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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="research-article" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">Lesnoy Vestnik / Forestry Bulletin</journal-id><journal-title-group><journal-title xml:lang="en">Lesnoy Vestnik / Forestry Bulletin</journal-title><trans-title-group xml:lang="ru"><trans-title>Лесной вестник / Forestry Bulletin</trans-title></trans-title-group></journal-title-group><issn publication-format="print">2542-1468</issn><publisher><publisher-name xml:lang="en">Eco-Vector</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">706764</article-id><article-id pub-id-type="doi">10.18698/2542-1468-2024-5-94-103</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>Forest crops, breeding and genetics</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>Лесные культуры, селекция и генетика</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title xml:lang="en">Regeneration and rooting features of <italic>Ribes uva-crispa</italic> L. cultivars <italic>in vitro</italic></article-title><trans-title-group xml:lang="ru"><trans-title>Особенности регенерации и укоренения сортов крыжовника обыкновенного <italic>Ribes uva-crispa</italic> L. в культуре in vitro</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Krakhmaleva</surname><given-names>Irina L.</given-names></name><name xml:lang="ru"><surname>Крахмалева</surname><given-names>Ирина Леонидовна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>Junior researcher of Plant Biotechnology Laboratory</p></bio><bio xml:lang="ru"><p>мл. науч. сотр. лаборатории биотехнологии растений</p></bio><email>seglory@bk.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Koroleva</surname><given-names>Ol’ga V.</given-names></name><name xml:lang="ru"><surname>Королева</surname><given-names>Ольга Васильевна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>Researcher of Plant Biotechnology Laboratory</p></bio><bio xml:lang="ru"><p>науч. сотр. лаборатории биотехнологии растений</p></bio><email>elaem@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Molkanova</surname><given-names>Ol’ga I.</given-names></name><name xml:lang="ru"><surname>Молканова</surname><given-names>Ольга Ивановна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>Cand. Sc. (Agr.), Leading researcher, Head of Plant Biotechnology Laboratory</p></bio><bio xml:lang="ru"><p>канд. с.-х. наук, вед. науч. сотр., заведующий лабораторией биотехнологии растений</p></bio><email>molkanova@mail.ru</email><xref ref-type="aff" rid="aff1"/></contrib></contrib-group><aff-alternatives id="aff1"><aff><institution xml:lang="en">Tsitsin Main Botanical Garden of the Russian Academy of Sciences</institution></aff><aff><institution xml:lang="ru">ФГБУН «Главный ботанический сад им. Н.В. Цицина Российской академии наук» (ГБС РАН)</institution></aff></aff-alternatives><pub-date date-type="pub" iso-8601-date="2024-10-26" publication-format="electronic"><day>26</day><month>10</month><year>2024</year></pub-date><volume>28</volume><issue>5</issue><issue-title xml:lang="en"/><issue-title xml:lang="ru"/><fpage>94</fpage><lpage>103</lpage><history><date date-type="received" iso-8601-date="2026-04-25"><day>25</day><month>04</month><year>2026</year></date><date date-type="accepted" iso-8601-date="2026-04-25"><day>25</day><month>04</month><year>2026</year></date></history><permissions><copyright-statement xml:lang="en">Copyright ©; 2024, Krakhmaleva I.L., Koroleva O.V., Molkanova O.I.</copyright-statement><copyright-statement xml:lang="ru">Copyright ©; 2024, Крахмалева И.Л., Королева О.В., Молканова О.И.</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="en">Krakhmaleva I.L., Koroleva O.V., Molkanova O.I.</copyright-holder><copyright-holder xml:lang="ru">Крахмалева И.Л., Королева О.В., Молканова О.И.