Doklady Biological SciencesDoklady Biological SciencesДоклады Российской академии наук. Науки о жизни2686-73893034-5057The Russian Academy of Sciences68404510.31857/S2686738925020115ArticlesСтатьиResearch ArticleIdentification of a highly conserved region critical for the functionality of the CP190 protein in Drosophila melanogasterИдентификация высоко консервативного района критичного для функциональности белка CP190 у Drosophila melanogasterMelnikovaL. S.МельниковаЛ. С.
Russian Federation
lsm73@mail.ruBalagurovK. I.БалагуровК. И.
Russian Federation
lsm73@mail.ruGeorgievP. G.ГеоргиевП. Г.
Russian Federation

Academician of the RAS

академик РАН

lsm73@mail.ruGolovninA. K.ГоловнинА. К.
Russian Federation
lsm73@mail.ruInstitute of Gene Biology, Russian Academy of SciencesФедеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук (ИБГ РАН)1504202552112292341206202512062025Copyright ©; 2025, Russian Academy of SciencesCopyright ©; 2025, Российская академия наук2025Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/2686-7389/article/view/684045

The CP190 protein binds to both housekeeping gene promoters and insulator/boundary elements and plays a critical role in their activity. The aim of this work was to study the effect of deletions in highly conserved regions of the CP190 protein on its functionality. It was shown that deletion of the sequence from 664 to 700 aa leads to a lethal phenotype. Thus, a new region was identified in CP190 that plays an important role in the interaction of the CP190 protein with as yet unidentified partner proteins, stabilization of protein complexes formed by CP190 and their recruitment to chromatin.

Белок СP190 связывается как с промоторами генов домашнего хозяйства, так и с инсуляторными/граничными элементами и играет критическую роль в их активности. Целью работы являлось изучение влияния делеций в высоко консервативных районах белка CP190 на его функциональность. Показано, что делеция последовательности от 664 до 700 а.о. приводит к летальному фенотипу. Таким образом, в составе СР190 выявлен новый район, играющий важную роль во взаимодействии белка СР190 с еще не идентифицированными белками-партнерами, стабилизации формируемых СP190 белковых комплексов и рекрутировании их на хроматин.

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