, no. 11, p. 104665. https://doi.org/10.1016/j.apt.2024.104665Stanisz M., Smułek W., Popielski K., Klapiszewski L., Kaczorek E., Jesionowski T. Sustainable design of lignin-based spherical particles with the use of green surfactants and its application as sorbents in wastewater treatment. Chemical Engineering Research and Design, 2021, v. 172, no. 8, pp. 34–42. https://doi.org/10.1016/j.cherd.2021.05.028Stanisz M., Smułek W., Popielski K., Klapiszewski L., Kaczorek E., Jesionowski T. Sustainable design of lignin-based spherical particles with the use of green surfactants and its application as sorbents in wastewater treatment // Chemical Engineering Research and Design, 2021, v. 172, no. 8, pp. 34–42. https://doi.org/10.1016/j.cherd.2021.05.028Xu Y., Ding H., Luo C., Zheng Y., Xu Y., Li X., Zhang Z., Shen S., Zhang L. Effect of lignin, cellulose and hemicellulose on calcium looping behavior of CaO-based sorbents derived from extrusion-spherization method. Chemical Engineering J., 2018, v. 334, no. 2, pp. 2520–2529. https://doi.org/10.1016/j.cej.2017.11.160Xu Y., Ding H., Luo C., Zheng Y., Xu Y., Li X., Zhang Z., Shen S., Zhang L. Effect of lignin, cellulose and hemicellulose on calcium looping behavior of CaObased sorbents derived from extrusion-spherization method // Chemical Engineering J., 2018, v. 334, no. 2, pp. 2520–2529. https://doi.org/10.1016/j.cej.2017.11.160Patel R., Babaei-Ghazvini A., Dunlop M.J., Acharya B. Biomaterials-based concrete composites: A review on biochar, cellulose and lignin. Carbon Capture Science & Technology, 2024, v. 12, no. 9, p. 100232. https://doi.org/10.1016/j.ccst.2024.100232Patel R., Babaei-Ghazvini A., Dunlop M.J., Acharya B. Biomaterials-based concrete composites: A review on biochar, cellulose and lignin // Carbon Capture Science & Technology, 2024, v. 12, no. 9, p. 100232. https://doi.org/10.1016/j.ccst.2024.100232Yang S., Li Y., Yang Y., Liu R., Zhao Y. Behavior of calcium lignosulfonate under oxygen pressure acid leaching condition. Hydrometallurgy, 2024, v. 227, no. 8, p. 106317. https://doi.org/10.1016/j.hydromet.2024.106317Yang S., Li Y., Yang Y., Liu R., Zhao Y. Behavior of calcium lignosulfonate under oxygen pressure acid leaching condition // Hydrometallurgy, 2024, v. 227, no. 8, p. 106317. https://doi.org/10.1016/j.hydromet.2024.106317Feizi Z.H., Kazzaz A.E., Kong F., Fatehi P. Evolving a flocculation process for isolating lignosulfonate from solution. Separation and Purification Technology, 2019, v. 222, no. 9, pp. 254–263. https://doi.org/10.1016/j.seppur.2019.04.042Feizi Z.H., Kazzaz A.E., Kong F., Fatehi P. Evolving a flocculation process for isolating lignosulfonate from solution // Separation and Purification Technology, 2019, v. 222, no. 9, pp. 254–263. https://doi.org/10.1016/j.seppur.2019.04.042Cave G., Fatehi P. Separation of lignosulfonate from spent liquor of neutral sulphite semichemical pulping process via surfactant treatment. Separation and Purification Technology, 2015, v. 151, no. 9, pp. 39–46. https://doi.org/10.1016/j.seppur.2015.07.017Cave G., Fatehi P. Separation of lignosulfonate from spent liquor of neutral sulphite semichemical pulping process via surfactant treatment // Separation and Purification Technology, 2015, v. 151, no. 9, pp. 39–46. https://doi.org/10.1016/j.seppur.2015.07.017Yang J., Xing S., Yang W., Zhang A., Wang W. Application potential of modified waste-lignin as microbial immobilization carriers for improve soil fertility. Reactive and Functional Polymers, 2024, v. 196, no. 3, p. 105837. https://doi.org/10.1016/j.reactfunctpolym.2024.105837Yang J., Xing S., Yang W., Zhang A., Wang W. Application potential of modified waste-lignin as microbial immobilization carriers for improve soil fertility // Reactive and Functional Polymers, 2024, v. 196, no. 3, p. 105837. https://doi.org/10.1016/j.reactfunctpolym.2024.105837Kononov G.N. Dendrokhimiya. Khimiya, nanokhimiya i biogeokhimiya komponentov kletok, tkaney i organov drevesnykh rasteniy [Dendrochemistry. Chemistry, nanochemistry and biogeochemistry of components of cells, tissues and organs of woody plants]. In 2 vol. Moscow: MSFU, 2015, 1112 p.