</copyright-holder><ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/"/><license><ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">https://creativecommons.org/licenses/by/4.0</ali:license_ref></license></permissions><self-uri xlink:href="https://journals.eco-vector.com/2542-1468/article/view/706764">https://journals.eco-vector.com/2542-1468/article/view/706764</self-uri><abstract xml:lang="en"><p>The article focuses on optimizing medium composition for promising gooseberry (<italic>Ribes uva-crispa</italic> L.) cultivars at the multiplication and rooting stages. The research was carried out in the Laboratory of Plant Biotechnology of Tsitsin Main Botanical Garden of Russian Academy of Sciences in 2022. Cultivars ‘Berill’, ‘Grushen’ka’, ‘Kseniya’ and ‘Chernoslivovy’ were used in the experiments. The effect of iron chelate forms (Fe(III)-EDDHA and Fe(III)-EDTA) on morphogenetic parameters and development of gooseberry microshoots was determined. At the micropropagation stage addition of 100 mg/L Fe(III)-EDDHA to Quorin and Lepoivre medium increased the height of microshoots of the studied cultivars. The number of microshoots available for further rooting (10 mm height and above) ranged from 20 to 37 % on the media containing 100,0 mg/L Fe(III)-EDDHA compared to the media with 36,7 mg/L Fe(III)-EDTA (10...16 %). The highest morphogenetic potential was found in ‘Chernoslivovy’, which had 1,4…2,1 times higher multiplication rate than other cultivars. Rooting ability and morphometric parameters of root system were found influenced by genetic characteristics of gooseberry cultivars, type and concentration of auxins in half-strength Murashige and Skoog medium. Using of the media with 0,5...1,5 mg/L of Indolyl-3-butyric acid encouraged the formation of higher number of roots, whereas the media with 0,5...1,5 mg/L of Indolyl-3-acetic acid increased their length. Usage of medium with 0,5 mg/L Indole-3-butyric acid was found to be the most effective for rooting of most studied cultivars.</p></abstract><trans-abstract xml:lang="ru"><p>Статья посвящена оптимизации составов питательных сред на этапах собственно микроразмножения и укоренения перспективных сортов крыжовника обыкновенного (<italic>Ribes</italic><italic> uva</italic><italic>-crispa</italic> L.). Исследования проводили в 2022 г. в лаборатории биотехнологии растений Главного ботанического сада им. Н.В. Цицина Российской академии наук. В качестве объектов использовали сорта: ‘Берилл’, ‘Грушенька’, ‘Ксения’ и ‘Черносливовый’. Определено влияние хелатных форм железа (Fe(III)-EDDHA и Fe(III)-EDTA) на морфогенетические показатели и развитие микропобегов крыжовника. Установлено, что на этапе собственно микроразмножения добавление 100 мг/л хелата Fe(III)-EDDHA в питательную среду Quorin and Lepoivre увеличивало высоту микропобегов исследуемых сортов. Число микропобегов, пригодных для дальнейшего укоренения (высотой 10 мм и выше), на средах с 100 мг/л Fe(III)-EDDHA составило от 20 до 37 %, по сравнению со средой с 36,7 мг/л Fe(III)-EDTA — от 10 до 16 %. Выявлено, что наибольшим морфогенетическим потенциалом характеризовался сорт ‘Черносливовый’, у которого коэффициент размножения был больше в 1,4–2,1 раза, чем у других сортов. Установлено, что на укореняемость и морфометрические параметры корневой системы влияли сортовые особенности крыжовника, тип и концентрация ауксинов в питательной среде 1/2 Murashige and Skoog. Показано, что использование питательных сред с 0,5…1,5 мг/л индолил-3-масляной кислотой способствовало образованию большего числа корней, а с 0,5…1,5 мг/л индолил-3-уксусной кислотой увеличивало их длину. Установлено, что использование питательной среды с добавлением 0,5 мг/л индолил-3-масляной кислоты было наиболее эффективным для укоренения большинства исследуемых сортов.</p></trans-abstract><kwd-group xml:lang="en"><kwd>Ribes uva-crispa L.</kwd><kwd>clonal micropropagation</kwd><kwd>multiplication rate</kwd><kwd>rhizogenesis</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>крыжовник обыкновенный Ribes uva-crispa L.</kwd><kwd>клональное микроразмножение</kwd><kwd>коэффициент размножения</kwd><kwd>ризогенез</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution xml:lang="ru">Министерство науки и высшего образования Российской Федерации</institution></institution-wrap><institution-wrap><institution xml:lang="en">Ministry of Science and Higher Education of the Russian Federation</institution></institution-wrap></funding-source><award-id>075-00745-22-01</award-id></award-group><funding-statement xml:lang="en">The work was carried out under the state assignment of MBG RAS (No. 122042700002-6).</funding-statement><funding-statement xml:lang="ru">Работа выполнена в рамках государственного задания ГБС РАН (№ 122042700002-6).</funding-statement></funding-group></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>Wang H., Cao G., Prior R.L. 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