Lesnoy Vestnik / Forestry BulletinLesnoy Vestnik / Forestry BulletinЛесной вестник / Forestry Bulletin2542-1468Eco-Vector70696510.18698/2542-1468-2024-3-105-114Wood processing and chemical processing of woodДеревообработка и химическая переработка древесиныResearch ArticleOptimisation of plant protein hydrolysis in presence of yeast proteasesОптимизация процесса гидролиза растительных белков в присутствии дрожжевых протеазIvankinAndrey N.ИванкинАндрей Николаевич
Russian Federation

Dr. Sci. (Chem.), Member of the International Higher Education Academy of Sciences (IHEAS), Professor of the Department of Chemistry

д-р хим. наук, академик МАН ВШ, профессор кафедры химии и химических технологий лесного комплекса

aivankin@mgul.ac.ruBMSTU (Mytishchi branch)ФГБОУ ВО «Московский государственный технический университет имени Н.Э. Баумана (национальный исследовательский университет)» (Мытищинский филиал)290620242831051142804202628042026Copyright ©; 2024, Ivankin A.N.Copyright ©; 2024, Иванкин А.Н.2024Ivankin A.N.Иванкин А.Н.https://creativecommons.org/licenses/by/4.0https://journals.eco-vector.com/2542-1468/article/view/706965

A methodology is described for optimizing the process of enzymatic hydrolysis of plant proteins by yeast proteases Saccharomyces carlsbergensis using model soy protein as a substrate. Enzymatic treatment was carried out under the influence of autolyzed brewer’s yeast waste containing up to 40 % active yeast cells. It is shown that as a result of process optimization using the simplex method of experiment planning, optimal conditions for conducting the process of enzymatic hydrolysis of plant proteins in the presence of brewer’s yeast biomass were found, which were: protein concentration in the suspension 30 %, amount of added yeast suspension 40 %, sodium citrate content 5,6 %, ethanol 4,2 %, added water 20,2 %. The time of hydrolytic treatment at the optimum temperature of 58 °C was 4,3 hours. The product yield, assessed by the nitrogen content of free amino groups, increased from 10...12 % without optimization to 34 % as a result of the optimization of process parameters. It has been shown that the maximum rate of the process of hydrolytic decomposition of plant proteins under optimal conditions is more than 0,48∙10–3 mgNH2/ml∙min, and the activation energy Ea is 80,7 kJ/mol. The possibility of additionally increasing the yield of the target hydrolyzate by re-introducing the enzyme preparation pancreatin into the system was established, as a result of which it was possible to increase the yield of the product to 52...55 % and obtain a product balanced in amino acid composition. It was found that the amino acid composition of the resulting enzymatic hydrolyzate included all essential amino acids, g/100 g protein: Ile 4,7; Leu 7,0; Lys 6,9; Met 1,6; Cys 3,7; Phe 5,8; Tyr 4,1; Tre 4,2; Trp 1,5; Val 6,8; as well as non-essential amino acids: Ala 5,9; Arg 5,5; Asp 8,8; His 3,3; Gly 3,8; Glu 10,2; Pro 5,1; Ser 9,0. It has been shown that the enzymatic hydrolyzate has potentially high biological value, which can facilitate its effective use as part of highly nutritious systems.

Дано описание методологии оптимизации процесса ферментативного гидролиза растительных белков дрожжевыми протеазами Saccharomyces carlsbergensis с использованием в качестве субстрата модельного соевого белка. Ферментативную обработку осуществляли под действием автолизованных отходов пивных дрожжей, содержащих до 40 % активных дрожжевых клеток. Показано, что в результате оптимизации процесса с использованием симплекс метода планирования эксперимента были найдены оптимальные условия ведения процесса ферментативного гидролиза растительных белков в присутствии биомассы пивных дрожжей, которые составили: концентрация белка в суспензии 30 %, количество добавляемой дрожжевой суспензии 40 %, содержание цитрата натрия 5,6 %, этанола 4,2 %, добавленной воды 20,2 %. Время гидролитической обработки в оптимуме при температуре 58 °С составляло 4,3 часа. Выход продукта, оцениваемый по содержанию азота свободных аминогрупп, увеличивался с 10…12 % без оптимизации до 34 % в результате проведенной оптимизации параметров процесса. Показано, что максимальная скорость процесса гидролитического распада растительных белков в оптимальных условиях составляет более 0,48∙10–3 мгNH2/мл∙мин, а энергия активации Ea 80,7 кДж/моль. Установлена возможность дополнительного увеличения выхода целевого гидролизата за счет повторного внесения в систему ферментного препарата панкреатина, в результате чего удавалось увеличивать выход продукта до 52…55 % и получать сбалансированный по аминокислотному составу продукт. Получены данные, о том, что аминокислотный состав ферментативного гидролизата включал все незаменимые аминокислоты, г/100 г белка: Иле 4,7; Лей 7,0; Лиз 6,9; Мет 1,6; Цис 3,7; Фен 5,8; Тир 4,1; Тре 4,2; Трп 1,5; Вал 6,8; а также заменимые аминокислоты: Ала 5,9; Арг 5,5; Асп 8,8; Гис 3,3; Гли 3,8; Глу 10,2; Про 5,1; Сер 9,0. Полученный ферментативный гидролизат обладает потенциально высокой биологической ценностью, что может способствовать его эффективному использованию в составе высокопитательных систем.

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