Кононов Г.Н. Дендрохимия. Химия, нанохимия и биогеохимия компонентов клеток, тканей и органов древесных растений, в 2 т. М: МГУЛ, 2015. 1112 с.Kononov G.N., Verevkin A.N., Serdyukova Ju.V., Mironov D.A. Drevesina kak khimicheskoe syr’e. Istoriya i sovremennost’. IV. Delignifikatsiya drevesiny kak put’ polucheniya tsellyulozy. Chast’ I [Wood as chemical raw material. History and modernity. IV. Wood delignification to produce cellulose. Part I]. Lesnoy vestnik / Forestry Bulletin, 2022, vol. 26, no. 1, pp. 97–113. DOI: 10.18698/2542-1468-2022-1-97-113Кононов Г.Н., Веревкин А.Н., Сердюкова Ю.В., Миронов Д.А. Древесина как химическое сырье. История и современность. IV. Делигнификация древесины как путь получения целлюлозы. Часть I // Лесной вестник / Forestry Bulletin, 2022. Т. 26. № 1. С. 97–113. DOI: 10.18698/2542-1468-2022-1-97-113Kiviniemi Е., Mikkola A., Mattila H., Wahlsten M., Lundell T. Oxidative stress and culture atmosphere effects on bioactive compounds and laccase activity in the white rot fungus Phlebia radiata on birch wood substrate. Current Research in Microbial Sciences, 2024, v. 7, p. 100280. https://doi.org/10.1016/j.crmicr.2024.100280Kiviniemi Е., Mikkola A., Mattila H., Wahlsten M., Lundell T. Oxidative stress and culture atmosphere effects on bioactive compounds and laccase activity in the white rot fungus Phlebia radiata on birch wood substrate // Current Research in Microbial Sciences, 2024, v. 7, p. 100280. https://doi.org/10.1016/j.crmicr.2024.100280Grellet M.A., Dantur K.I., Perera M.F., Ahmed P.M., Castagnaro A., Arroyo-Lopez F.N., Gallego J.B., Welin B., Ruiz M.R. Genotypic and phenotypic characterization of industrial autochthonous Saccharomyces cerevisiae for the selection of well-adapted bioethanol-producing strains. Fungal Biology, 2022, v. 126, no. 10, pp. 658–673. https://doi.org/10.1016/j.funbio.2022.08.004Grellet M.A., Dantur K.I., Perera M.F., Ahmed P.M., Castagnaro A., Arroyo-Lopez F.N., Gallego J.B., Welin B., Ruiz M.R. Genotypic and phenotypic characterization of industrial autochthonous Saccharomyces cerevisiae for the selection of well-adapted bioethanol-producing strains // Fungal Biology, 2022, v. 126, no. 10, pp. 658–673. https://doi.org/10.1016/j.funbio.2022.08.004Ivankin A.N., Oliferenko G.L., Kulikovsky A.V. Analiticheskaya himiya [Analytical chemistry]. Moscow: Knorus, 2021, 300 p.Иванкин А.Н., Олиференко Г.Л., Куликовский А.В. Аналитическая химия. М.: КНОРУС, 2021. 300 с.GOST R 51880–2002. Opredelenie massovykh doley svobodnykh i obshchikh uglevodov. Metod vysokoeffektivnoy anionnoy khromatografii [Determination of mass fractions of free and total carbohydrates. High performance anion chromatography method] Moscow: Standards, 2002, 12 p.ГОСТ Р 51880–2002. Определение массовых долей свободных и общих углеводов. Метод высокоэффективной анионной хроматографии. М.: Издательство стандартов, 2002. 12 с.LisitsynA.B., IvankinA.N., NeklyudovA.D. Metody prakticheskoy biotekhnologii [Methods of practical biotechnology]. Moscow: VNIIMP, 2002, 408 p.Лисицын А.Б., Иванкин А.Н., Неклюдов А.Д. Методы практической биотехнологии. М.: Изд-во ВНИИМП, 2002. 