Medical academic journalMedical academic journal1608-41012687-1378Eco-Vector1093610.17816/MAJ18377-84Research ArticleEvaluation of the radioprotective properties of recombinant flagellin when used alone or in combination with interleukin-1 betaMurzinaE V-SofronovG A-SimbirtsevA S-AksenovaN V-VeselovaO M-IschenkoA M-S.M. Kirov Military Medical AcademyInstitute of Experimental MedicineResearch Institute of Highly Pure Biopreparations15092018183778423012019Copyright © 2018, Murzina E.V., Sofronov G.A., Simbirtsev A.S., Aksenova N.V., Veselova O.M., Ischenko A.M.2018Research objective. To evaluate the radioprotective effectiveness of recombinant flagellin when used alone or in combination with interleukin-1 beta for prophylactic or therapeutic effect on animals. Materials and methods. The effect of hybrid flagellin FliC Salmonella typhimurium and human interleukin-1β (Institute of Highly Pure Biopreparations, Saint Petersburg, Russia) on the 30-day survival of male mice exposed to lethal doses of X-ray radiation was studied. Survival of irradiated animals was analyzed by Kaplan-Meier method. Results. The preventive use of flagellin had a protective effect on the 30-day survival of mice irradiated with lethal doses of X-rays. Compared with the irradiated control, the administration of flagellin (1 mg/kg or 2 mg/kg) prior to X-ray exposure (7.5 Gy, 8.0 Gy or 8.5 Gy) increased the animal survival rate to 67-87%. Complex preventive administration of flagellin (1 mg/kg) and interleukin-1 beta (50 μg/kg) provided a 100% survival rate of the irradiated mice. Separate use of drugs was also effective; 92.8% of mice survived when flagellin was administered prior to irradiation and interleukin after. Conclusion. 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