Medical academic journalMedical academic journal1608-41012687-1378Eco-Vector1519110.17816/MAJ19165-72Research ArticleThe effect methylglyoxal on acute lung injury induced by influenza A(H1N1)PDM09 in miceAleksandrovAndrei G.<p>a graduate student in the Laboratory of Drug Safety</p>forphchemistry@gmail.comhttps://orcid.org/0000-0001-9212-3865Savateeva-LyubimovaTatiana N.<p>Professor, MD, PhD in Medicine, Leading Researcher in the Laboratory of Drug Safety</p>drugs_safety@mail.ruhttps://orcid.org/0000-0003-4516-3308MuzhikyanArman A.<p>PhD in Veterinary Sciences, Leading Researcher in the Laboratory of Drug Safety</p>vetdiagnostics.spb@gmail.comhttps://orcid.org/0000-0002-7093-0014Smorodintsev Research Institute of Influenza0207201919165720207201902072019Copyright © 2019, Aleksandrov A.G., Savateeva-Lyubimova T.N., Muzhikyan A.A.2019<p><strong><em>The aim of the article.</em></strong> To study of the effect of 2-oxopropanal (methylglyoxal) on virus-induced acute lung injury.</p>
<p><strong><em>Materials and methods.</em></strong> The study was performed on adult female outbred mice. Methylglyoxal administered subcutaneously at a dose of 50 mg/kg/day to mice for 14 days prior to infection. The pandemic influenza virus A(H1N1)pdm09 was used for modeling viral infection at a dose of 0.75 LD<sub>50</sub>. Hematology, pathomorphological and histological studies were performed on 4, 7 and 14 days post infection. Level of lung injury was performed by semi-quantitative method.</p>
<p><strong><em>Results.</em></strong> Methylglyoxal induced 2-fold increase of mortality and lung lesion area (<em>р</em> 0.05). The structural changes in lung tissue had inflammatory character. These changes had progressive character. The ratio of neutrophiles/lymphocytes was increased by 2.5 times on average in infected animals (<em>р</em> 0.05 compared to intact animals).</p>
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