408 с.Huang C., Jeuck B., Du J., Yong Q., Chang H., Jameel H., Phillips R. Novel process for the coproduction of xylooligosaccharides, fermentable sugars, and lignosulfonates from hardwood. Bioresource Technology, 2016, v. 219, no. 11, pp. 600–607. https://doi.org/10.1016/j.biortech.2016.08.051Huang C., Jeuck B., Du J., Yong Q., Chang H., Jameel H., Phillips R. Novel process for the coproduction of xylo-oligosaccharides, fermentable sugars, and lignosulfonates from hardwood // Bioresource Technology, 2016, v. 219, no. 11, pp. 600–607. https://doi.org/10.1016/j.biortech.2016.08.051Wang Z., Li X., Liu H., Zhou T., Li J., Siddiqui M.A. Enhanced short-chain fatty acids production from anaerobic fermentation of secondary sludge by lignosulfonate addition: Towards circular economy. J. of Cleaner Production, 2024, v. 434, no. 1, p. 140252. https://doi.org/10.1016/j.jclepro.2023.140252Wang Z., Li X., Liu H., Zhou T., Li J., Siddiqui M.A. Enhanced short-chain fatty acids production from anaerobic fermentation of secondary sludge by lignosulfonate addition: Towards circular economy // J. of Cleaner Production, 2024, v. 434, no. 1, p. 140252. https://doi.org/10.1016/j.jclepro.2023.140252Meledina T.V., Davydenko S.G. Drozhzhi Saccharomyces cerevisiae. Morfologiya, khimicheskiy sostav, metabolizm. [Yeast Saccharomyces cerevisiae. Morphology, chemical composition, metabolism]. St. Petersburg: ITMO University, 2015, 88 p.Меледина Т.В., Давыденко С.Г. Дрожжи Saccharomyces cerevisiae. Морфология, химический состав, метаболизм. СПб.: Изд-во Университета ИТМО, 2015. 88 с.Kosolapov V.M., Chuikov V.A., Khudyakova H.K., Kosolapova V.G. Mineral’nye elementy v kormakh i metody ikh analiza [Mineral elements in feed and methods of their analysis]. Moscow: Ugreshskaya Printing House, 2019, 272 p.Косолапов В.М., Чуйков В.А., Худякова Х.К., Косолапова В.Г. Минеральные элементы в кормах и методы их анализа. М.: Угрешская типография, 2019. 272 с.Morales-Palomo S., Tomás-Pejó E., González-Fernández C. Phosphate chelation over calcium impacts yeast growth and lipid production from short-chain fatty acids-rich media. Environmental Technology & Innovation, 2024, v. 36, no. 11, p. 103767. https://doi.org/10.1016/j.eti.2024.103767Morales-Palomo S., Tomás-Pejó E., González-Fernández C. Phosphate chelation over calcium impacts yeast growth and lipid production from short-chain fatty acids-rich media // Environmental Technology & Innovation, 2024, v. 36, no. 11, p. 103767. https://doi.org/10.1016/j.eti.2024.103767Huang Н., Xu J., Sheng Z., Xie R., Zhang H., Chen N., Li S. Effects of dietary phospholipids on growth performance, fatty acid composition, and expression of lipid metabolism related genes of juvenile hybrid. Aquaculture Reports, 2022, v. 22, no. 2, p. 100993. https://doi.org/10.1016/j.aqrep.2021.100993Huang Н., Xu J., Sheng Z., Xie R., Zhang H., Chen N., Li S. Effects of dietary phospholipids on growth performance, fatty acid composition, and expression of lipid metabolism related genes of juvenile hybrid // Aquaculture Reports, 2022, v. 22, no. 2, p. 100993. https://doi.org/10.1016/j.aqrep.2021.100993Veerasamy V., Neethirajan V., Singarayar M.S., Balasundaram D., Dharmar P., Thilagar S. Microalgal biomass and lipid synergy for omega fatty acid enrichment: A sustainable source for food supplements & nutraceuticals. Algal Research, v. 80, no. 6, p. 103514. https://doi.org/10.1016/j.algal.2024.103514Veerasamy V., Neethirajan V., Singarayar M.S., Balasundaram D., Dharmar P., Thilagar S. Microalgal biomass and lipid synergy for omega fatty acid enrichment: A sustainable source for food supplements & nutraceuticals // Algal Research, v. 80, no. 6, p. 