Doklady Biological SciencesDoklady Biological SciencesДоклады Российской академии наук. Науки о жизни2686-73893034-5057The Russian Academy of Sciences68407210.31857/S2686738925020176ArticlesСтатьиResearch ArticleRegenerative potential of platelet-rich plasma in diabetes mellitusРегенеративный потенциал обогащенной тромбоцитами плазмы при сахарном диабетеVlasovaT. I.ВласоваТ. И.
Russian Federation
v.t.i@bk.ruBrodovskayaE. P.БродовскаяЕ. П.
Russian Federation
v.t.i@bk.ruMadonovК. S.МадоновК. С.
Russian Federation
v.t.i@bk.ruAgeevV. P.АгеевВ. П.
Russian Federation
v.t.i@bk.ruAbelovaA. P.АбеловаА. П.
Russian Federation
v.t.i@bk.ruPinyaevS. I.ПиняевС. И.
Russian Federation
v.t.i@bk.ruVlasovА. P.ВласовА. П.
Russian Federation
v.t.i@bk.ruFederal State Budgetary Educational Institution of Higher Education “National Research Ogarev Mordovia State University”Федеральное государственное бюджетное образовательное учреждение высшего образования “Национальный исследовательский Мордовский государственный университет им. Н. П. Огарёва”1504202552112682791206202512062025Copyright ©; 2025, Russian Academy of SciencesCopyright ©; 2025, Российская академия наук2025Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/2686-7389/article/view/684072

The study explores in vitro the regenerative potential of platelet-rich plasma (PRP) from patients with type 1 diabetes mellitus: in a series of experiments they add PRP to the culture medium of dermal fibroblasts and evaluate in dynamics the metabolic (including the production of reactive oxygen species), migration and proliferation activity of the cells, and they also determine the growth factors and exosomes levels in PRP and the medium. They reveal a decrease in the proliferative, prooxidant and toxic effects of PRP from patients with diabetes mellitus. They are characterized with a decrease in metabolic activity and cell viability in culture with an increase in the percentage of necrosis, while the migration properties of the cells were not impaired. The presence of diabetes mellitus in a patient is also accompanied by a decrease in the stimulating effect of his PRP on dermal fibroblasts in the secretion of growth factors. These effects were most significant in elderly people with diabetes.

В исследовании изучен in vitro регенеративный потенциал обогащенной тромбоцитами плазмы (PRP), полученной от пациентов с сахарным диабетом двух возрастных групп: в серии экспериментов PRP вносили в культуральную среду дермальных фибробластов человека (ДФЧ) и в динамике оценивали метаболическую (в т. ч. продукцию активных форм кислорода), миграционную и пролиферативную активность клеток; определяли уровни факторов роста и экзосом в PRP и культуральной среде. Выявлено снижение пролиферативного влияния, прооксидантное действие и токсические эффекты PRP пациентов с сахарным диабетом, что характеризовалось снижением метаболической активности и жизнеспособности клеток в культуре с увеличением процента некроза, при этом миграционные свойства ДФЧ не нарушались. Наличие сахарного диабета у пациента также сопровождается снижением стимулирующего действия его PRP на ДФЧ в секреции факторов роста. Наибольшей выраженности данные эффекты достигали у лиц с СД пожилого возраста.

platelet-rich plasmaPRP-therapyregenerative medicinepersonalized medicinediabetes mellitusобогащенная тромбоцитами плазмаPRP-терапиярегенеративная медицинаперсонализированная медицинасахарный диабетРоссийский научный фондRussian Science Foundation24-25-00278

Presented by Academician of the RAS I.V. Reshetov

Представлено академиком РАН И.В. Решетовым

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