Recent Advances in Anti-Infective Drug DiscoveryRecent Advances in Anti-Infective Drug DiscoveryRecent Advances in Anti-Infective Drug Discovery2772-43442772-4344Bentham Science64457410.2174/0127724344251369231212061409MedicineResearch ArticleExpression of mRNA-TLR-5 Gene in Patients with Endometriosis using Real-time PCR in Tehran, IranRaghibiMaryaminfo@benthamscience.netRaghibiMalihehinfo@benthamscience.netMorovvatiAbbasinfo@benthamscience.netShakibPegahinfo@benthamscience.netAflatoonianRezainfo@benthamscience.netDirbaiyanAshkaninfo@benthamscience.netDepartment of Microbiology, Qom Branch, Islamic Azad UniversityRazi Herbal Medicines Research Center, Lorestan University of Medical ScienceDepartment of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine0104202419430030607012025Copyright ©; 2024, Bentham Science Publishers2024Bentham Science Publishershttps://journals.eco-vector.com/2772-4344/article/view/644574

Background:Endometriosis is one of the common diseases of women, especially in reproductive age, and it is one of the most important causes of infertility in women. The aim of this study was to investigate the level of mRNA-TLR-5 expression in women with endometriosis.

Methods:The present study was performed in Nikan Hospital, Tehran, Iran, in 2021. The samples of endometrial mucosa for the eutopic group and an ovarian endometriotic cyst for the ectopic group were obtained from the patients who underwent laparoscopic surgery at the Fetal Infertility Center and were diagnosed with endometriosis. Normal endometrial samples were also obtained from patients who had no history of infertility and underwent laparoscopic TL surgery for reasons other than endometriosis such as ovarian cysts (control group). After RNA extraction and cDNA synthesis, TLR-5 gene expression was evaluated by the Real-Time PCR method.

Results:Based on the results of the comparison of TLR-5 gene expression in all three ectopic, eutopic endometrium, and control groups by Real-Time PCR, it was found that the TLR-5 gene expression is significantly higher in ectopic samples than in the other two groups, but there is a significant difference between two utopic and control groups.

Conclusion:The increase in TLR-5 expression in the ectopic group can probably be a reason for reducing the apoptosis of cells entered into the peritoneal cavity and creating an environment for the survival and proliferation of these cells.