103514. https://doi.org/10.1016/j.algal.2024.103514Di Caprio F. Cultivation processes to select microorganisms with high accumulation ability. Biotechnology Advances, 2021, v. 49, no. 7, p. 107740. doi.org/10.1016/j.biotechadv.2021.107740Di Caprio F. Cultivation processes to select microorganisms with high accumulation ability // Biotechnology Advances, 2021, v. 49, no. 7, p. 107740. doi.org/10.1016/j.biotechadv.2021.107740Deive F.J., Sanroman M.A. Bioreactor development for the cultivation of extremophilic microorganisms. Current Developments in Biotechnology and Bioengineering. Bioprocesses, Bioreactors and Controls. Eds. Larroche C. et al. Elsevier, 2017, 816 p. doi.org/10.1016/B978-0-444-63663-8.00014-8Deive F.J., Sanroman M.A. Bioreactor development for the cultivation of extremophilic microorganisms // Current Developments in Biotechnology and Bioengineering. Bioprocesses, Bioreactors and Controls / Eds. Larroche C. et al. Elsevier, 2017, 816 p. doi.org/10.1016/B978-0-444-63663-8.00014-8Xu J., Zhou J., Du B, Li X., Huang Y., Cao Q., Xu S., Wang W. Research progress on the preparation and application of lignin-based Pickering emulsions: A review. Industrial Crops and Products, 2024, v. 222, no. 12, p. 119723. doi.org/10.1016/j.indcrop.2024.119723Xu J., Zhou J., Du B, Li X., Huang Y., Cao Q., Xu S., Wang W. Research progress on the preparation and application of lignin-based Pickering emulsions: A review // Industrial Crops and Products, 2024, v. 222, no. 12, p. 119723. doi.org/10.1016/j.indcrop.2024.119723Ivankin A.N., Oliferenko G.L. Corrosion inhibition with green polymer systems and natural compounds. Polymer Science, Series D, 2024, v. 17, no. 4, pp. 987–994. DOI: 10.1134/S1995421224701697Ivankin A.N., Oliferenko G.L. Corrosion inhibition with green polymer systems and natural compounds // Polymer Science, Series D, 2024, v. 17, no. 4, pp. 987–994. DOI: 10.1134/S1995421224701697Oliferenko G.L., Ivankin A.N., Ustyugov A.V. Kislotnaya degradatsiya drevesnykh otkhodov kak sposob polucheniya tselevykh produktov [Wood waste acid degradation to obtain target products]. Lesnoy vestnik / Forestry Bulletin, 2024, vol. 28, no. 4, pp. 130–137. DOI: 10.18698/2542-1468-2024-4-130-137Иванкин А.Н., Олиференко Г.Л., Устюгов А.В. Кислотная деградация древесных отходов как способ получения целевых продуктов // Лесной вестник / Forestry Bulletin, 2024. Т. 28. № 4. С. 130–137. DOI: 10.18698/2542-1468-2024-4-130-137Wang J., Seidi F., Shi X., Li C., Huang Y., Xiao H. Unveiling the potential of dual-extrinsic/intrinsic self-healing ligninbased coatings for anticorrosion applications. International J. of Biological Macromolecules, 2025, v. 285, no. 1, p. 138073. doi.org/10.1016/j.ijbiomac.2024.138073Wang J., Seidi F., Shi X., Li C., Huang Y., Xiao H. Unveiling the potential of dual-extrinsic/intrinsic self-healing lignin-based coatings for anticorrosion applications // International Journal of Biological Macromolecules, 2025, v. 285, no. 1, p. 138073. doi.org/10.1016/j.ijbiomac.2024.138073Yin Y., Qin S., Deng S., Li Z., Tang A., Li Q., Liao D., Liu Y. Thermoresponsive lignin-based polyelectrolyte complexes for the preparation of spherical nanoparticles: Application in pesticide encapsulation. International J. of Biological Macromolecules, 2025, v. 288, no. 2, p. 138623. doi.org/10.1016/j.ijbiomac.2024.138623Yin Y., Qin S., Deng S., Li Z., Tang A., Li Q., Liao D., Liu Y. Thermoresponsive lignin-based polyelectrolyte complexes for the preparation of spherical nanoparticles: Application in pesticide encapsulation // International Journal of Biological Macromolecules, 2025, v. 288, no. 2, p. 138623. doi.org/10.1016/j.ijbiomac.2024.138623