Recent Advances in Anti-Infective Drug DiscoveryRecent Advances in Anti-Infective Drug DiscoveryRecent Advances in Anti-Infective Drug Discovery2772-43442772-4344Bentham Science64457610.2174/0127724344274260231220052526MedicineResearch ArticleInvestigation of EpsA, OmpA, and Bap Genes among MDR and XDR Acinetobacter baumannii Isolates in Khorramabad, IranPoladiImaninfo@benthamscience.netShakibPegahinfo@benthamscience.netHalimiShahnazinfo@benthamscience.netDelfaniSomayehinfo@benthamscience.netZadehFarzadinfo@benthamscience.netRezaeiFaranakinfo@benthamscience.netSchool of Medicine, Lorestan University of Medical ScienceRazi Herbal Medicines Research Center, Lorestan University of Medical SciencesDepartment of Microbiology, School of Medicine, Tehran University of Medical ScienceDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesNutritional Health Research Center, Department of Biostatistics and Epidemiology, School of Health and Nutrition, Lorestan University of Medical Sciences0104202419430731407012025Copyright ©; 2024, Bentham Science Publishers2024Bentham Science Publishershttps://journals.eco-vector.com/2772-4344/article/view/644576

Background:Acinetobacter baumannii is an opportunistic hospital pathogen with high antibiotic resistance, and the ability to produce biofilm. This study aimed to investigate epsA, ompA, and bap genes involved in biofilm formation in MDR and XDR clinical isolates of Acinetobacter baumannii in Khorramabad, Iran.

Methods:In this study, 79 A. baumannii isolates were collected from various samples of the patients admitted to tertiary hospitals in Khorramabad city, Iran, between January and August 2019. After performing the semi-quantitative evaluation of biofilm production by microtiter plate assay, screening of isolates carrying epsA, ompA, and bap genes was done by PCR method. Finally, statistical analyses were conducted using SPSS 22.

Results:Among 79 A.baumannii isolates, 52% XDR, 40% MDR, and 16% non-XDRMDR isolates were found to be biofilm producers. All XDR and 94% MDR isolates had ompA and epsA genes, and bap genes were detected among > 80% of these isolates. Moreover, the presence of biofilm-related genes and biofilm production among non-XDRMDR isolates were less than among resistant isolates (p≤ 0.01).

Conclusion:Based on the results, biofilm production and simultaneous presence of epsA, ompA, and bap genes among MDR, and XDR A. baumannii isolates have been found to be significantly more than non-XDR-MDR isolates.

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