Doklady Biological SciencesDoklady Biological SciencesДоклады Российской академии наук. Науки о жизни2686-73893034-5057The Russian Academy of Sciences69350310.31857/S2686738925040098ArticlesСтатьиResearch ArticlePreparation of 6-azido-2-chloropurine-2′-deoxyriboside by enzymatic transglycosylation reaction catalyzed by Lactobacillus leichmannii type 2 nucleoside deoxyribosyltransferaseПолучение 6-азидо-2-хлорпурин-2′-дезоксирибозида с помощью реакции ферментативного трансгликозилирования, катализируемой нуклеозиддезоксирибозилтрансферазой второго типа Lactobacillus leichmanniiAlexeevC. S.АлексеевК. С.micelle@mail.ruKonkinaM. A.КонкинаМ. А.cyril.alex@eimb.ruKurochkinN. N.КурочкинН. Н.micelle@mail.ruDrenichevM. S.ДреничевМ. С.micelle@mail.ruEngelhardt Institute of Molecular Biology, Russian Academy of SciencesИнститут молекулярной биологии им. В. А. Энгельгардта Российской академии наукLomonosov Institute of Fine Chemical Technologies, MIREA Russia Technological UniversityИнститут тонких химических технологий имени М. В. Ломоносова, “МИРЭА – Российский технологический университет”150820255231VOL 523, NO (2025)ТОМ 523, № (2025)43143816102025Copyright ©; 2025, Russian Academy of SciencesCopyright ©; 2025, Российская академия наук2025Russian Academy of SciencesРоссийская академия наукhttps://journals.eco-vector.com/2686-7389/article/view/693503

6-azido-2-chloropurine-2′-deoxyriboside, a valuable precursor for the preparation of modified 2-chloropurine nucleosides substituted at the 6-position of the heterocyclic base, was obtained by enzymatic transglycosylation. 6-azido-2-chloropurine-2′-deoxyriboside can also be used as a photocross-linking agent to study the nucleic acids – proteins interactions. A type 2 nucleoside deoxyribosyltransferase from Lactobacillus leichmannii was used as a biocatalyst. The optimal conditions for the formation of 6-azido-2-chloropurine-2′-deoxyriboside using 7-methyl-2′-deoxyguanosine as a carbohydrate residue donor were determined.

6-azido-2-chloropurine-2′-deoxyriboside, a valuable precursor for the preparation of modified 2-chloropurine nucleosides substituted at the 6-position of the heterocyclic base, was obtained by enzymatic transglycosylation. 6-azido-2-chloropurine-2′-deoxyriboside can also be used as a photocross-linking agent to study the nucleic acids – proteins interactions. A type 2 nucleoside deoxyribosyltransferase from Lactobacillus leichmannii was used as a biocatalyst. The optimal conditions for the formation of 6-azido-2-chloropurine-2′-deoxyriboside using 7-methyl-2′-deoxyguanosine as a carbohydrate residue donor were determined.

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