Endometrial mucosagene expressionTLR-5 genereal-time polymerase chain reaction (Real-Time PCR)glands and stromaabdomen.Fritz MA, Speroff L. Clinical gynecologic endocrinology and infertility: lippincott Williams & wilkins 2011.Gardner R, Raemer DB. Simulation in obstetrics and gynecology. Obstet Gynecol Clin North Am 2008; 35(1): 97-127. ix. doi: 10.1016/j.ogc.2007.12.008 PMID: 18319131Vannuccini S, Reis FM, Coutinho LM, Lazzeri L, Centini G, Petraglia F. Surgical treatment of endometriosis: Prognostic factors for better quality of life. Gynecol Endocrinol 2019; 35(11): 1010-4. doi: 10.1080/09513590.2019.1616688 PMID: 31155975Bozdag G. Recurrence of endometriosis: Risk factors, mechanisms and biomarkers. Womens Health 2015; 11(5): 693-9. doi: 10.2217/whe.15.56 PMID: 26439119Berkkanoglu M, Arici A. Immunology and Endometriosis. Am J Reprod Immunol 2003; 50(1): 48-59. doi: 10.1034/j.1600-0897.2003.00042.x PMID: 14506928Vallvé-Juanico J, Houshdaran S, Giudice LC. The endometrial immune environment of women with endometriosis. Hum Reprod Update 2019; 25(5): 565-92. doi: 10.1093/humupd/dmz018 PMID: 31424502Riccio LGC, Santulli P, Marcellin L, Abrão MS, Batteux F, Chapron C. Immunology of endometriosis. Best Pract Res Clin Obstet Gynaecol 2018; 50: 39-49. doi: 10.1016/j.bpobgyn.2018.01.010 PMID: 29506962Izumi G, Koga K, Takamura M, et al. Involvement of immune cells in the pathogenesis of endometriosis. J Obstet Gynaecol Res 2018; 44(2): 191-8. doi: 10.1111/jog.13559 PMID: 29316073de Azevedo BC, Mansur F, Podgaec S. A systematic review of toll-like receptors in endometriosis. Arch Gynecol Obstet 2021; 304(2): 309-16. doi: 10.1007/s00404-021-06075-x PMID: 33928453Arabian S, Boostan A, Darzi S. The role of toll-like receptors (TLRs) and their therapeutic applications in endometrial cancer. Clin Transl Oncol 2022; 25(4): 859-65. doi: 10.1007/s12094-022-02999-1 PMID: 36374404Kobayashi H, Higashiura Y, Shigetomi H, Kajihara H. Pathogenesis of endometriosis: The role of initial infection and subsequent sterile inflammation (Review). Mol Med Rep 2014; 9(1): 9-15. doi: 10.3892/mmr.2013.1755 PMID: 24173432Majewska M, Szczepanik M. The role of Toll-like receptors (TLR) in innate and adaptive immune responses and their function in immune response regulation. Postepy Hig Med Dosw 2006; 60: 52-63. PMID: 16474276Gewirtz AT, Navas TA, Lyons S, Godowski PJ, Madara JL. Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression. J Immunol 2001; 167(4): 1882-5. doi: 10.4049/jimmunol.167.4.1882 PMID: 11489966Rock FL, Hardiman G, Timans JC, Kastelein RA, Bazan JF. A family of human receptors structurally related to Drosophila Toll. Proc Natl Acad Sci 1998; 95(2): 588-93. doi: 10.1073/pnas.95.2.588 PMID: 9435236Allhorn S, Böِing C, Koch AA, Kimmig R, Gashaw I. TLR3 and TLR4 expression in healthy and diseased human endometrium. Reprod Biol Endocrinol 2008; 6(1): 40. doi: 10.1186/1477-7827-6-40Singh G, Kumar S, Singh P. A quick method to isolate RNA from wheat and other carbohydrate-rich seeds. Plant Mol Biol Report 2003; 21(1): 93. doi: 10.1007/BF02773401Basu M, Paichha M, Swain B, et al. Modulation of TLR2, TLR4, TLR-5 , NOD1 and NOD2 receptor gene expressions and their downstream signaling molecules following thermal stress in the Indian major carp catla. 3 Biotech 2015; 5(6): 1021-30.Abdelsadik A, Trad A. Toll-like receptors on the fork roads between innate and adaptive immunity. Hum Immunol 2011; 72(12): 1188-93. doi: 10.1016/j.humimm.2011.08.015 PMID: 21920397Symons LK, Miller JE, Kay VR, et al. The immunopathophysiology of endometriosis. Trends Mol Med 2018; 24(9): 748-62. doi: 10.1016/j.molmed.2018.07.004 PMID: 30054239Moghaddam MZ, Ansariniya H, Seifati SM, Zare F, Fesahat F. Immunopathogenesis of endometriosis: An overview of the role of innate and adaptive immune cells and their mediators. Am J Reprod Immunol 2022; 87(5): e13537. doi: 10.1111/aji.13537 PMID: 35263479Li X, Jiang S, Tapping RI. Toll-like receptor signaling in cell proliferation and survival. Cytokine 2010; 49(1): 1-9. doi: 10.1016/j.cyto.2009.08.010 PMID: 19775907Fazeli A, Bruce C, Anumba DO. Characterization of Toll-like receptors in the female reproductive tract in humans. Hum Reprod 2005; 20(5): 1372-8. doi: 10.1093/humrep/deh775 PMID: 15695310Aboussahoud W, Aflatoonian R, Bruce C, et al. Expression and function of Toll-like receptors in human endometrial epithelial cell lines. J Reprod Immunol 2010; 84(1): 41-51. doi: 10.1016/j.jri.2009.09.008 PMID: 19959241Dogan S, Koester F, Agic A, Eds. Expression of Toll-like Receptor 3 (TLR3) and induction of apoptosis in patients with endometriosis and healthy controls Human Reproduction Oxford Ox2 6dp. England: Oxford Univ Press Great Clarendon St 2007.Beilmann-Lehtonen I, Hagströِm J, Mustonen H, Koskensalo S, Haglund C, Böِckelman C. High tissue TLR-5 expression predicts better outcomes in colorectal cancer patients. Oncology 2021; 99(9): 589-600. doi: 10.1159/000516543 PMID: 34139707Stanislawowski M, Wierzbicki PM, Golab A, et al. Decreased Toll-like receptor-5 (TLR-5) expression in the mucosa of ulcerative colitis patients. J Physiol Pharmacol 2009; 60: 71-5. PMID: 20083854